Abstract
Protoplast isolation from apple leaf tissue of in-vitro cultured shoots is possible if very young leaves from buds are used. Digestible leaves are found in apple shoots of the subculture Åkerö 14 days after transferring the shoot and of M26 some days later.
The yield of protoplasts is considerably improved if the apple shoots are cultured in a medium containing L-methionine (0.5 mM).
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Huang S-C, Millikan DF (1983) Isolation of protoplasts of apple leaf tissue. In: Randall DD, Blevins DG, Larson RL, Rapp BJ (eds) Current topics in plant biochemistry and physiology, p 249
Kouider M, Hauptmann R, Wdholm JM, Skirvin M, Korban SS (1984) Callus formation from Malus x omestica cv ‘Jonathan’ protoplasts. Plant Cell Reports 3: 142–145
James DJ, Passey AJ, Malhotra SB (1984) Isolation and fusion of protoplasts. East Malling Research Station Report for 1983: 63–65
Welander M, Huntrieser I (1981) The rooting ability of shoots raised ‘in vitro’ from the apple rootstock A2 in juvenile and in adult growth phase. Physiol Plant 53: 301–306
Welander M (1985) In vitro shoot and root formation in the apple cultivar Åkerö. Ann Bot 55: 249–261
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Menczel L, Nagy F, Kiss Zs R, Maliga P (1981) Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: Correlation of resistance to N. tobacum plastids. Theor Appl Genet 59: 191–195
Hegnauer R (1973) Chemotaxonomie der Pflanzen Vol 6, p 117
Cassel AC, Barlass M (1976) Environmentally induced changes in the cell walls of tomato leaves in relation to cell and protoplast release. Physiol Plant 37: 239–246
Killick KA (1971) Modification of the composition and structure of the yeast cell wall by culture in the presence of sulfur amino acids. J Bacteriaol 106: 931–937
Wallin A, Glimelius K, Eriksson T (1977) Pretreatment of cell suspensions as a method to increase the protoplast yield of Haplopappus gracilis. Physiol Plant 40: 307–311
James DJ, Passey AJ, Malhotra SB, Deeming DC (1983) Isolation and fusion of protoplasts from apple and cherry tissues. East Malling Research Station Report for 1982: 150–152
Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126: 105–110
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Wallin, A., Welander, M. Improved yield of apple leaf protoplasts from in vitro cultured shoots by using very young leaves and adding L-methionine to the shoot medium. Plant Cell Tiss Organ Cult 5, 69–72 (1985). https://doi.org/10.1007/BF00033571
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DOI: https://doi.org/10.1007/BF00033571