Abstract
A detailed quantitative study of the kinetics of photochemical and non-photochemical quenching was achieved by a linear analysis of the yields of chlorophyll fluorescence and of oxygen evolution (as measured by the photoacoustic effect) by their responses to sinusoidal changes of actinic light. The results of this analysis were given in terms of the parameters of the kinetic phases obtained as a response to a step function change in the light intensity from a previous steady-state. Thus, it was possible to split the responses to a change in light intensity into six components which could be assigned to 6 time-constants (60 ms, 1.8 s, 2.5 s, 8 s, 150 s and 400 s). The comparison of the kinetics of responses induced by blue-light (approx. 400–500 nm) and by far-red (720 nm) light led to the assignment of the 1.8-s time-constant to the loading and discharge of the plastoquinone pool and of the 400-s time-constant to the state-transition controller which could be shown to be involved also in the adaptation to changes in light intensity and not only to changes in light quality (wavelength). The time-constant of 8 s, also occurring in 532-nm light-scattering was assigned to the “high-energy state” quenching (qE) of fluorescence. qE was paralleled by a decrease of the photoacoustic signal, demonstrating an “high-energy state” quenching of oxygen evolution as well. The 60-ms time-constant is suggested to be related to the redox state of the primary quinone acceptor of PS II, whereas the other two time-constants could not be identified. The calculation of the relative contributions of the photo-chemical and of the non-photochemical quenching in the individual components revealed that both quenching-mechanisms occur in all components except in the assumed fastest one.
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Abbreviations
- pa-signal:
-
photoacoustic signal
- PS:
-
photosystem
References
Allen, FA, Bennett, J, Steinback, KE and Arntzen, CJ (1981) Chloroplast protein phosphorylation couples plastochinone redox state to distribution of excitation energy between photosystems. Nature 291: 25–29
Bennoun, P and Li, YS (1973) New results on the mode of action of 3,-(3,4-dichlorophenyl)-1,1-dimethylurea in spinach chloroplasts. Biochim Biophys Acta 292: 162–168
Bode, HW (1964) Network analysis and feedback amplifier design. D. van Nostrand Comp. Inc. New York
Bonaventura, C and Myers, J (1969) Fluorescence and oxygen evolution from Chlorella Pyrenoidosa. Biochim Biophys Acta 767: 568–573
Bradbury, M and Baker, NR (1984) A quantitative determination of photochemical and non-photochemical quenching during the slow phase of chlorophyll fluorescence induction curves of bean leaves. Biochim Biophys Acta 765: 275–284
Briantais, J-M, Vernotte, C, Krause, GH and Weis, E (1986) Chlorophyll — a fluorescence of higher plants: chloroplasts and leaves. In: Govindjee, Amesz J and Fork, DC (eds) Light Emission by Plants and Bacteria, pp. 539–586, Orlando: Academic Press
Bults, G, Horwitz, B, Malkin, S and Cahen, D (1981) Frequency-dependent photoacoustic signals from leaves and their relations to photosynthesis. FEBS Lett 129: 44–46
Bults, G, Horwitz, B, Malkin, S and Cahen, D (1982) Photoacoustic measurements of photosynthetic activities in whole leaves, photochemistry and gas exchange. Biochim Biophys Acta 679: 452–465
Buschmann, C and Grumbach, K (1985) Physiologie der Photosynthese. Springer Verlag Berlin, Heidelberg. pp 100–101
Butler, WL (1978) Energy distribution in the photochemical apparatus of photosynthesis. Ann Rev Plant Physiol 29: 345–378
Cahen, D (1981) Photoacoustic cell for reflection and transition measurements. Rev Sci Instr 52: 1306–1310
Canaani, O, Barber, J and Malkin, S (1984a) Evidence that phosphorylation and dephosphorylation regulate the distribution of excitation energy between the two photosystems of photosynthesis in vivo: Photoacoustic and fluorimetric study of an intact leaf. Proc Natl Acad Sci (USA) 81: 1614–1618
Canaani, O and Malkin, S (1984b) Distribution of light excitation in an intact leaf between the two photosystems of photosynthesis — Changes in absorption cross-sections following statel-state 2 transitions. Biochim Biophys Acta 766 (1984): 513–524
Christoffers, D (1986) On the theory of in vivo chlorophyll-fluorescence. Computer simulation of primary photosynthetic reactions. Photobiochim Photobiophys 11: 101–113
Dau H and Hansen UP (1988) Kinetic responses of the oxygen evolution induced by blue and by far-red light. In: Hess P and Pelzl P (eds) Photoacoustic and Photothermal Phenomena. pp. 531–532, Berlin, Heidelberg. Springer Series in Optical Science Vol. 58. Series Editor A.L. Schawlow, Springer-Verlag
Eigen, M (1968) New looks and outlooks in physical enzymology. Quart Rev Biophys 1(1) pp 3–33
Fernyhough, P, Foyer, CH and Horton, P (1984) Increase in level of thylakoid protein phosphorylation in maize mesophyll chloroplasts by decrease in transthylakoid pH gradient. FEBS Lett 176: 133–138
Fisahn, J and Hansen, UP (1986) The influence of temperature on a K+-channel and on a carrier-type transporter in Nitella. J Exp Bot 177: 440–460
Geacintov, NE and Breton, J (1987) Energy transfer and fluorescence mechanisms in photosynthetic membranes. CRC Crit Rev in Plant Sci 5: 1–44
Hansen, UP (1982) Kinetic analysis of regulation of transport. In: Membranes and Transport: A Critical Review. Vol. 2, A.Martonosi (ed.) Plenum Press, New York and London pp 639–644
Hansen, UP (1985) Messung und reaktionskinetische Interpretation der Kinetik der Lichtwirkung auf den elektrophoretischen Transport über die Plasmamembran der Alge Nitella. Ber Deutsch Bot Ges 98: 105–118
Hansen, UP, Kolbowski, J and Dau, H (1987) Relationship between photosynthesis and plasmalemma transport. J Exp Bot 38: 1965–1981
Horton, P (1987) Interplay between environmental and metabolic factors in the regulation of electron transport in higher plants. In: Biggins, J (ed.) Progress in Photosynthetic Research, Vol. II. pp 683–688, Dordrecht: Martinus Nijhoff Publishers
Kolbowski, J (1987) Steady-state kinetics of chlorophyll-fluorescence and light-scattering. In: Biggins, J (ed.) Progress in Photosynthetic Research, Vol. II, pp 745–748, Dordrecht: Martinus Nijhoff Publishers
Kolbowski J (1988) Lineare Analyse der Signale aus dem photosynthetischen Apparat grüner Pflanzen mit besonderer Berücksichtigung der Wechselwirkung zwischen Photosynthese und Plasmalemmatransport. PhD-thesis, Kiel
Kolbowski, J, Keunecke, P, Thiel, M, Martens, J, Hansen, UP, Sanders, D and Slayman, CL (1985) Extended kinetics: Erweiterung eines Modells der Regelung des transmembranen Transportes in Nitella mit Hilfe gemeinsamer Zeitkonstanten in den Signalen von verschiedenen Ausgängen und ihrer Beeinflussung durch Gifte. Ber Deutsch Bot Ges 98: 119–129
Krause, GH (1973) The high-energy state of the thylakoid system as indicated by chlorophyll fluorescence and chloroplast shrinkage. Biochim Biophys Acta 292: 715–728
Krause, GH and Laasch, H (1987) Photoinhibition of photosynthesis. Studies on mechanisms of damage and protection in chloroplasts. In: Biggins, J (ed.) Progress in Photosynthetic Research, Vol. IV, pp 19–25, Dordrecht: Martinus Nijhoff Publishers
Krause, GH, Vernotte, C and Briantias, J-M (1982) Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Biochim Biophys Acta 679: 116–124
Krause, GH and Weis, E (1984) Chlorophyll-fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynth Res 5: 139–157
Laasch, H (1987) Non-photochemical quenching of chlorophyll a fluorescence in isolated chloroplasts under conditions of stressed photosynthesis. Planta 171: 220–226
Lavergne, J (1974) Fluorescence induction in algae and chloroplasts. Photochem Photobiol 20: 377–386
Martens, J, Hansen, UP and Warncke, J (1979) Further evidence for the parallel-pathways model obtained from the high-frequency slope of the light effect on the membrane potential in Nitella. J. Membrane Biol 48: 115–139
Melis, A and Schreiber, U (1979) The kinetic relationship between the C550 absorbance change, the reduction of Q(A320) and the variable fluorescence yield in chloroplasts at room temperature. Biochim Biophys Acta 547: 47–57
Oxborough, K and Horton, P (1987) Inhibition of high energy state in spinach chloroplasts by low concentrations of antimycin A. In: Biggins, J (ed.) Progress in Photosynthetic Research, Vol. II, pp 489–492, Dordrecht: Martinus Nijhoff Publishers
Poulet, P, Cahen, D and Malkin, S (1983) Photoacoustic detection of photosynthetic oxygen evolution from leaves. Quantative analysis by phase and amplitude measurements. Bichim Biophys Acta 724: 433–446
Quick, WP and Horton, P (1984) Studies on the induction of chlorophyll-fluorescence in barley. II. Resolution of fluorescence quenching by redox state and the transthylakoid pH gradient. Proc Roy Soc B 220: 371–382
Schreiber, U (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth Res 9: 261–272
Schreiber, U, Schliwa, U and Bilger, W (1986) Continuous recorded of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth Res 10: 51–62
Vanselow KH, Kolbowski J and Hansen UP (1988) Analysis of chlorophyll-fluorescence by means of noisy light. J Exp Bot (in press)
Weis, E and Berry, JA (1987) quantum efficiency of Photosystem 2 in relation to ‘energy’ dependent quenching of chlorophyll-fluorescence. Biochim Biophys Acta 894: 189–208
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Dau, H., Hansen, UP. Studies on the adaptation of intact leaves to changing light intensities by a kinetic analysis of chlorophyll fluorescence and oxygen evolution as measured by the photoacoustic signal. Photosynth Res 20, 59–83 (1989). https://doi.org/10.1007/BF00028622
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DOI: https://doi.org/10.1007/BF00028622