Skip to main content
SpringerLink
Log in

A study on the energy-dependent quenching of chlorophyll fluorescence by means of photoacoustic measurements

  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

The mechanism of energy-dependent quenching (qE) of chlorophyll fluorescence was studied employing photoacoustic measurements of oxygen evolution and heat release. It is shown that concomitant to the formation of qE the yield of open reaction centers φp decreases indicating that qE quenching originates from a process being competitive to fluorescence as well as to photochemistry. The analysis of heat release (rate of thermal deactivation) shows: 1. The competitive process is not given by a still unknown energy storing process. 2. If the competitive process would be a futile cycle the life-times of the involved intermediates had to be faster than 50 μs.

The results of the photoacoustic measurements are in line with the idea that qE quenching originates from an increased probability of thermal deactivation of excited chlorophylls.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

F:

actual fluorescence

F′m :

fluorescence yield with all PS II reaction centers closed in a light adapted state

F′0 :

fluorescence yield with all PS II reaction centers open in a light adapted state

PS:

Photosystem

φp :

intrinsic photochemical yield

qE :

energy-dependent quenching

qI :

photoinhibition quenching

qN :

non-photochemical quenching

qP :

photochemical quenching

qT :

state transition quenching

References

  • Bradbury M and Baker NR (1984) A quantitative determination of photochemical and non-photochemical quenching during the slow phase of chlorophyll fluorescence induction curves of bean leaves. Biochim Biophys Acta 765: 275–284

    Google Scholar 

  • Briantais J-M, Vernotte C, Picaud M and Krause GH (1979) A quantitative study on the slow decline of chlorophyll a fluorescence in isolated chloroplasts. Biochim Biophys Acta 548: 123–138

    Google Scholar 

  • Briantais J-M, Vernotte C, Krause GH and Weis E (1986) Chlorophyll a fluorescence of higher plants: chloroplasts and leaves. In: Govindjee, Amesz J and Fork DC (eds) Light Emission by plants and bacteria, pp 539–586. Orlando: Academic Press

    Google Scholar 

  • Buschmann C (1990) Photoacoustic spectroscopy and its application in Plant Science. Bot Acta 103: 9–14

    Google Scholar 

  • Bults G, Horwitz B, Malkin S and Cahen D (1981) Frequency-dependent photoacoustic signals from leaves and their relations to photosynthesis. FEBS Lett 129: 44–46

    Article  Google Scholar 

  • Bults G, Horwitz B, Malkin S and Cahen D (1982) Photoacoustic measurements of photosynthetic activities in whole leaves, photochemistry and gas exchange. Biochim Biophys Acta 679: 452–465

    Google Scholar 

  • Cahen D (1981) Photoacoustic cell for reflection and transition measurements. Rev Sci Instr 52: 1306–1310

    Article  Google Scholar 

  • Canaani O, Malkin S, Mauzerall D (1988) Pulsed-photoacoustic detection of flash induced oxygen evolution from intact leaves and its oscillations. Proc Natl Acad Sci (USA) 85: 4725–4729

    Google Scholar 

  • Dau H and Hansen UP (1988) The involvement of spillover changes in State1-State 2 transitions in intact leaves at low light intensities. Biochim Biophys Acta 934: 156–159

    Google Scholar 

  • Dau H and Hansen UP (1989) Studies on the adaptation of intact leaves to changing light intensities by a kinetic analysis of chlorophyll fluorescence and oxygen evolution as measured by the photoacoustic signal. Photosynth Res 20: 59–83

    Article  Google Scholar 

  • Dau H and Canaani O (1989) The involvement of LHC phosphorylation in the adaptation of higher plants to changing light intensities and some results on the regulation of LHC 2 phosphorylation in vivo. In: Baltscheffsky M (ed) Current Research in Photosynthesis, Vol IV, pp 325–328

  • Hansen UP, Kolbowski J and Dau H (1987) Relationship between photosynthesis and plasmalemma transport. J Exp Bot 38: 1965–1981

    Google Scholar 

  • Horton P and Hague A (1988) Studies on the function of chlorophyll fluorescence in isolated barley protoplasts. IV. Resolution of non-photochemical quenching. Biochim Biophys Acta 932: 107–115

    Google Scholar 

  • Krause GH, Vernotte C and Briantias J-M (1982) Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Biochim Biophys Acta 679: 116–124

    Google Scholar 

  • Krause GH, Briantais JM and Vernotte C (1983) Characterization of chlorophyll fluorescence quenching in chloroplasts by fluorescence spectroscopy at 77K. Biochim Biophys Acta 723: 169–175

    Google Scholar 

  • Krause GH and Weis E (1984) Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynth Res 5: 139–157

    Google Scholar 

  • Laasch H (1987) Non-photochemical quenching of chlorophyll a fluorescence in isolated chloroplasts under conditions of stressed photosynthesis. Planta 171: 220–226

    Google Scholar 

  • Malkin S and Cahen D (1979) Photoacoustic spectroscopy and radient energy conversion: Theory of the effect with special emphasis on photosynthesis. Photobiochem Photobiol 29: 803–813

    Google Scholar 

  • Oxborough K and Horton P (1988) A study of the regulation of energy-dependent quenching in pea chloroplasts. Biochim Biophys Acta 934: 135–143

    Google Scholar 

  • Peterson RB, Sivak MN and Walker DA (1988) Relationship between steady-state fluorescence yield and photosynthetic efficiency in leaf tissue. Plant Physiol 88: 158–163

    Google Scholar 

  • Poulet P, Cahen D and Malkin S (1983) Photoacoustic detection of photosynthetic oxygen evolution from leaves. Quantitative analysis by phase and amplitude measurements. Biochim Biophys Acta 724: 433–446

    Google Scholar 

  • Quick WP and Horton P (1984) Studies on the induction of chlorophyll fluorescence in barley. II. Resolution of fluorescence quenching by redox state and the transthylakoid pH gradient. Proc Roy Soc B 220: 371–382

    Google Scholar 

  • Schreiber U, Schliwa U and Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51–62

    Google Scholar 

  • Vanselow KH, Dau H and Hansen UP (1988) Indication of transthylakoid proton-fluxes in Aegopodium podagraria L. by light-induced changes of plasmalemma potential, chlorophyll fluorescence and light-scattering. Planta 176: 351–361

    Google Scholar 

  • Vanselow KH, Kolbowski J and Hansen UP (1989) Further evidence for the relationship between light-induced changes of plasmalemma transport and transthylakoid proton uptake. J Exp Bot 40: 239–245

    Google Scholar 

  • Weis E and Berry JA (1987) Quantum efficiency of Photosystem 2 in relation to energy dependent quenching of chlorophyll-fluorescence. Biochim Biophys Acta 894: 189–208

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut fuer Angewandte Physik, Universitaet Kiel, Leibnizstr. 11, 2300, Kiel, FRG

    Holger Dau & Ulf-Peter Hansen

Authors
  1. Holger Dau
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ulf-Peter Hansen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dau, H., Hansen, UP. A study on the energy-dependent quenching of chlorophyll fluorescence by means of photoacoustic measurements. Photosynth Res 25, 269–278 (1990). https://doi.org/10.1007/BF00033168

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00033168

Key words

Search

Navigation