Abstract
A photoacoustic cell assembly is described that is permeable to CO2 and other gases but not water vapor. As a replacement for the usually employed solid cover, this cell uses a cover containing a small fritted glass disk that holds a small piece of 6.4 μm Teflon film against the sample.
With the above arrangement it was possible to increase the rate of O2 evolution measured photoacoustically about 3 times in Zea mays leaves and about 1.7 times in Phaseolus vulgaris leaves upon adding CO2 to the gas stream. The extent of energy storage was also enhanced with supplemental CO2 in Zea and Ulva but less so in Phaseolus. The maximum improvements of photosynthetic activities were obtained when the gas stream contained 2.5–5% CO2. These high concentrations were presumably necessary as the result of a high resistance to diffusion through the gas-permeable cover.
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Abbreviations
- ES:
-
energy storage
- PA:
-
photoacoustic
- RuBP:
-
ribulose-1,5-bisphosphate carboxylase
References
Bento AC, Aguiar MMF, Vargas H, daSilva MD, Bandeira IN and Miranda LCM (1989) Open photoacoustic cell X-ray detection. Appl Phys B 48: 269–272
Buschmann C, Prehn H and Lichtenthaler H (1984) Photoacoustic spectroscopy (PAS) and its application in photosynthesis research. Photosyn Res 5: 29–46
daSilva MD, Bandeira IN and Miranda LCM (1987) Open-cell photoacoustic radiation detector. J Phys E: Instrum 30: 1476–1478
Malkin S, Herbert SK and Fork DC (1990) Light distribution, transfer and utilization in the marine alga Porphyra perforata from photoacoustic energy-storage measurements. Biochim Biophys Acta 1016: 177–189
Malkin S, Lasser-Ross N, Bults G and Cahen J (1981) Photoacoustic spectroscopy in photosynthesis. In: Akoyunoglou G (ed) Photosynthesis III. Structure and Molecular Organization of the Photosynthetic Apparatus, pp 1031–1042. Philadelphia: Balaban Inter. Science Services
Marquezini MV, Cella N, Silva EC, Serra DB, Lima CAS and Vargas H (1990) Photoacoustic assessment of the in vivo genotypical response of corn to toxic aluminium. Analyst 115: 341–343
Osmond CB, Björkman O and Anderson DJ (1980) Physiological Processes in Plant Ecology. Towards a Synthesis with Atriplex. Berlin: Springer Verlag
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Fork, D.C., Herbert, S.K. A gas-permeable photoacoustic cell. Photosynth Res 27, 151–156 (1991). https://doi.org/10.1007/BF00033254
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DOI: https://doi.org/10.1007/BF00033254