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In vitro differentiation of plantlets from embryonic explants of lodgepole pine (Pinus contorta Dougl. ex Loud.)

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Abstract

A protocol is described for plantlet formation in juvenile tissues of Pinus contorta. Shoots were induced on embryonic, cotyledonary and hypocotyl explants cultured on a defined medium supplemented with cytokinin. The concentration of salts, vitamins and cytokinin (benzylamino purine) in the medium, as well as different temperature regimes, strongly influenced the frequency of bud formation. Differentiation of shoot primordia and their subsequent development was also markedly affected by cytokinin exposure times. Bud development and elongation were enhanced by elimination of the phytohormone, reducing the strength of mineral salts, vitamins and sucrose in the medium, as well as by the inclusion of charcoal. Rooting was induced by treating the shoots with a sterilized rooting powder containing indole-butyric acid and culturing them in agar-solidified medium containing reduced mineral salts, vitamins, sucrose and charcoal. The number of chromosomes and their structure were found to be normal in the regenerated plantlets.

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Authors and Affiliations

  1. Plant Physiology Research Group, Department of Biology, University of Calgary, T2N 1N4, Calgary, Alberta, Canada

    Kamlesh R. Patel & Trevor A. Thorpe

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  1. Kamlesh R. Patel
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  2. Trevor A. Thorpe
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Patel, K.R., Thorpe, T.A. In vitro differentiation of plantlets from embryonic explants of lodgepole pine (Pinus contorta Dougl. ex Loud.). Plant Cell Tiss Organ Cult 3, 131–142 (1984). https://doi.org/10.1007/BF00033734

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  • DOI: https://doi.org/10.1007/BF00033734

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