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Plant regeneration from callus and protoplast cultures of Lotus pedunculatus Cav.

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Abstract

Plant regeneration from leaf- and cotyledon-derived calli and from protoplast-derived tissue has been obtained in Lotus pedunculatus. Callus induction was achieved with 2,4-D and plant regeneration required the following two media sequences: bud formation was stimulated by IAA and BA and shoot growth by kinetin. Root formation occurred in the presence of IAA. Cotyledon protoplasts showed a low plating efficiency and plant regeneration was achieved via an intervening callus phase.

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Abbreviations

BA:

6-benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

2iP:

N6-Δ-2-isopentenyl-adenine

NAA:

α-naphthaleneacetic acid

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Authors and Affiliations

  1. Centro di Studio per il Miglioramento Genetico delle Piante Foraggere del CNR, Borgo XX Giugno 74, 06100, Perugia, Italy

    F. Pupilli, S. Arcioni, F. Damiani & M. Pezzotti

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  1. F. Pupilli
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  2. S. Arcioni
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  3. F. Damiani
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  4. M. Pezzotti
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Pupilli, F., Arcioni, S., Damiani, F. et al. Plant regeneration from callus and protoplast cultures of Lotus pedunculatus Cav.. Plant Cell Tiss Organ Cult 23, 193–199 (1990). https://doi.org/10.1007/BF00034431

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  • DOI: https://doi.org/10.1007/BF00034431

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