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Manipulation of rhizobia microflora for improving legume productivity and soil fertility: A critical assessment

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Abstract

Inputs of biologically fixed nitrogen derived from the symbiotic relationship between legumes and their root-nodule bacteria into terrestrial ecosystems amount to at least 70 million metric tons per year. It is obvious that this enormous quantity will need to be augmented as the world's population increases and as the natural resources that supply fertilizer nitrogen diminish. This objective will be achieved through the development of superior legume varieties, improvement in agronomic practice, and increased efficiency of the nitrogen fixation process itself by better management of the symbiotic relationship between plant and bacteria. This paper considers ways and means by which populations of root-nodule bacteria, established and introduced, can be manipulated ecologically, agronomically, edaphically and genetically to improve legume productivity and, as a consequence, soil fertility.

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References

  • Abbott L K and Robson A D 1982 The role of vesicular-arbuscular mycorrhiza in agriculture and the selection of fungi for inoculation. Aust. J. Agric. Res. 33, 389–408.

    Google Scholar 

  • Abbott L K and Robson A D 1987 Managing VA mycorrhizal fungi in pastures. In Proc. 7th NACOM Conference. Eds. D M Sylvia, L L Hung and J H Graham. pp 10–12. Inst. Forest Agric. Sci., Florida.

    Google Scholar 

  • Adebayo A, Watanabe I and Ladha J K 1989 Epiphytic occurrence of Azorhizobium caulinodans and other rhizobia on host and nonhost legumes. Appl. Environ. Microbiol. 55, 2407–2409.

    Google Scholar 

  • Alazard D and Duhoux E 1987 Diversity of stem nodulation sites in Aeschynomene spp. J. Plant Physiol. 132, 123–125.

    Google Scholar 

  • Alazard D, Ndoye I and Dreyfus B 1988 Sesbania rostrata and other stem-nodulated legumes. In Nitrogen Fixation: Hundred Years After. Eds. H Bothe, F Jde Bruijn and W E Newton. pp 765–769. Gustav Fischer, Stuttgart.

    Google Scholar 

  • Albrecht S L, Bennett J M and Boote K J 1984 Relationship of nitrogenase activity to plant water stress in field-grown soybeans. Field Crops Res. 8, 61–71.

    Article  Google Scholar 

  • Allen E K and Allen O N 1961 The scope of nodulation in the Leguminosae. In Recent Advances in Botany. Vol. 1. Proc. 9th Int. Bot. Congr. pp 585–588. Univ. Toronto Press, Toronto.

    Google Scholar 

  • Allen O N and Allen E K 1981 The Leguminosae. A Source Book of Characteristics, Uses and Nodulation. Univ. Wisconsin Press, Madison, WI. 812p.

    Google Scholar 

  • Almedras A S and Bottomley P J 1987 Influence of lime and phosphate on nodulation of soil-grown Trifolium subterraneum L. by indigenous Rhizobium trifolii. Appl. Environ. Microbiol. 55, 2090–2097.

    Google Scholar 

  • Almendras A S and Bottomley P J 1988 Cation and phosphate influences on the nodulating characteristics of indigenous serogroups of Rhizobium trifolii on soil grown Trifolium subterraneum. Soil Biol. Biochem. 20, 345–351.

    Article  Google Scholar 

  • Amarger N 1981 Competition for nodule formation between effective and ineffective strains of Rhizobium meliloti. Soil Biol. Biochem. 13, 475–480.

    Article  Google Scholar 

  • Anderson A J 1970 Trace elements for sheep pastures and fodder crops in Australia. J. Aust. Inst. Agric. Sci. 36, 15–29.

    Google Scholar 

  • Andrew C S 1976 Effect of calcium, pH and nitrogen on the growth and chemical composition of some tropical and temperate pasture legumes. 1. Nodulation and growth. Aust. J. Agric. Res. 27, 611–623.

    Google Scholar 

  • Asai T 1944 Uber die Mykorrhizenbildung der leguminosen pflanzen. Jap. J. Bot. 13, 463–484.

    Google Scholar 

  • Atkin-Smith R E, Gault R R and Brockwell J 1986 Effective rhizobia can be introduced, by surface application, into poorly-nodulated subterranean clover pasture. In Proc. 8th Aust. Nitrogen Fixation Conference. AIAS Occ. Publ. No. 25. Eds. W Wallace and S E Smith. pp 151–152. Aust. Inst. Agric. Sci., Adelaide.

    Google Scholar 

  • Ausubel F M, Buikema W J, Earl D C, Klingensmith J A, Nixon B and Szeto W W 1985 Organization and regulation of Rhizobium meliloti and Parasponia Bradyrhizobium nitrogen fixation genes. In Nitrogen Fixation Research Progress. Eds. H J Evans, P J Bottomley and W E Newton. pp 165–171. Martinus Nijhoff, Dordrecht.

    Google Scholar 

  • Badr El-Din S M S and Moawad H 1988 Enhancement of nitrogen fixation in lentil, faba bean, and soybean by dual inoculation with Rhizobia and mycorrhizae. Plant and Soil 108, 117–124.

    Google Scholar 

  • Barea J M and Azcon-Aguilar C 1983 Mycorrhizas and their significance in nodulating nitrogen-fixing plants. Adv. Agron. 36, 1–54.

    Google Scholar 

  • Barkdoll A W, Sartain J B and Hubbell D H 1983 Effect of soil implanted granular and pellet Rhizobium inoculant in Phaseolus vulgaris L. in Honduras. Soil Crop Sci. Soc. Fla. Proc. 42, 184–189.

    Google Scholar 

  • Basit H A, Angle J S, Salem S and Gewailly E M 1992 Phage coating of soybean seed reduces nodulation by indigenous soil bradyrhizobia. Can. J. Microbiol. 38, 1264–1269.

    Google Scholar 

  • Bassam B J, Rolfe B G and Djordjevic M A 1986 Macroptilium atropurpureum (siratro) host specificity genes are linked to a nodD-like gene in the broad host range Rhizobium strain NGR234. Mol. Gen. Genet. 203, 49–57.

    Article  Google Scholar 

  • Becker M, Ladha J K and Ali M 1995 Green manure technology: Potential, usage and limitations-a case history for lowland rice. Plant and Soil 174.

  • Becker M, Ladha J K and Ottow J C 1990 Growth and N2 fixation of two stem-nodulating legumes and their effect as green manure on lowland rice. Soil Biol. Biochem. 22, 1109–1119.

    Article  Google Scholar 

  • Bell F and Nutman P S 1971 Experiments on nitrogen fixation by nodulated lucerne. Plant and Soil, special vol., 231–264.

  • Berg R KJr, Loynachan T E, Zablotowicz R M and Lieberman M T 1988 Nodule occupancy by introduced Bradyrhizobium japonicum in Iowa soils. Agron. J. 80, 876–881.

    Google Scholar 

  • Bergersen F J (Ed.) 1980 Methods for Evaluating Biological Nitrogen Fixation. John Wiley and Sons, Chichester, UK. 702p.

    Google Scholar 

  • Bergersen F J, Turner G L, Chase D L, Gault R R and Brockwell J 1985 The natural abundance of 15N in an irrigated soybean crop and its use for the calculation of nitrogen fixation. Aust. J. Agric. Res. 36, 411–423.

    Google Scholar 

  • Bonish P M 1979 Clover rhizobia in soils: assessment of effectiveness using the plant infection count method. N. Z. J. Agric. Res. 22, 89–93.

    Google Scholar 

  • Boonkerd N, Arunsri C, Rungrattanakasin W and Vasurat Y 1984 Effect of postemergence inoculation on field grown soybeans. In Advances in Nitrogen Fixation Research. Eds. C Veeger and W E Newton. p 327. Martinus Nijhoff/Dr. Junk Publ., The Hague.

    Google Scholar 

  • Boonkerd N and Weaver R W 1982 Survival of cowpea rhizobia in soil as affected by soil temperature and moisture. Appl. Environ. Microbiol. 43, 585–589.

    Google Scholar 

  • Bosworth A H, Williams M K, Albrecht K A, Kwiatkowski R K, Beynon J, Hankinson T R, Ronson C W, Cannon F, Wacek T C and Triplett E W 1994 Alfalfa yield response in inoculation with recombinant strains of Rhizobium meliloti with an extra copy of dctABD and/or modified nifA expression. Appl. Environ. Microbiol. 60, 3815–3832.

    PubMed  Google Scholar 

  • Bottomley P J 1992 Ecology of Bradyrhizobium and Rhizobium. In Biological Nitrogen Fixation. Eds. G Stacey, R H Burris and H J Evans. pp 293–348. Chapman and Hall, New York.

    Google Scholar 

  • Bottomley P J, Cheng H-H and Strain S R 1994 Genetic structure and symbiotic characteristics of a Bradyrhizobium population recovered from a pasture soil. Appl. Environ. Microbiol. 60, 1754–1761.

    Google Scholar 

  • Bottomley P J and Jenkins M B 1983 Some characteristics of Rhizobium meliloti isolates from alfalfa fields in Oregon. Soil Sci. Soc. Am. J. 47, 1153–1157.

    Google Scholar 

  • Bounejmate M and Robson A D 1992 Differential tolerance of genotypes of Medicago truncatula to low pH. Aust. J. Agric. Res. 43, 731–737.

    Google Scholar 

  • Bowen G D 1980 Misconceptions, concepts and approaches in rhizosphere biology. In Contemporary Microbial Ecology. Eds. D C Ellwood, J N Hedger, M J Latham, J M Lynch and J H Slater. pp 283–305. Academic Press, London.

    Google Scholar 

  • Bowen G D and Kennedy M 1959 Effect of high soil temperature on Rhizobium spp. Q. J. Agric. Sci. 16, 177–197.

    Google Scholar 

  • Brewin N J, Wood E A and Young J P W 1983 Contribution of the symbiotic plasmid to the competitiveness of Rhizobium leguminosarum. J. Gen. Microbiol. 129, 2973–2977.

    Google Scholar 

  • Brockwell J 1962 Studies on seed pelleting as an aid to legume seed inoculation. 1. Coating materials, adhesives, and methods of inoculation. Aust. J. Agric. Res. 13, 638–649.

    Google Scholar 

  • Brockwell J 1963 Accuracy of a plant-infection test for counting populations of Rhizobium trifolii. Appl. Microbiol. 11, 377–383.

    Google Scholar 

  • Brockwell J 1971 An appraisal of an IBP experiment on nitrogen fixation by nodulated legumes. Plant and Soil, special vol., 265–272.

  • Brockwell J 1977 Application of legume seed inoculants. In A Treatise on Dinitrogen Fixation. Section IV. Agronomy and Ecology. Eds. R W F Hardy and A H Gibson. pp 277–309. John Wiley and Sons, New York.

    Google Scholar 

  • Brockwell J 1982 Inoculation methods for field experimenters and farmers. In Nitrogen Fixation in Legumes. Ed. J M Vincent. pp 211–227. Academic Press, Sydney.

    Google Scholar 

  • Brockwell J and Bottomley P J 1995 Recent advances in inoculant technology and prospects for the future. Soil Biol. Biochem. 27 (In press).

  • Brockwell J, Diatloff A, Roughley R J and Date R A 1982 Selection of rhizobia for inoculants. In Nitrogen Fixation in Legumes. Ed. J M Vincent. pp 173–191. Academic Press, Sydney.

    Google Scholar 

  • Brockwell J, Gault R R and Chase D L 1977 Inoculating soybeans with root-nodule bacteria. Farmers' Newsl. 104, 7–12. Irrigation Research and Extension Committee, Griffith, NSW.

    Google Scholar 

  • Brockwell J, Gault R R, Chase D L, Hely F W, Zorin M and Corbin E J 1980 An appraisal of practical alternatives to legume seed inoculation: field experiments on seed bed inoculation with solid and liquid inoculants. Aust. J. Agric. Res. 31, 47–60.

    Google Scholar 

  • Brockwell J, Gault R R, Chase D L, Turner G L and Bergersen F J 1985 Establishment and expression of soybean symbiosis in a soil previously free of Rhizobium japonicum. Aust. J. Agric. Res. 36, 397–409.

    Google Scholar 

  • Brockwell J and Hely F W 1962 Some aspects of the ecology of Rhizobium meliloti in the brown acid soils of the Macquarie Region of New South Wales. Fld Stn Rec., Div. Plant Ind., CSIRO (Aust.) 1(2), 1–11.

    Google Scholar 

  • Brockwell J and Hely F W 1966 Symbiotic characteristics of Rhizobium meliloti: an appraisal of the systematic treatment of nodulation and nitrogen fixation interactions between hosts and rhizobia of diverse origins. Aust. J. Agric. Res. 17, 885–899.

    Google Scholar 

  • Brockwell J, Herridge D F, Roughley R J, Thompson J A and Gault R R 1975 Studies on seed pelleting as an aid to legume seed inoculation. 4. Examination of preinoculated seed. Aust. J. Exp. Agric. Anim. Husb. 15, 780–787.

    Google Scholar 

  • Brockwell J, Holliday R A and Pilka A 1988 Evaluation of the symbiotic nitrogen-fixing potential of soils by direct microbiological means. Plant and Soil 108, 163–170.

    Google Scholar 

  • Brockwell J and Katznelson J 1976 Symbiotic characteristics of Rhizobium trifolii from Israel in association with ten species of Trifolium. Aust. J. Agric. Res. 27, 799–810.

    Google Scholar 

  • Brockwell J and Robinson A C 1970 Observations on the natural distribution of Rhizobium spp. relative to physical features of the landscape. Proc. 11th Int. Grassl. Congr. pp 438–441. Univ. Queensland Press, Brisbane, Australia.

    Google Scholar 

  • Brockwell J and Roughley R J 1967 An examination of the numbers of nodule bacteria associated with legume seed following commercial multiple inoculation. J. Aust. Inst. Agric. Sci. 33, 204–207.

    Google Scholar 

  • Brockwell J, Roughley R J and Herridge D F 1987 Population dynamics of Rhizobium japonicum strains used to inoculate three successive crops of soybean. Aust. J. Agric. Res. 38, 61–74.

    Google Scholar 

  • Bromfield E S P 1984 The preference for strains of Rhizobium meliloti by cultivars of Medicago sativa grown on agar. Can. J. Microbiol. 30, 1179–1183.

    Google Scholar 

  • Bromfield E S P and Jones D G 1980 Studies on acid tolerance of Rhizobium trifolii in culture and in soil. J. Appl. Bacteriol. 48, 253–264.

    Google Scholar 

  • Bromfield E S P, Wheatcroft R and Barran L R 1994 Medium for direct isolation of Rhizobium meliloti from soil. Soil Biol. Biochem. 26, 423–428.

    Article  Google Scholar 

  • Bromfield S M, Cumming R W, David D J and Williams C H 1983 Change in soil pH, manganese and aluminium under subterranean clover pasture. Aust. J. Exp. Agric. Anim. Husb. 23, 181–191.

    Google Scholar 

  • Broughton W J, Samrey U and Stanley J 1987 Ecological genetics of Rhizobium meliloti symbiotic plasmid transfer in the Medicago sativa rhizosphere. FEMS Microbiol. Lett. 40, 251–255.

    Article  Google Scholar 

  • Broughton W J, Wong C H, Lewin A, Samrey U, Myint H, Meyer H, Dowling D N and Simon R 1986 Identification of Rhizobium plasmid sequences involved in recognition of Psophocarpus, Vigna, and other legumes. J. Cell Biol. 102, 1173–1182.

    Article  PubMed  Google Scholar 

  • Brown M R, Wolf D D, Morse R D and Neal J L 1983 Viability of Rhizobium in fertilizer slurries used for hydroseeding. J. Environ. Qual. 12, 388–390.

    Google Scholar 

  • Bunt J S 1988 Nitrogen fixation in the sea. In Microbiology in Action. Eds. W G Murrell and I R Kennedy. pp 209–219 Research Studies Press, Letchworth, UK.

    Google Scholar 

  • Burns R C and Hardy R W F 1975 Nitrogen Fixation in Bacteria and Higher Plants. Springer Verlag, New York. 189p.

    Google Scholar 

  • Burton J C 1976 Methods of inoculating seeds and their effect on survival of rhizobia. In Symbiotic Nitrogen Fixation in Plants. International Biological Programme No. 7. Ed. P S Nutman. pp 175–189. Univ. Press, Cambridge, UK.

    Google Scholar 

  • Burton J C 1979 Rhizobium species. In Microbial Technology, 2nd ed., Vol. 1. Eds. H J Peppler and D Perlman. pp 29–58. Academic Press, New York.

    Google Scholar 

  • Burton J C 1982 Modern concepts in legume inoculation. In Biological Nitrogen Fixation Technology for Tropical Agriculture. Eds. P H Graham and S C Harris. pp 105–114. CIAT, Cali, Colombia.

    Google Scholar 

  • Bushby H V A 1981 Quantitative estimation of rhizobia in nonsterile soil using antibodies and fungicides. Soil Biol. Biochem. 13, 237–239.

    Article  Google Scholar 

  • Bushby H V A 1982 Ecology. In Nitrogen Fixation. Volume 2: Rhizobium. Ed. W J Broughton. pp 35–75. Clarendon Press, Oxford.

    Google Scholar 

  • Bushby H V A 1984 Colonization of rhizospheres and nodulation of two Vigna species by rhizobia inoculated onto seed: influence of soil. Soil Biol. Biochem. 16, 635–641.

    Article  Google Scholar 

  • Bushby H V A and Marshall K C 1977 Water status of rhizobia in relation to their susceptibility to desiccation and to their protection by montmorillonite. J. Gen. Microbiol. 99, 19–27.

    Google Scholar 

  • Caldwell B E and Vest G 1970 Effects of Rhizobium japonicum strains on soybean yield. Crop Sci. 10, 19–21.

    Google Scholar 

  • Carroll B J, McNeil D L and Gresshoff P M 1985 A supernodulation and nitrate-tolerant symbiotic (nts) soybean mutant. Plant Physiol. 78, 34–40.

    Google Scholar 

  • Cassman K G, Singleton P W and Linquist B A 1993 Input/output analysis of the cumulative soybean response to phosphorus on an Ultisol. Field Crops Res. 34, 23–36.

    Article  Google Scholar 

  • Cassman K G, Munns D N and Beck D P 1981 Growth of Rhizobium strains at low concentrations of phosphate. Soil Sci. Soc. Am. J. 45, 520–523.

    Google Scholar 

  • Chamber M A 1983 Influence of several methods for rhizobial inoculation on nodulation and yield of soybeans. Plant and Soil 74, 203–209.

    Google Scholar 

  • Chanaseni C and Kongngoen S 1992 Extension programs to promote rhizobial inoculants for soybean and groundnut in Thailand. Can. J. Microbiol. 38, 594–597.

    Google Scholar 

  • Chatel D L, Greenwood R M and Parker C A 1968 Saprophytic competence as an important character in the selection of Rhizobium for inoculation. Trans. 9th Int. Congr. Soil Sci., Vol 2. pp 65–73. Augus and Robertson, Sydney, Australia.

    Google Scholar 

  • Chen Y P, Glenn A R and Dilworth M J 1984 Uptake and oxidation of aromatic substrates by Rhizobium leguminosarum MNF3841 and Rhizobium trifolii TA1. FEMS Microbiol. Lett. 21, 201–205.

    Article  Google Scholar 

  • Ciafardini G and Barbieri C 1987 Effects of cover inoculation of soybean on nodulation, nitrogen fixation, and yield. Agron. J. 79, 645–648.

    Google Scholar 

  • Cocks P S, Mathison M J and Crawford E J 1980 From wild plants to pasture cultivars: annual medics and subterranean clover in southern Australia. In Advances in Legume Science. Eds. R J Summerfield and A H Bunting. pp 569–596. Royal Botanic Gardens, Kew.

    Google Scholar 

  • Corbin E J, Brockwell J and Gault R R 1977 Nodulation studies on chickpea (Cicer arietinum). Aust. J. Exp. Agric. Anim. Husb. 17, 126–134.

    Google Scholar 

  • Coventry D R, Hirth J R, Reeves T G and Burnett V F 1983a Growth and nitrogen fixation by subterranean clover in response to inoculation, molybdenum application and soil amendment with lime. Soil Biol. Biochem. 17, 791–796.

    Article  Google Scholar 

  • Coventry D R, Hirth J R, Reeves T G and Jones H R 1983b Development of populations of Rhizobium trifolii and nodulation of subterranean clover following the cropping phase in crop-pasture rotations in southern Australia. Soil Biol. Biochem. 17, 17–22.

    Article  Google Scholar 

  • Cregan P B and Keyser H H 1988 Influence of Glycine spp. on competitiveness of Bradyrhizobium japonicum and Rhizobium fredii. Appl. Environ. Microbiol. 54, 803–808.

    Google Scholar 

  • Cunningham S, Kollmeyer W D and Stacey G 1991 Chemical control of interstrain competition for soybean nodulation by Bradyrhizobium japonicum. Appl. Environ. Microbiol. 57, 1886–1892.

    PubMed  Google Scholar 

  • Danso S K A, Kapuya J and Hardarson G 1990a Nitrogen fixation and growth of soybean as influenced by varying the methods of inoculation with Bradyrhizobium japonicum. Plant and Soil 125, 81–86.

    Google Scholar 

  • Danso S K A, Zapata F and Awonaike K O 1990b Effect of postemergence, supplemental inoculation on nodulation and symbiotic performance of soybean (Glycine max (L.) Merrill) at three levels of soil nitrogen. Appl. Environ. Microbiol. 56, 1793–1798.

    Google Scholar 

  • Date R A 1969 A decade of legume inoculant control in Australia. J. Aust. Inst. Agric. Sci. 35, 27–37.

    Google Scholar 

  • Date R A 1977 Inoculation of tropical pasture legumes. In Exploiting the Legume-Rhizobium Syrnbiosis in Tropical Agriculture. Eds. J M Vincent, A S Whitney and J Bose. pp 293–311. Univ. Hawaii, Honolulu.

    Google Scholar 

  • Data R A 1982 Collection, isolation, characterization and conservation of Rhizobium. In Nitrogen Fixation in Legumes. Ed. J M Vincent. pp 95–109. Academic Press, Sydney.

    Google Scholar 

  • Date R A and Decker A M 1965 Minimal antigenic constitution of 28 strains of Rhizobium japonicum. Can. J. Microbiol. 11, 1–8.

    PubMed  Google Scholar 

  • Date R A and Halliday J 1979 Selecting Rhizobium for acid, infertile soils of the tropics. Nature, London 277, 62–64.

    Google Scholar 

  • Date R A and Roughley R J 1977 Preparation of legume seed inoculants. In A Treatise on Dinitrogen Fixation. Section IV. Agronomy and Ecology. Eds. R W F Hardy and A H Gibson. pp 243–275. John Wiley and Sons, New York.

    Google Scholar 

  • Dazzo F B, Hrabek E M, Urbano M R, Sherwood J E and Truchet G 1981 Regulation of recognition in the Rhizobium-clover symbiosis. In Current Perspectives in Nitrogen Fixation. Eds. A H Gibson and W E Newton. pp 292–295. Aust. Acad. Sci., Canberra.

    Google Scholar 

  • Demezas D H and Bottomley P J 1986a Autecology in rhizospheres and nodulating behaviour of indigenous Rhizobium trifolii. Appl. Environ. Microbiol. 52, 1014–1019.

    Google Scholar 

  • Demezas D H and Bottomley P J 1986b Interstrain competition between representatives of indigenous serotypes of Rhizobium trifolii. Appl. Environ. Microbiol. 52, 1020–1025.

    Google Scholar 

  • Demezas D H and Bottomley P J 1987 Influence of soil and nonsoil environments on nodulation by Rhizobium trifolii. Appl. Environ. Microbiol. 53, 596–597.

    Google Scholar 

  • Demezas D H, Reardon T B, Watson J M and Gibson A H 1991 Genetic diversity among Rhizobium leguminosarum bv. Trifolii strains revealed by allozyme and restriction fragment length polymorphism analyses. Appl. Environ. Microbiol. 57, 3489–3495.

    Google Scholar 

  • Diatloff A and Brockwell J 1976 Ecological studies of root-nodule bacteria introduced into field environments. 4. Symbiotic properties of Rhizobium japonicum and competitive success in nodulation of two Glycine max cultivars by effective and ineffective strains. Aust. J. Exp. Agric. Anim. Husb. 16, 514–521.

    Google Scholar 

  • Dilworth M J, McKay I, Franklin M and Glenn A R 1983 Catabolite effects on enzyme induction and substrate utilization in Rhizobium leguminosarum. J. Gen. Microbiol. 129, 359–366.

    Google Scholar 

  • Djordjevic M A, Redmond J W, Batley M and Rolfe B G 1987 Clovers secrete specific phenolic compounds which either stimulate or repress nod gene expression in Rhizobium trifolii. EMBO J. 6, 1173–1179.

    Google Scholar 

  • Djordjevic M A, Zurkowski W and Rolfe B G 1982 Plasmids and stability of symbiotic properties of Rhizobium trifolii. J. Bacteriol. 151, 560–568.

    PubMed  Google Scholar 

  • Dommergues Y R 1987 The role of biological nitrogen fixation in agroforestry. In Agroforestry: A Decade of Development. Eds. H A Steppler and P K R Nair. pp 245–271. International Council for Research in Agroforestry, Nairobi.

    Google Scholar 

  • Dommergues Y R, Diem H G and Divies C 1979 Polyacrylamideentrapped Rhizobium as an inoculant for legumes. Appl. Environ. Microbiol. 37, 779–781.

    Google Scholar 

  • Donald C M and Williams C H 1954 Fertility and productivity of a podzolic soil as influenced by subterranean clover (Trifolium subterranean L.) and superphosphate. Aust. J. Agric. Res. 5, 664–687.

    Google Scholar 

  • Dowdle S F and Bohlool B B 1985 Predominance of fast-growing Rhizobium japonicum in a soybean field in the People's Republic of China. Appl. Environ. Microbiol. 54, 1171–1176.

    Google Scholar 

  • Dowling D N and Broughton W J 1986 Competition for nodulation of legumes. Annu. Rev. Microbiol. 40, 131–157.

    Article  PubMed  Google Scholar 

  • Downey J and van Kessel C 1990 Dual inoculation of Pisum sativum with Rhizobium leguminosarum and Penicillium bilaji. Biol. Fertil. Soils 10, 194–196.

    Google Scholar 

  • Dreyfus B, Garcia J L and Gillis M 1988 Characterization of Azorhizobium caulinodans gen. nov., sp. nov., a stem-nodulating nitrogen-fixing bacteria isolated from Sesbania rostrata. Int. J. Syst. Bacteriol. 38, 89–98.

    Google Scholar 

  • Dreyfus B L and Dommergues Y R 1981a Nitrogen-fixing nodules induced by Rhizobium on the stem of the tropical legume Sesbania rostrata. FEMS Microbiol. Lett. 10, 313–317.

    Article  Google Scholar 

  • Dreyfus B L and Dommergues Y R 1981b Nodulation of Acacia species by fast- and slow- growing tropical strains of Rhizobium. Appl. Environ. Microbiol. 41, 97–99.

    Google Scholar 

  • Duc G and Messager A 1989 Mutagenesis of pea (Pisum sativum L.) and the isolation of mutants for nodulation and nitrogen fixation. Plant Sci. 60, 207–213.

    Article  Google Scholar 

  • Dudman W F and Brockwell J 1968 Ecological studies of root-nodule bacteria introduced into field environments. I. A survey of field performance of clover inoculants by gel immune diffusion serology. Aust. J. Agric. Res. 19, 739–747.

    Google Scholar 

  • Dughri M H and Bottomley P J 1983 Effect of acidity on the composition of an indigenous soil population of Rhizobium trifolii found in nodules of Trifolium subterraneum L. Appl. Environ. Microbiol. 46, 1207–1213.

    Google Scholar 

  • Dughri M H and Bottomley P J 1984 Soil acidity and the composition of an indigenous population of Rhizobium trifolii in nodules of different cultivars of Trifolium subterraneum L. Soil Biol. Biochem. 16, 405–411.

    Article  Google Scholar 

  • Eaglesham A R J, Ellis J M, Evans W R, Fleishman D E, Hungria M and Hardy R W F 1990 The first photosynthetic N2-fixing Rhizobium: characteristics. In Nitrogen Fixation: Achievements and Objectives. Eds. P M Gresshoff, L E Roth, G Stacey and W E Newton. pp 805–811. Chapman and Hall, New York.

    Google Scholar 

  • Eardly B D, Materon L A, Smith N H, Johnson D A, Rumbaugh M D and Selander R K 1990 Genetic structure of natural populations of the nitrogen-fixing bacterium Rhizobium meliloti. Appl. Environ. Microbiol. 56, 187–194.

    PubMed  Google Scholar 

  • Elegba M S and Rennie R J 1984 Effect of different inoculant adhesive agents on rhizobial survival, nodulation, and nitrogenase (acetylene-reducing) activity of soybeans (Glycine max (L.) Merill). Can. J. Soil Sci. 64, 631–636.

    Google Scholar 

  • Evans H J and Russell S A 1971 Physiological chemistry of symbiotic nitrogen fixation by legumes. In Chemistry and Biochemistry of Nitrogen Fixation. Ed. J R Postgate. pp 191–244. Plenum Press, London.

    Google Scholar 

  • Evans H J, Hanus F J, Haugland R A, Cantrell M A, Xu L-S, Russell S A, Lambert G R and Harker A R 1985 Hydrogen recycling in nodules affects nitrogen fixation and growth of soybeans. In World Soybean Research Conference III. Proceedings. Ed. R Shibles. pp 935–942. Westcourt Press, Boulder, CO.

    Google Scholar 

  • Firmin J L, Wilson K E, Rossen L and Johnston A W B 1986 Flavonoid activation of nodulation genes in Rhizobium reversed by other compounds present in plants. Nature, London 324, 90–92.

    Google Scholar 

  • Fobert P R, Roy N, Nash J H E and lyer V N 1991 Procedure for obtaining efficient root nodulation of a pea cultivar by a desired Rhizobium strain and preempting nodulation by other strains. Appl. Environ. Microbiol. 57, 1590–1594.

    Google Scholar 

  • Franssen H J, Vijn I, Yang W C and Bisseling T 1992 Developmental aspects of the Rhizobium-legume symbiosis. Plant Mol. Biol. 19, 89–107.

    Article  PubMed  Google Scholar 

  • Fred E B, Baldwin I L and McCoy E 1932 Root Nodule Bacteria and Leguminous Plants. Stud. Sci. Univ. Wisconsin No. 5. 343p.

  • Fuhrmann J, Davey C B and Wollum A GII 1986 Desiccation tolerance of clover rhizobia in sterile soils. Soil Sci. Soc. Am. J. 50, 639–644.

    Google Scholar 

  • Gallacher A E and Sprent J I 1978 The effect of different water regimes on growth and nodule development ofgreenhouse grown V. faba. J. Exp. Bot. 29, 413–423.

    Google Scholar 

  • Gault R R 1978 A study of developments and trends, in New Zealand, the U.S.A., and Canada, in the technology associated with the exploitation of the nitrogen-fixing legume root nodule bacteria, Rhizobium spp., for use in legume crops new to Australian agriculture. Final Report to the Winston Churchill Memorial Trust. CSIRO, Canberra. 143 p.

    Google Scholar 

  • Gault R R, Banks L W, Chase D L and Brockwell J 1984 Remedial measures to salvage unnodulated soybean crops. J. Aust. Inst. Agric. Sci. 50, 244–246.

    Google Scholar 

  • Gault R R, Bernardi A L, Thompson J A, Andrews J A, Banks L W, Hebb D M and Brockwell J 1994a Studies on alternative means of legume inoculation: appraisal of application of inoculant suspended in irrigation water (water-run inoculation). Aust. J. Exp. Agric. 34, 401–409.

    Google Scholar 

  • Gault R R and Brockwell J 1980 Studies on seed pelleting as an aid to legume inoculation. 5. Effects of incorporation of molybdenum compounds in the seed pellet on inoculant survival, seedling nodulation and plant growth of lucerne and subterranean clover. Aust. J. Exp. Agric. Anim. Husb. 20, 63–70.

    Google Scholar 

  • Gault R R and Brockwell J 1988 The autecology of a strain of Bradyrhizobium japonicum in a soybean rice rotation. Proc. Sth Aust. Soybean Research Workshop. pp 82–84. NSW Agriculture and Fisheries, Sydney.

    Google Scholar 

  • Gault R R, Pilka A, Hebb D M and Brockwell J 1994b Nodulation studies on legumes exotic to Australia: symbiotic relationships between Chamaecytisus palmensis (tagasaste) and Lotus spp. Aust. J. Exp. Agric. 35, 385–394.

    Google Scholar 

  • Gault R R and Schwinghamer E A 1993 Direct isolation of Bradyrhizobium japonicum from soil. Soil Biol. Biochem. 25, 1161–1166.

    Article  Google Scholar 

  • Gaworzewska E T and Carlile M J 1982 Positve chemotaxis of Rhizobium leguminosarum and other bacteria towards root exudates from legumes and other plants. J. Gen. Microbiol. 128, 1179–1188.

    Google Scholar 

  • Gemell L G and Roughley R J 1993 Field evaluation in acid soils of Rhizobium legurninosarum b.v. trifolii selected for their tolerance or sensitivity to acid soil factors in agar medium. Soil Biol. Biochem. 25, 1447–1452.

    Article  Google Scholar 

  • George T, Ladha J K, Buresh R J and Garrity D P 1992 Managing native and legume-fixed nitrogen in lowland rice-based cropping systems. Plant and Soil 141, 69–91.

    Google Scholar 

  • Gibson A H 1963 Physical environment and symbiotic nitrogen fixation. I. The effect of root temperature on recently nodulated Trifolium subterraneum L. plants. Aust. J. Biol. Sci. 16, 28–42.

    Google Scholar 

  • Gibson A H 1964 Genetic control of strain-specific ineffective nodulation in Trifolium subterraneum L. Aust. J. Agric. Res. 15, 37–49.

    Google Scholar 

  • Gibson A H 1967 Physical environment and symbiotic nitrogen fixation. IV. Factors affecting the early stages of nodulation. Aust. J. Biol. Sci. 20, 1087–1104.

    Google Scholar 

  • Gibson A H, Curnow B C, Bergersen F J, Brockwell J and Robinson A C 1975 Studies of field populations of Rhizobium: effectiveness of strains of Rhizobium trifolii associated with Trifolium subterraneum L. pastures in south-eastern Australia. Soil Biol. Biochem. 7, 95–102.

    Article  Google Scholar 

  • Gibson A H, Demezas D H, Gault R R, Bhuvaneswari T V and Brockwell J 1990 Genetic stability in rhizobia in the field. Plant and Soil 129, 37–44.

    Google Scholar 

  • Gibson A H, Dudman W F, Weaver R W, Horton J C and Anderson I C 1971 Variation within serogroup 123 of Rhizobium japonicum. Plant and Soil, special vol., 33–37.

  • Glenn A R and Dilworth M J 1981 Oxidation of substrates by isolated bacteroids and free living cells of Rhizobium leguminosarum 3841. J. Gen. Microbiol. 126, 243–247.

    Google Scholar 

  • Graham P H 1964 The application of computer techniques to the taxonomy of the root-nodule bacteria of legumes. J. Gen. Microbiol. 35, 511–517.

    Google Scholar 

  • Graham P H 1969 Selective medium for growth of Rhizobium. Appl. Microbiol. 17, 769–770.

    PubMed  Google Scholar 

  • Graham P H, Sadowsky M J, Keyser H H, Barnet Y M, Bradley R S, Cooper J E, De Ley D J, Jarvis B D W, Roslycky E B, Strijdom B W and Young J P W 1991 Proposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria. Int. J. Syst. Bacteriol. 41, 582–587.

    Google Scholar 

  • Graham P H, Viteri S E, Mackie F, Vargas A T and Palacios A 1982 Variation in acid soil tolerance among strains of Rhizobium phaseoli. Field Crops Res. 5, 121–128.

    Article  Google Scholar 

  • Graham-Weiss L, Bennett M L and Paau A S 1987 Production of bacterial inoculants by direct fermentation on nutrient-supplemented vermicilite. Appl. Environ. Microbiol. 53, 2138–2140.

    Google Scholar 

  • Gremaud M F and Harper J E 1989 Selection and initial characterization of partially nitrate tolerant mutants of soybean. Plant Physiol. 89, 169–173.

    Google Scholar 

  • Griffith G W and Roughley R J 1992 The effect of moisture potential on growth and survival of root nodule bacteria in peat culture and on seed. J. Appl. Bacteriol. 73, 1–13.

    PubMed  Google Scholar 

  • Griffith G W, Roughley R J and Brown J F 1992 The effect of packaging films on growth and survival of rhizobia in peat culture. Lett. Appl. Microbiol. 14, 241–243.

    Google Scholar 

  • Hagedorn C, Ardahl A H and Materon L A 1983 Characteristics of Rhizobium trifolii populations associated with subclover in Mississippi soils. Soil Sci. Soc. Am. J. 47, 1148–1152.

    Google Scholar 

  • Hale C N 1981 Methods of white clover inoculation-their effect on competition for nodule formation between naturalised and inoculated strains of Rhizobium trifolii. N.Z. J. Exp. Agric. 9, 169–172.

    Google Scholar 

  • Ham G E 1980 Inoculation of legumes with Rhizobium in competition with naturalized strains. In Nitrogen Fixation, Vol. 2. Eds. W E Newton and W H Orme-Johnson. pp 131–138. Univ. Park Press, Baltimore.

    Google Scholar 

  • Ham G E, Frederick L R and Anderson I C 1971 Serogroups of Rhizobium japonicum in soybean nodules sampled in Iowa. Agron. J. 63, 69–72.

    Google Scholar 

  • Harris J R 1954 Rhizosphere relationships of subterranean clover. Aust. J. Agric. Res. 5, 247–270.

    Google Scholar 

  • Harrison S P, Mytton L R, Skot L, Dye M and Cresswell A 1992 Characterisation of Rhizobium isolates by amplification of DNA polymorphisms using random primers. Can. J. Microbiol. 38, 1009–1015.

    PubMed  Google Scholar 

  • Hartel P G and Alexander M 1983 Growth and survival of cowpea rhizobia in acid, aluminium-rich soils. Soil Sci. Soc. Am. J. 47, 502–506.

    Google Scholar 

  • Hartley R A, Lawn R J and Byth D E 1993 Genotypic variation in growth and seed yield of soybean [Glycine max (L.) Merr.] in saturated soil culture. Aust. J. Agric. Res. 44, 689–702.

    Google Scholar 

  • Hashem F M and Angle J S 1988 Rhizobiophage effects on Bradyrhizobium japonicum nodulation and soybean growth. Soil Biol. Biochem. 20, 69–73.

    Article  Google Scholar 

  • Hashem F M and Angle J S 1990 Rhizobiophage effects on nodulation, nitrogen fixation, and yield of field-grown soybeans (Glycine max L. Merr.). Biol. Fertil. Soils 9, 330–334.

    Google Scholar 

  • Hegde S V and Brahmaprakash G P 1992 A dry granular inoculant of Rhizobium for soil application. Plant and Soil 144, 309–311.

    Google Scholar 

  • Heichel G H 1982 Breeding alfalfa for improved nitrogen fixation: a physiological perspective. Iowa State J. Res. 56, 255–280.

    Google Scholar 

  • Hely F W 1964 Calcium carbonate as an aid to clover establishment in soils with low winter temperatures. Fld Stn Rec., Div. Plant Ind., CSIRO (Aust.) 3(1), 63–68.

    Google Scholar 

  • Hely F W, Hutchings R J and Zorin M 1980 Methods of rhizobial inoculation and sowing techniques for Trifolium subterranean L. establishment in a harsh winter environment. Aust. J. Agric. Res. 31, 703–712.

    Google Scholar 

  • Helyar K R 1976 Nitrogen cycling and soil acidification. J. Aust. Inst. Agric. Sci. 42, 217–221.

    Google Scholar 

  • Hennecke H 1990 Nitrogen fixation genes involved in the Bradyrhizobium japonicum-soybean symbiosis. FEBS Lett. 268, 422–426.

    Article  PubMed  Google Scholar 

  • Hennecke H, Alvarez-Morales A, Betancourt-Alvarez M, Ebeling S, Filser M, Fischer H-M, Gubler M, Hahn M, Kaluza K, Lamb J W, Meyer L, Regensburger B, Struder D and Weber J 1985 Organization and regulation of symbiotic nitrogen fixation genes from Bradyrhizobium japonicum. In Nitrogen Fixation Research Progress. Eds. H J Evans, P J Bottomley and W E Newton. pp 157–163. Martinus Nijhoff, Dordrecht.

    Google Scholar 

  • Herridge D F and Betts J H 1988 Field evaluation of soybean genotypes selected for enhanced capacity to nodulate and fix nitrogen in the presence of nitrate. Plant and Soil 110, 129–135.

    Google Scholar 

  • Herridge D F and Danso S K A 1995 Enhancing crop legume N2 fixation through selection and breeding. Plant and Soil 174.

  • Herridge D F and Roughley R J 1975 Variation in colony characteristics and symbiotic effectiveness of Rhizobium. J. Appl. Bacteriol. 38, 19–27.

    PubMed  Google Scholar 

  • Herridge D F, Roughley R J and Brockwell J 1984 Effect of rhizobia and soil nitrate on the establishment and functioning of the soybean symbiosis in the field. Aust. J. Agric. Res. 35, 149–161.

    Google Scholar 

  • Heyn C C 1963 The Annual Species of Medicago. Scripta Hierosolymitana, Vol. 12. pp 1–154. Hebrew Univ. Jerusalem.

    Google Scholar 

  • Hiltbold A E, Patterson R M and Reed R B 1985 Soil populations of Rhizobium japonicum in a cotton-corn-soybean rotation. Soil Sci. Soc. Am. J. 49, 343–348.

    Google Scholar 

  • Hiltbold A E, Thurlow D L and Skipper H D 1980 Evaluation of commercial soybean inoculants by various techniques. Agron. J. 72, 675–681.

    Google Scholar 

  • Hoben H J, Aung N N, Somasegaran P and Kang U G 1991 Oils as adhesives for seed inoculation and their influence on the survival of Rhizobium spp. and Bradyrhizobium spp. on inoculated seeds. World J. Microbiol. Biotechnol. 7, 324–330.

    Article  Google Scholar 

  • Hoben H J and Somasegaran P 1992 A small glass fermentor for the production of Rhizobium inoculum. World J. Microbiol. Biotechnol. 8, 333–334.

    Google Scholar 

  • Horvath B, Bachem C W B, Schell J and Kondorosi A 1987 Hostspecific regulation of nodulation genes in Rhizobium is mediated by a plant-signal, interacting with the nodD gene product. EMBO J. 6, 841–848.

    Google Scholar 

  • Howieson J G and Ewing M A 1986 Acid tolerance in the Rhizobium meliloti-Medicago symbiosis. Aust. J. Agric. Res. 37, 55–64.

    Google Scholar 

  • Howieson J G and Ewing M A 1986 Annual species of Medicago differ greatly in their ability to nodulate on acid soils. Aust. J. Agric. Res. 40, 843–850.

    Google Scholar 

  • Howieson J G, Ewing M A and d'Antuono M F 1988 Selection for acid tolerance in Rhizobium meliloti. Plant and Soil 105, 179–188.

    Google Scholar 

  • Hughes D Q and Vincent J M 1942 Serological studies of the root-nodule bacteria. III. Tests of neighbouring strains of the same species. Proc. Linn. Soc. NSW 67, 141–152.

    Google Scholar 

  • Hume D J and Blair D H 1992 Effect of numbers of Bradyrhizobium japonicum applied in commercial inoculant on soybean yield in Ontario. Can. J. Microbiol. 38, 588–593.

    Google Scholar 

  • Ireland J A and Vincent J M 1968 A quantitative study of competition for nodule formation. Trans 9th Int. Congr. Soil Sci., Vol. 2. pp 85–93. Augus and Robertson, Sydney, Australia.

    Google Scholar 

  • Itoh S and Barber S A 1983 Phosphorus uptake by six plant species as related to root hairs. Agron. J. 75, 457–461.

    Google Scholar 

  • Jansen van Rensberg H and Strijdom B W 1985 Survival of fast and slow-growing Rhizobium spp. under conditions of relatively mild desiccation. Soil Biol. Biochem. 12, 335–356.

    Google Scholar 

  • Jansen van Rensberg H and Strijdom B W 1985 Effectiveness of Rhizobium strains used in inoculants after their introduction into soil. Appl. Environ. Microbiol. 49, 127–131.

    Google Scholar 

  • Jarvis B D W, Downer H L and Young J P W 1992 Phylogeny of fast-growing soybean-nodulating rhizobia supports synonymy of Sinorhizobium and Rhizobium and assignment to Rhizobium fredii. Int. J. Syst. Bacteriol. 42, 93–96.

    PubMed  Google Scholar 

  • Jawson M D, Franzluebbers A J and Berg R K 1989 Bradyrhizobium japonicum survival in and soybean inoculation with fluid gels. Appl. Environ. Microbiol. 55, 617–622.

    Google Scholar 

  • Jenkins H V, Vincent J M and Waters L M 1954 The root-nodule bacteria as factors in clover establishment in the red basaltic soils of the Lismore district, New South Wales. III. Field inoculation trials. Aust. J. Agric. Res. 5, 77–89.

    Google Scholar 

  • Jenkins M B and Bottomley P J 1985 Seasonal response of uninoculated alfalfa to N fertilizer: soil N, nodule turnover, and symbiotic effectiveness of Rhizobium meliloti. Agron. J. 76, 959–963.

    Google Scholar 

  • Jenkins M B, Virginia R A and Jarrell W M 1987 Rhizobial ecology of the woody legume mesquite (Prosopis glandulosa) in the Sonoran desert. Appl. Environ. Microbiol. 53, 36–40.

    Google Scholar 

  • Jensen E S 1987 Inoculation of pea by application of Rhizobium in the planting furrow. Plant and Soil 97, 63–70.

    Google Scholar 

  • Johnson H W and Means U M 1963 Serological groups of Rhizobium japonicum recovered from nodules of soybeans (Glycine max) in field soils. Agron. J. 55, 269–271.

    Google Scholar 

  • Johnson H W, Means U M and Weber C R 1965 Competition for nodules sites between strains of Rhizobium japonicum applied as inoculum and strains in the soil. Agron. J. 57, 179–185.

    Google Scholar 

  • Johnston A W B and Beringer J E 1977 Chromosomal recombination between Rhizobium species. Nature, London 267, 611–613.

    Google Scholar 

  • Jones D G 1991 Symbiotic nitrogen fixation-exploitation and unachieved potential. Ann. Appl. Biol. 118, 249–259.

    Google Scholar 

  • Jones D G and Burrows A C 1969 Acid production and symbiotic effectiveness in Rhizobium trifolii. Soil Biol. Biochem. 1, 57–61.

    Article  Google Scholar 

  • Jones R and Giddens J 1984 Introduction of effective N2-fixing rhizobial strains into the soybean plant by use of fungicide resistance. Agron. J. 76, 599–602.

    Google Scholar 

  • Jordan D C 1982 Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria from leguminous plants. Int. J. Syst. Bacteriol. 32, 136–139.

    Google Scholar 

  • Jung G, Mugnier J, Diem H G and Dommergues Y R 1982 Polymerentrapped rhizobium as an inoculant for legumes. Plant and Soil 65, 219–231.

    Google Scholar 

  • Kamicker B J and Brill W J 1986 Identification of Bradyrhizobium japonicum nodule isolates from Wisconsin soybean farms. Appl. Environ. Microbiol. 51, 487–492.

    Google Scholar 

  • Kecskés M 1970 Comparative investigations of the action of fungicides on Rhizobium leguminosarum F. and its symbiosis with Vicia sativa. Meded. Fac. Landbouwwet. Rijksuniv. Gent 35, 505–514.

    Google Scholar 

  • Keyser H H and Li F 1992 Potential for increasing biological nitrogen fixation in soybean. Plant and Soil 141, 119–135.

    Google Scholar 

  • Keyser H H and Munns D N 1979 Tolerance of rhizobia to acidity, aluminium and phosphate. Soil Sci. Soc. Am. J. 43, 519–523.

    Google Scholar 

  • Keyser H H, Munns D N and Hohenberg J S 1979 Acid tolerance of rhizobia in culture and in symbiosis with cowpea. Soil Sci. Soc. Am. J. 43, 719–722.

    Google Scholar 

  • Keyser H H, Somasegaran P and Bohlool B B 1992 Rhizobial ecology and technology. In Soil Microbial Ecology. Applications in Agricultural and Environmental Management. Ed. F B MettingJr. pp 205–226. Marcel Dekker, New York.

    Google Scholar 

  • Kingsley M T and Bohlool B B 1981 Release of Rhizobium spp. from tropical soils and recovery for immunofluorescence enumeration. Appl. Environ. Microbiol. 42, 241–248.

    Google Scholar 

  • Kinkle B K, Sadowsky M J, Johnstone K and Koskinen W C 1994 Tellurium and selenium resistance in rhizobia and its potential use for direct isolation of Rhizobium meliloti from soil. Appl. Environ. Microbiol. 60, 1674–1677.

    Google Scholar 

  • Kinkle B K, Sadowsky M J, Schmidt E L and Koskinen W C 1993 Plasmids pJP4 and r68.45 can be transferred between populations of bradyrhizobia in nonsterile soil. Appl. Environ. Microbiol. 59, 1762–1766.

    Google Scholar 

  • Kinkle B K and Schmidt E L 1991 Transfer of the pea symbiotic plasmid pJB5JI in nonsterile soil. Appl. Environ. Microbiol. 57, 3264–3269.

    Google Scholar 

  • Kipe-Nolt J A, Montealegre M and Tohme J 1992 Restriction of nodulation by the broad host range Rhizobium tropici strain CIAT899 in wild accessions of Phaseolus vulgaris L. New Phytol. 120, 489–494.

    Google Scholar 

  • Kishinevsky B D, Lobel R, Gurfel D and Nemas C 1992 Soil fumigation with methyl bromide as a means of increasing the occurrence of the inoculum strain in peanut nodules. Soil Biol. Biochem. 24, 845–848.

    Article  Google Scholar 

  • Klucas R V, Hanus F J, Russell S A and Evans H J 1983 Nickel: A micronutrient element for hydrogen-dependent growth of Rhizobium japonicum and for expression of urease activity in soybean leaves. Proc. Natl. Acad. Sci. USA 80, 2253–2257.

    Google Scholar 

  • Kondorosi A, Horvath B, Gottfert M, Putnoky P, Rastas K, Gyorgypal Z, Kondorosi E, Torok I, Bachem C, John M, Schmidt J and Schell J 1985 Identification and organization of Rhizobium meliloti genes relevant to the initiation and development of nodules. In Nitrogen Fixation Research Progress. Eds. H J Evans, P J Bottomley and W E Newton. pp 73–78. Martinus Nijhoff, Dordrecht.

    Google Scholar 

  • Kosslak R M and Bohlool B B 1985 Influence of environmental factors on interstrain comptition in Rhizobium japonicum. Appl. Environ. Microbiol. 49, 1128–1153.

    Google Scholar 

  • Kosslak R M, Bookland R, Barkei J, Paaren H E and Appelbaum E R 1987 Induction of Bradyrhizobium japonicum common nod genes by isoflavones isolated from Glycine max. Proc. Natl. Acad. Sci. USA 84, 7428–7432.

    Google Scholar 

  • Kreig N R and Holt J G 1984 Bergey's Manual of Determinative Bacteriology, Vol. 1. Williams and Wilkins, Baltimore. 964p.

    Google Scholar 

  • Kremer R J and Peterson H L 1982 Effect of inoculant carrier on survival of Rhizobium on inoculated seed. Soil Sci. 134, 117–125.

    Google Scholar 

  • Kremer R J and Peterson H L 1983a Field evaluation of selected Rhizobium in an improved legume inoculant. Agron. J. 75, 139–143.

    Google Scholar 

  • Kremer R J and Peterson H L 1983b Effects of carrier and temperature on survival of Rhizobium spp. in legume inocula: development of an improved type of inoculant. Appl. Environ. Microbiol. 45, 1790–1794.

    Google Scholar 

  • Kremer R J, Polo J and Peterson H L 1982 Effect of suspending agent and temperature on survival of Rhizobium in fertilizer. Soil Sci. Soc. Am. J. 46, 539–542.

    Google Scholar 

  • Kucey R M N and Paul E A 1982 Carbon flow, photosynthesis and N2 fixation in mycorrhizal and nodulated faba beans (Vicia faba L.). Soil Biol. Biochem. 14, 407–412.

    Article  Google Scholar 

  • Kuykendall L D, Devine T E and Cregan P B 1982 Positive role of nodulation on the establishment of Rhizobium in subsequent crops of soybean. Can. J. Microbiol. 7, 79–81.

    Google Scholar 

  • Kuykendall L D and Elkan G H 1976 Rhizobium japonicum derivatives differing in nitrogen fixing efficiency and carbohydrate ulitization. Appl. Environ. Microbiol. 32, 511–519.

    PubMed  Google Scholar 

  • Kuykendall L D, Saxena B, Devine T E and Udell S E 1992 Genetic diversity in Bradyrhizobium japonicum Jordan 1982 and a proposal for Bradyrhizobium elkanii sp. nov. Can. J. Microbiol. 38, 501–503.

    Google Scholar 

  • Labandera C A and Vincent J M 1975 Loss of symbiotic capacity in commercially useful strains of Rhizobium trifolii. J. Appl. Bacteriol. 39, 209–211.

    PubMed  Google Scholar 

  • Ladha J K, Garcia M, Miyan S, Padre A T and Watanabe I 1989 Survival of Azorhizobium caulinodans in the soil and rhizosphere of wetland rice under Sesbania rostrata-rice rotation. Appl. Environ. Microbiol. 55, 454–460.

    Google Scholar 

  • Ladha J K, Pareek R P and Becker M 1992 Stem-nodulating legume-Rhizobium symbiosis and its agronomic use in lowland rice. In Advances in Soil Science, Vol. 20. pp 147–192. Springer-Verlag, New York.

    Google Scholar 

  • Ladha J K, Pareek R P, So R and Becker M 1990 Stem-nodule symbiosis and its unusual properties. In Nitrogen Fixation: Achievement and Objectives. Eds. P M Gresshoff, L E Roth, G Stacey and W E Newton. pp 633–640. Chapman and Hall, New York.

    Google Scholar 

  • Ladha J K, Watanabe I and Saono S 1988 Nitrogen fixation by leguminous green manure and practices for its enhancement in tropical lowland rice. In Sustainable Agriculture: Green Manure in Rice Farming. pp 165–183. IRRI, Los Banos, Philippines.

    Google Scholar 

  • La Favre A K, Sinclair M J, La Favre J S and Eaglesham A R J 1991 Bradyrhizobium japonicum native to tropical soils: novel sources of strains for inoculants for US-type soya bean. Trop. Agric. 68, 243–248.

    Google Scholar 

  • La Favre J S and Eaglesham A R J 1984 Increased nodulation of “non-nodulating” (rj1rj1) soybean by high dose inoculation. Plant and Soil 80, 297–300.

    Google Scholar 

  • Lange R T 1961 Nodule bacteria associated with the indigenous Leguminosae of south-west Australia. J. Gen. Microbiol. 61, 351–359.

    Google Scholar 

  • Lawrie A C 1983 Relationships among rhizobia from native Australian legumes. Appl. Environ. Microbiol. 45, 1822–1828.

    Google Scholar 

  • Leung K, Strain S R, de Bruijn F J and Bottomley P J 1994a Genotypic and phenotypic comparisons of chromosomal types within an indigenous soil population of Rhizobium leguminosarum bv. trifolii. Appl. Environ. Microbiol. 60, 416–426.

    Google Scholar 

  • Leung K, Yap K, Dashti N and Bottomley P J 1994b Serological and ecological characteristics of a nodule-dominent serotype from an indigenous soil population of Rhizobium leguminosarum bv. trifolii. Appl. Environ. Microbiol. 60, 408–415.

    Google Scholar 

  • Lie T A 1978 Symbiotic specialization in pea plants: the requirement of specific Rhizobium strains for peas from Afghanistan. Ann. Appl. Biol. 88, 462–465.

    Google Scholar 

  • Lindstrom K, Lipsanen P and Kaijalainen S 1990 Stability of markers used for identification of two Rhizobium galegae inoculant strains after five years in the field. Appl. Environ. Microbiol. 56, 444–450.

    Google Scholar 

  • Loneragan J F, Meyer D, Fawcett R G and Anderson A J 1955 Linepelleted clover seeds for nodulation on acid soils. J. Aust. Inst. Agric. Sci. 21, 264–265.

    Google Scholar 

  • Long S R 1989 Rhizobium-legume nodulation: life together in the underground. Cell 56, 203–214.

    Article  PubMed  Google Scholar 

  • Lowther W L and Littlejohn R P 1984 Effect of strain of rhizobia, inoculation level, and pelleting on the establishment of oversown Lotus pedunculatus “Grasslands Maku”. N. Z. J. Exp. Agric. 12, 287–294.

    Google Scholar 

  • Lowther W L and Loneragan J F 1968 Calcium and nodulation in subterranean clover (Trifolium subterraneum L.). Plant Physiol. 43, 1362–1366.

    Google Scholar 

  • Lussenhop J 1993 Effects of two collembola species on nodule occupancy by two Bradyrhizobium japonicum strains. Soil Biol. Biochem. 25, 775–780.

    Article  Google Scholar 

  • McLaughlin M J, Malik K A, Memon K S and Idris M 1990 The role of phosphorus in nitrogen fixation in upland crops. Proc. Workshop Phosphorus Requirements for Sustainable Agriculture in Asia and Oceania. pp 295–305. IRRI, Los Banos, Philippines.

    Google Scholar 

  • Mackay A D and Barber S A 1985 Effect of soil moisture and phosphate level on root hair growth of corn roots. Plant and Soil 86: 321–331.

    Google Scholar 

  • Mahdi A A and Atabani I M A 1992 Response of Bradyrhizobium-inoculated soyabean and lablab bean to inoculation with vesicular-arbuscular mycorrhizae. Exp. Agric. 28, 399–407.

    Google Scholar 

  • Mahler R L and Wollum A GII, 1980 Influence of water potential on the survival of rhizobia in a Goldsboro loamy sand. Soil Sci. Soc. Am. J. 44, 988–992.

    Google Scholar 

  • Mahler R L and Wollum A GII, 1981 The influence of soil water potential and soil texture on the survival of Rhizobium japonicum and Rhizobium leguminosarum isolates in the soil. Soil Sci. Soc. Am. J. 45, 761–766.

    Google Scholar 

  • Mahler R L and Wollum A GII, 1982 Seasonal fluctuation of Rhizobium japonicum under a variety of field conditions in North Carolina. Soil Sci. 134, 317–324.

    Google Scholar 

  • Marshall K C 1969 Studies by microelectrophoretic and microscopic techniques of the sorption of illite and montmorillonite to rhizobia. J. Gen. Microbiol. 56, 301–306.

    Google Scholar 

  • Martin R C, Voldeng H D and Smith D L 1991 Nitrogen transfer from nodulating soybean to maize or to nonnodulating soybean in intercrops: the 15N dilution method. Plant and Soil 132, 53–63.

    Google Scholar 

  • Mary P, Dupuy N, Dolhem-Biremon C, Defives C and Tailliez R 1994 Differences among Rhizobium melilotii and Bradyrhizobium japonicum strains in tolerance to desiccation and storage at different relative humidities. Soil Biol. Biochem. 26, 1125–1132.

    Article  Google Scholar 

  • Masterson C L and Sherwood M T 1974 Selection of Rhizobium trifolii strains by white and subterranean clovers. Ir. J. Agric. Res. 13, 91–99.

    Google Scholar 

  • Materon L A and Weaver R W 1984 Toxicity of arrowleaf clover seed to Rhizobium trifolii. Agron. J. 76, 471–473.

    Google Scholar 

  • Materson L A and Weaver R W 1985 Inoculant maturity influences survival of rhizobia on seed. Appl. Environ. Microbiol. 49, 465–467.

    Google Scholar 

  • Matthews S, Powell A A and Spaeth S C 1988 Seedling vigour and susceptibility to diseases and pests. In World Crops: Cool Season Food Legumes. Ed. R J Summerfield. pp 619–625. Kluwer, Dordrecht.

    Google Scholar 

  • May S N and Bohlool B B 1983 Competition among Rhizobium leguminosarum strains for nodulation of lentils (Lens esculenta). Appl. Environ. Microbiol. 45, 960–965.

    Google Scholar 

  • Moawad H and Bohlool B B 1984 Competition among Rhizobium spp. for nodulation of Leucaena leucocephala in two tropical soils. Appl. Environ. Microbiol. 48, 5–9.

    Google Scholar 

  • Moawad H A, Ellis W R and Schmidt E L 1984 Rhizosphere response as a factor in competition among three serogroups of indigenous Rhizobium japonicum for nodulation of field-grown soybeans. Appl. Environ. Microbiol. 47, 607–612.

    Google Scholar 

  • Moënne-Loccoz Y, Sen D, Krause E S and Weaver R W 1994 Plasmid profiles of rhizobia used in inoculants and isolated from clover fields. Agron. J. 86, 117–121.

    Google Scholar 

  • Moore W E C and Moore L V H 1992 Index of the Bacterial and Yeast Nomenclatural Changes. American Society for Microbiology, Washington DC.

    Google Scholar 

  • Muldoon J F, Hume D J and Beversdorf W D 1980 Effects of seed- and soil-applied Rhizobium japonicum inoculants on soybeans in Ontario. Can. J. Plant Sci. 60, 399–409.

    Google Scholar 

  • Munevar F and Wollum A GII 1981 Growth of Rhizobium japonicum strains at temperatures above 27°C. Appl. Environ. Microbiol. 42, 272–276.

    Google Scholar 

  • Munns D N 1968a Nodulation of Medicago sativa in solution culture. II. Compensating effects of nitrate and of prior nodulation. Plant and Soil 28, 246–257.

    Google Scholar 

  • Munns D N 1968b Nodulation of Medicago sativa in solution culture. III. Effects of nitrate on root hairs and infection. Plant and Soil 29, 33–47.

    Google Scholar 

  • Munns D N 1970 Nodulation of Medicago sativa in solution culture. V. Calcium and pH requirements during infection. Plant and Soil 32, 90–107.

    Google Scholar 

  • Munns D N 1977 Mineral nutrition and the legume symbiosis. In A Treatise on Dinitrogen Fixation. Section IV. Agronomy and Ecology. Eds. R W F Hardy and A H Gibson. pp 353–391. John Wiley and Sons, New York.

    Google Scholar 

  • Munns D N and Mosse B 1980 Mineral nutrition of legume crops. In Advances in Legume Science. Eds. R J Summerfield and A H Bunting. p pp 115–125. HMSO, London.

    Google Scholar 

  • Mytton L R, Brockwell J and Gibson A H 1984 The potential for breeding an improved lucerne-Rhizobium symbiosis. 1. Assessment of genetic variability. Euphytica 33, 401–410.

    Article  Google Scholar 

  • Nambiar P T C, Ravishanker H N and Dart P J 1983 Effect of Rhizobium numbers on nodulation and dinitrogen fixation in groundnut. Exp. Agric. 19, 243–250.

    Google Scholar 

  • Nathanson K, Lawn R J, de Jabrun P L M and Byth D E 1984 Growth, nodulation and nitrogen accumulation by soybean in saturated soil culture. Field Crops Res. 8, 73–92.

    Article  Google Scholar 

  • Nayudu M and Rolfe B G 1987 Analysis of R-primes demonstrates that genes for broad host range nodulation of Rhizobium strain NGR234 are dispersed on the Sym plasmid. Mol. Gen. Genet. 206, 326–337.

    Article  Google Scholar 

  • Nishijima F, Evans W R and Vesper S J 1988 Enhanced nodulation of soybean by Bradyrhizobium in the presence of Pseudomonas fluorescens. Plant and Soil 111, 149–150.

    Google Scholar 

  • Norris D O 1956 Legumes and the Rhizobium symbiosis. Emp. J. Exp. Agric. 27, 247–270.

    Google Scholar 

  • Norris D O 1959 Rhizobium affinities of African species of Trifolium. Emp. J. Exp. Agric. 27, 87–97.

    Google Scholar 

  • Nutman P S 1946 Variation within strains of clover nodule bacteria in the size of nodule produced and in the effectivity of the symbiosis. J. Bacteriol. 51, 411–432.

    Google Scholar 

  • Nutman P S 1959 Some observations on root-hair infection by nodule bacteria. J. Exp. Bot. 10, 250–263.

    Google Scholar 

  • Nutman P S 1965 The relationship between nodule bacteria and the legume host in the rhizosphere and in the process of infection. In Ecology of Soil-Borne Pathogens. Eds. K F Baker W C Snyder. pp 231–247. Univ. California Press, Berkeley.

    Google Scholar 

  • Nutman P S 1973 Effects of antibacterial substances on Rhizobium. In Rothamsted Experimental Station Report for 1972, part 1. pp 83–84. Rothamsted Experimental Station, Harpenden, UK.

    Google Scholar 

  • O'Hara G W, Boonkerd N and Dilworth M J 1988 Mineral constraints to nitrogen fixation. Plant and Soil 108, 93–110.

    Google Scholar 

  • Odeyemi O and Alexander M 1977 Use of fungicide-resistant rhizobia for legume inoculation. Soil Biol. Biochem. 9, 247–251.

    Article  Google Scholar 

  • Olsen P E, Rice W A and Collins M M 1995 Biological contaminants in North American rhizobial legume inoculants. Soil Biol. Biochem. 27 (In press).

  • Osa-Afiana L O and Alexander M 1979 Effect of moisture on the survival of Rhizobium in soil. Soil Sci. Soc. Am. J. 43, 925–930.

    Google Scholar 

  • Paau A S 1989 Improvement of Rhizobium inoculants. Appl. Environ. Microbiol. 55, 862–865.

    Google Scholar 

  • Paau A S 1991 Improvement of Rhizobium inoculants by mutation, genetic engineering and formulation. Biotechnol. Adv. 9, 173–184.

    Article  PubMed  Google Scholar 

  • Pareek R P, Ladha J K and Watanabe I 1990 Estimating N2 fixation by Sesbania rostrata and S. cannabina (syn. S. aculeata) in lowland rice by the 15N dilution method. Biol. Fertil. Soils 10, 77–88.

    Google Scholar 

  • Park S J and Buttery B R 1988 Nodulation mutants of white bean (Phaseolus vulgaris L.) induced by ethyl-methane sulphonate. Can. J. Plant Sci. 68, 199–202.

    Google Scholar 

  • Parke D and Ornston L N 1984 Nutritional diversity of Rhizobiaceae revealed by auxanography. J. Gen. Microbiol. 130, 1743–1750.

    Google Scholar 

  • Parke D and Ornston L N 1986 Enzymes of the β-ketoadipate pathway are inducible in Rhizobium and Agrobacterium spp. and constitutive in Bradyrhizobium spp. J. Bacteriol. 165, 288–292.

    PubMed  Google Scholar 

  • Parke D, Rivelli M and Ornston L N 1985 Chemotaxis to aromatic and hydroaromatic acids: comparison of Bradyrhizobium japonicum and Rhizobium trifolii. J. Bacteriol. 113, 417–422.

    Google Scholar 

  • Parker C A and Oakley A E 1965 Reduced nodulation of lupins and serradella due to lime pelleting. Aust. J. Exp. Agric. Anim. Husb. 5, 144–146.

    Google Scholar 

  • Parker C A, Trinick M J and Chatel D L 1977 Rhizobia as soil and rhizosphere inhabitants. In A Treatise on Dinitrogen Fixation. Section IV. Agronomy and Ecology. Eds. R W F Hardy and A H Gibson. pp 311–352. John Wiley and Sons, New York.

    Google Scholar 

  • Parker F E and Vincent J M 1981 Sterilization of peat by gamma irradiation. Plant and Soil 61, 285–293.

    Google Scholar 

  • Partridge C D P and Yates M G 1982 Effect of chelating agents on hydrogenase in Azotobacter chroococcum. Biochem. J. 204, 339–344.

    PubMed  Google Scholar 

  • Paul E A and Voroney R P 1984 Field interpretation of microbial biomass activity measurements. In Current Perspectives in Microbial Ecology. Eds. M J Klug and C A Reddy. pp 509–514. American Society for Microbiology, Washington DC.

    Google Scholar 

  • Peoples M B, Faizah A W, Rerkasem B and Herridge D F 1989 Methods for Evaluating Nitrogen Fixation by Nodulated Legumes in the Field. ACIAR, Canberra. 76 p.

    Google Scholar 

  • Peoples M B, Herridge D F and Ladha J K 1995a Biological nitrogen fixation: an efficient source of nitrogen for sustainable agricultural production ? Plant and Soil 174.

  • Peoples M B, Ladha J K and Herridge D F 1995b Enhancing legume N2 fixation through plant and soil management. Plant and Soil 174.

  • Peters N K, Frost J W and Long S R 1986 A plant flavone, luteolin, induces expression of Rhizobium meliloti nodulation genes. Science 233, 977–980.

    PubMed  Google Scholar 

  • Phillips D A, Dakora F D, Leon-Barrios M, Sande E and Joseph C M 1993a Signals released from alfalfa regulate microbial activities in the rhizosphere. In New Horizons in Nitrogen Fixation. Eds. J Palacios, J Mora and W E Newton. pp 197–202. Kluwer Acad. Publ., Dordrecht.

    Google Scholar 

  • Phillips D A, Joseph C M and Maxwell C A 1993b Non-flavonoid inducers of nod genes in Rhizobium meliloti: apparent nodD2 activities released naturally from alfalfa seeds add new dimensions to rhizosphere biology. In Advances in Molecular Genetics of Plant-Microbe Interactions, Vol. 2. Eds. E W Nester and D P S Verma. pp 169–173. Kluwer Acad. Publ. Dordrecht.

    Google Scholar 

  • Pineda J A, Kipe-Nolt J A and Rojas E 1994 Rhizobium inoculation increases of bean and maize yields in intercrops on farms in the Peruvian sierra. Exp. Agric. 30, 311–318.

    Google Scholar 

  • Postgate J R 1984 Concluding remarks. In Advances in Nitrogen Fixation Research. Eds. C Veeger and W E Newton. pp ix-x. Martinus Nijhoff/Dr. Junk Publ., The Hague.

    Google Scholar 

  • Quackenbush F W, Carter A S and Shenberger L C 1961 Inspection of legume inoculants and growth substances. Inspection Rep. No. 28. Purdue Univ. Agric. Exp. Stn, Lafayette, IN. 8p.

    Google Scholar 

  • Ravuri V and Hume D J 1992 Performance of a superior Bradyrhizobium japonicum and a selected Sinorhizobium fredii strain with soybean cultivars. Agron. J. 84, 1051–1056.

    Google Scholar 

  • Rawsthorne S and Summerfeld R J 1984 An assessment of different techniques for inoculating Phaseolus vulgaris with Rhizobium. Exp. Agric. 20, 119–127.

    Google Scholar 

  • Recourt K, Schripsema J, Kijne J W, van Brussel A A N and Lugtenberg B J J 1991 Inoculation of Vicia sativa subsp. nigra roots with Rhizobium leguminosarum biovar viciae results in release of nod gene activating flavanones and chalcones. Plant Mol. Biol. 16, 841–852.

    Article  PubMed  Google Scholar 

  • Redmond J W, Batley M, Djordjevic M A, Innes R W, Kuempel P L and Rolfe B G 1986 Flavones induce expression of nodulation genes in Rhizobium. Nature, London 323, 632–635.

    Google Scholar 

  • Rennie R J, Rennie D A, Siripaibool C, Chaiwanakupt P, Boonkerd N and Snitwongse P 1988 N2 fixation in Thai soybeans: Effects of tillage and inoculation on 15N-determined N2 fixation in recommended cultivars and advanced breeding lines. Plant and Soil 112, 183–193.

    Google Scholar 

  • Renwick A and Jones D G 1986 The manipulation of white clover “host preference” for strains of Rhizobium trifolii in an upland soil. Ann. Appl. Biol. 108, 291–302.

    Google Scholar 

  • Rerkasem B, Rerkasem K, Peoples M B, Herridge D F and Bergersen F J 1988 Measurement of N2 fixation in maize (Zea mays-L.)-ricebean (Vigna umbellata [Thunb.] Ohwi and Ohashi) intercrops. Plant and Soil 108, 125–135.

    Google Scholar 

  • Rice W A and Olsen P E 1992 Effects of inoculation method and size of Rhizobium meliloti population in the soil on nodulation of alfalfa. Can. J. Soil Sci. 72, 57–67.

    Google Scholar 

  • Rice W A, Olsen P E and Leggett M E 1995 Co-culture of Rhizobium and phosphate-solubilizing fungus in sterile peat. Soil Biol. Biochem. 27 (In press).

  • Rice W A, Penney D C and Nyborg M 1977 Effects of soil acidity on rhizobia numbers, nodulation and nitrogen fxation by alfalfa and red clover. Can. J. Soil Sci. 57, 197–203.

    Google Scholar 

  • Richardson A E and Simpson R J 1989 Acid-tolerance and symbiotic effectiveness of Rhizobium trifolii associated with a Trifolium subterraneum L.-based pasture growing in an acid soil. Soil Biol. Biochem. 21, 87–95.

    Article  Google Scholar 

  • Richardson A E, Viccars L A, Watson J M and Gibson A H 1995 Differentiation of Rhizobium strains using the polymerase chain reaction with random and directed primers. Soil Biol. Biochem. 27 (In press).

  • Rinaudo G, Dreyfus B and Dommergues Y 1988 Stem-nodulating legumes as green manure for rice in West Africa. In Green Manure in Rice Farming. pp 97–109. IRRI, Los Banos, Philippines.

    Google Scholar 

  • Robert F M and Schmidt E L 1985 Response of three indigenous serogroups of Rhizobium japonicum to the rhizosphere of preemergent seedlings of soybeans. Soil Biol. Biochem. 17, 579–580.

    Article  Google Scholar 

  • Robinson A C 1969 Host selection for effective Rhizobium trifolii by red clover and subterranean clover in the field. Aust. J. Agric. Res. 20, 1053–1060.

    Google Scholar 

  • Robinson P E 1961 Root-knot nematodes and legume nodules. Nature, London 189, 506–507.

    Google Scholar 

  • Robson A D and Bottomley P J 1991 Limitations in the use of legumes in agriculture and forestry. In Biology and Biochemistry of Nitrogen Fixation. Eds. M J Dilworth and A R Glenn. pp 320–349. Elsevier, Amsterdam.

    Google Scholar 

  • Robson A D, O'Hara G W and Abbott L K 1981 Involvement of phosphorus in nitrogen fixation by subterranean clover (Trifolium subterraneum L.). Aust. J. Plant Physiol. 8, 427–436.

    Google Scholar 

  • Rogers D D, Warren R DJr and Chamblee D S 1982 Remedial postemergence inoculation with Rhizobium. Agron. J. 74, 613–619.

    Google Scholar 

  • Rolfe B G, Innes R W, Schofield P R, Watson J M, Sargent C L, Kuempel P L, Plazinski J, Canter-Cremers H and Djordjevic M A 1985 Plant secreted factors induce the expression of R. trifolii nodulation and host-range genes. In Nitrogen Fixation Research Progress. Eds. H J Evans, P J Bottomley and W E Newton. pp 79–85. Martinus Nijhoff, Dordrecht.

    Google Scholar 

  • Roskoski J P, Pepper I and Pardo E 1986 Inoculation of leguminous trees with rhizobia and VA mycorrhizal fungi. For. Ecol. Manage. 16, 57–68.

    Google Scholar 

  • Roughley R J, Blowes W M and Herridge D F 1976 Nodulation of Trifolium subterraneum L. by introduced rhizobia in competition with naturalized strains. Soil Biol. Biochem. 8, 403–407.

    Article  Google Scholar 

  • Roughley R J and Brockwell J 1987 Grain legumes and soil microorganisms. In Grain Legumes 1987 Research and Production Seminar. AIAS Occ. Publ. No. 28. Eds. D Rde Kantzow and M G May. pp 66–69. Aust. Inst. Agric. Sci., Sydney.

    Google Scholar 

  • Roughley R J, Bullard G K and Gemell L G 1984 The Australian Inoculants Research and Control Service. N.S.W. Dept. Agric., Gosford, NSW. 21p.

    Google Scholar 

  • Roughley R J, Gault R R, Gemell L G, Andrews J A, Brockwell J, Dunn B W, Griffith G W, Hartley E J, Hebb D M, Peoples M B and Thompson J A 1995 Autecology of Bradyrhizobium japonicum in soybean-rice rotations. Plant and Soil (In press).

  • Roughley R J, Gemell L G, Thompson J A and Brockwell J 1993 The number of Bradyrhizobium sp. (Lupinus) applied to seed and its effect on rhizosphere colonization, nodulation and yield of lupin. Soil Biol. Biochem. 25, 1453–1458.

    Article  Google Scholar 

  • Roughley R J and Vincent J M 1967 Growth and survival of Rhizobium spp. in peat culture. J. Appl. Bacteriol. 30, 362–376.

    Google Scholar 

  • Rupela O P, Toomsan B, Mittal S, Dart P J and Thompson J A 1987 Chickpea Rhizobium populations: survey of influence of season, soil depth and cropping pattern. Soil Biol. Biochem. 19, 247–252.

    Article  Google Scholar 

  • Sadowsky M J and Bohlool B B 1985 Differential expression of the pea symbiotic plasmid pJB5JI in genetically dissimilar backgrounds. Symbiosis 1, 125–138.

    Google Scholar 

  • Salema M P, Parker C A, Kidby D K and Chatel D L 1982 Death of rhizobia on inoculated seed. Soil Biol. Biochem. 14, 13–14.

    Article  Google Scholar 

  • Sanginga N, Vanlauwe B and Danso S K A 1995 Management of biological N2 fixation in alley cropping systems: estimation and contribution to N balance. Plant and Soil 174.

  • Schall E D, Shenberger L C and Swope A 1975 Inspection of legume inoculants and pre-inoculated seeds. Inspection Rep. No. 106. Purdue Univ. Agric. Exp. Stn Lafayette, IN. 12p.

    Google Scholar 

  • Schiffman J and Alper Y 1968a Effects of Rhizobium-inoculum placement on peanut inoculation. Exp. Agric. 4, 203–208.

    Google Scholar 

  • Schiffman J and Alper Y 1968b Inoculation of peanuts by application of Rhizobium suspensions into the planting furrows. Exp. Agric. 4, 219–226.

    Google Scholar 

  • Schmidt E L and Robert F M 1985 Recent advances in the ecology of Rhizobium. In Nitrogen Fixation Research Progress. Eds. H J Evans, P J Bottomley and W E Newton. pp 379–385. Martinus Nijhoff, Dordrecht.

    Google Scholar 

  • Schmidt E L, Zidwick M J and Abebe H M 1986 Bradyrhizobium japonicum serocluster 123 and diversity among member isolates. Appl. Environ. Microbiol. 51, 1212–1215.

    Google Scholar 

  • Schofield P R, Ridge R W, Rolfe B G, Shine J and Watson J M 1984 Host-specific nodulation is encoded on a 14 kb fragment in Rhizobium trifolii. Plant Mol. Biol. 3, 3–11.

    Google Scholar 

  • Scudder W T 1975 Rhizobium inoculation of soybeans for subtropical and tropical soils. 1. Initial field trials. Soil Crop Sci. Soc. Fla Proc. 34, 79–82.

    Google Scholar 

  • Segovia L, Pinero D, Palacios R and Martinez-Romero E 1991 Genetic structure of a soil population of nonsymbiotic Rhizobium leguminosarum. Appl. Environ. Microbiol. 57, 426–433.

    PubMed  Google Scholar 

  • Silsbury J H 1984 Comparison of the growth rates of dinitrogen fixing subterranean clover swards with those assimilating nitrate ions. Plant and Soil 80, 201–213.

    Google Scholar 

  • Singleton P W, AbdelMagid H M and Tavares J W 1985 Effect of phosphorus on the effectiveness of strains of Rhizobium japonicum. Soil Sci. Soc. Am. J. 49, 613–616.

    Google Scholar 

  • Singleton P W, Bohlool B B and Nakao P L 1992 Legume response to rhizobial inoculation in the tropics: myths and realities. In Myths and Science of Soils in the Tropics. SSSA Special Publ. No. 29. pp 135–155. Soil Sci. Soc. Am., Madison, WI.

    Google Scholar 

  • Singleton P W and Stockinger K R 1983 Compensation against ineffective nodulation in soybean. Crop Sci. 23, 69–72.

    Google Scholar 

  • Singleton P W and Tavares J W 1986 Inoculation response of legumes in relation to the number and effectiveness of indigenous Rhizobium populations. Appl. Environ. Microbiol. 51, 1013–1018.

    Google Scholar 

  • Skipper H D, Palmer J H, Giddens J E and Woodruff J M 1980 Evaluation of commercial soybean inoculants from South Carolina and Georgia. Agron. J. 72, 673–674.

    Google Scholar 

  • Smith F W 1982 Mineral nutrition of legumes. In Nitrogen Fixation in Legumes. Ed. J M Vincent. pp 155–172. Academic Press, Sydney.

    Google Scholar 

  • Smith R S 1992 Legume inoculant formulation and application. Can. J. Microbiol. 38, 485–492.

    Google Scholar 

  • Smith R S, Ellis M A and Smith R E 1980 Effect of Rhizobium japonicum inoculant rates on soybean nodulation in a tropical soil. Agron. J. 72, 505–508.

    Google Scholar 

  • Smith S E and Bowen G D 1979 Soil temperature, mycorrhizal infection and nodulation of Medicago truncatula and Trifolium subterraneum. Soil Biol. Biochem. 11, 469–473.

    Article  Google Scholar 

  • Smith S E and Daft M J 1977 Interactions between growth, phosphate content and N2 fixation in mycorrhizal Medicago sativa. Aust. J. Plant Physiol. 4, 403–413.

    Google Scholar 

  • Smith S E and Daft M J 1978 The effect of mycorrhizas on the phosphate content, nitrogen fixation and growth of Medicago sativa. In Microbial Ecology. Eds. M W Loutit and J A R Miles. pp 314–319. Springer-Verlag, Heidelberg.

    Google Scholar 

  • So R B, Ladha J K and Young J P W 1994 Photosynthetic symbionts of Aeschynomene spp. form a cluster with bradyrhizobia on the basis of fatty acid and ribosomal rRNA analyses. Int. J. Syst. Bacteriol. 44, 392–403.

    PubMed  Google Scholar 

  • Somasegaran P 1985 Inoculant production with diluted liquid cultures of Rhizobium spp. and autoclaved peat: evaluation of diluents, Rhizobium spp., peats, sterility requirements, storage, and plant effectiveness. Appl. Environ. Microbiol. 50, 398–405.

    Google Scholar 

  • Somasegaran P 1991 Inoculant production with emphasis on choice of carriers, methods of production, and reliability testing/quality assurance guidelines. In Expert Consultation on Legume Inoculant Production and Quality Control. Ed. J A Thompson. pp 87–106. FAO, Rome.

    Google Scholar 

  • Somasegaran P and Bohlool B B 1990 Single-strain versus multistrain inoculation: effect of soil mineral N availability on rhizobial strain effectiveness and competition for nodulation on chick-pea, soybean and dry bean. Appl. Environ. Microbiol. 56, 3298–3303.

    Google Scholar 

  • Somasegaran P and Halliday J 1982 Dilution of liquid Rhizobium cultures to increase production capacity of inoculant plants. Appl. Environ. Microbiol. 44, 330–333.

    Google Scholar 

  • Somasegaran P and Hoben H J 1994 The Handbook for Rhizobia: Methods in Legume-Rhizobia Technology. Springer Verlag, New York. 450p.

    Google Scholar 

  • Somasegaran P, Hoben H J and Burton J C 1992 A medium-scale fermentor for mass culture of rhizobia. World J. Microbiol. Biotechnol. 8, 335–336.

    Google Scholar 

  • Somasegaran P, Hoben H J and Gurgun V 1988 Effect of inoculation rate, rhizobial strain competition and nitrogen fixation in chickpea. Agron. J. 80, 68–73.

    Google Scholar 

  • Somasegaran P, Hoben H J and Lewinson L 1990 Symbiotic interactions of Phaseolus acutifolius and P. acutifolius × P. vulgaris hybrid progenies in symbiosis with Bradyrhizobium spp. and Rhizobium leguminosarum bv. phaseoli. Can. J. Microbiol. 37, 497–503.

    Google Scholar 

  • Souza V, Eguiarte L, Avila G, Cappello R, Gallardo C, Montoya J and Pinero D 1994 Genetic structure of Rhizobium etli biovar phaseoli associated with wild and cultivated bean plants (Phaseolus vulgaris and Phaseolus coccineus) in Morelos, Mexico. Appl. Environ. Microbiol. 60, 1260–1268.

    Google Scholar 

  • Streeter J G, Salminen S O, Beuerlein J E and Schmidt W H 1994 Factors influencing the synthesis of polysaccharide by Bradyrhizobium japonicum bacteroids in field-grown soybean nodules. Appl. Environ. Microbiol. 60, 2939–2943.

    Google Scholar 

  • Streeter J G, Salminen S O and Carslon R W 1991 Synthesis of polysaccharide in soybean nodules by Bradyrhizobium japonicum strains which are highly competitive for nodule formation. Proc. 13th North American Symbiotic Nitrogen Fixation Conf. p 12.

  • Strijdom B W and Jansen van Rensburg H 1981 Effect of steam sterilization and gamma irradiation of peat on quality of Rhizobium inoculants. Appl. Environ. Microbiol. 41, 1344–1347.

    Google Scholar 

  • Thiagarajan T R, Ames R N and Ahmed M H 1992 Response of cowpea (Vigna unguiculata) to inoculation with co-selected vesicular-arbuscular mycorrhizal fungi and Rhizobium strains in field trials. Can. J. Microbiol. 38, 573–576.

    Google Scholar 

  • Thies J E, Bohlool B B and Singleton P W 1991a Subgroups of the cowpea miscellany: symbiotic specificity within Bradyrhizobium spp. for Vigna unguiculata, Phaseolus iunatus, Arachis hypogaea and Macroptilium atropurpureum. Appl. Environ. Microbiol. 57, 1540–1545.

    Google Scholar 

  • Thies J E, Bohlool B B and Singleton P W 1992 Environmental effects on competition for nodule occupancy between introduced and indigenous rhizobia and among introduced strains. Can. J. Microbiol. 38, 493–500.

    Google Scholar 

  • Thies J E, Cook S E and Corner R J 1994 Use of Bayesian influence in a Geographical Information System to determine regional legume inoculation requirements. In Proceedings of Resource Technology '94. New Opportunities. Best Practice. pp 475–488. Australian Department of Resources, Melbourne.

    Google Scholar 

  • Thies J E, Singleton P W and Bohlool B B 1991b Influence of the size of indigenous rhizobial populations on establishment and symbiotic performance of introduced rhizobia on field-grown legumes. Appl. Environ. Microbiol. 57, 19–28.

    Google Scholar 

  • Thies J E, Singleton P W and Bohlool B B 1991c Modeling symbiotic performance of introduced rhizobia in the field by use of indices of indigenous population size and nitrogen status of the soil. Appl. Environ. Microbiol. 57, 29–37.

    Google Scholar 

  • Thies J E, Woomer P L and Singleton P W 1995 Enrichment of Bradyrhizobium spp. populations in soil due to cropping of the homologous host legume. Soil Biol. Biochem. 27 (In press).

  • Thompson J A 1980 Production and quality control of legume inoculants. In Methods for Evaluating Biological Nitrogen Fixation. Ed. F J Bergersen. pp 489–533. John Wiley and Sons, Chichester, UK.

    Google Scholar 

  • Thompson J A 1983 Production and quality control of carrier-based legume inoculants. Inform. Bull. No. 17. ICRISAT, Patancheru, India.

    Google Scholar 

  • Thompson J A, Bhromsiri A, Shutsrirung A and Lillakan S 1991 Native root-nodule bacteria of traditional soybean-growing areas of northern Thailand. Plant and Soil 135, 53–65.

    Google Scholar 

  • Thompson J A, Brockwell J and Roughley R J 1975 Preinoculation of legume seed. J. Aust. Inst. Agric. Sci. 41, 253–254.

    Google Scholar 

  • Thompsom J A and Vincent J M 1967 Methods of detection and estimation of rhizobia in soil. Plant and Soil 26, 72–84.

    Google Scholar 

  • Tong Z and Sadowsky M J 1994 A selective medium for the isolation and quantification of Bradyrhizobium japonicum and Bradyrhizobium elkanii from soils and inoculants. Appl. Environ. Microbiol. 60, 581–586.

    Google Scholar 

  • Trinick M J 1980 Relationships amongst the fast growing rhizobia of Lablab purpureus, Leucaena leucocephala, Mimosa spp., Acacia farnesiana and Sesbania grandiflora and their affinities with other rhizobial groups. J. Appl. Bacteriol. 49, 39–53.

    Google Scholar 

  • Trinick M J 1982 Biology. In Nitrogen Fixation. Vol. 2: Rhizobium. Ed. W J Broughton. pp 76–146. Clarendon Press, Oxford.

    Google Scholar 

  • Trinick M J 1985 Rhizobium strain competition for host nodulation. In World Soybean Research Conference III: Proceedings. Ed. R Shibles. pp 911–917. Westview Press, Boulder, CO.

    Google Scholar 

  • Triplett E W, Albrecht K A and Oplinger E S 1993 Crop rotation effects on populations of Bradyrhizobium japonicum and Rhizobium meliloti. Soil Biol. Biochem. 25, 781–784.

    Article  Google Scholar 

  • Triplett E W and Sadowsky M J 1992 Genetics of competition for nodulation in legumes. Annu. Rev. Microbiol. 46, 399–428.

    Article  PubMed  Google Scholar 

  • Troedson R J, Lawn R J, Byth D E and Wilson G L 1989 Response of field-grown soybean to saturated soil culture. 1. Patterns of biomass and nitrogen accumulation. Field Crops Res. 21, 171–187.

    Article  Google Scholar 

  • Turk D and Keyser H H 1992 Rhizobia that nodulate tree legumes: specificity of the host for nodulation and effectiveness. Can. J. Microbiol. 38, 451–460.

    Google Scholar 

  • Turk D, Keyser H H and Singleton P W 1993 Response of tree legumes to rhizbial inoculation in relation to the population density of indigenous rhizobia. Soil Biol. Biochem. 25, 75–81.

    Article  Google Scholar 

  • Valdivia B, Dughri M H and Bottomley P J 1988 Antigenic and symbiotic characterization of indigenous Rhizobium leguminosarum bv. trifolii recovered from root nodules of Trifolium pratense L. sown into subterranean clover pasture soils. Soil Biol. Biochem. 20, 267–274.

    Article  Google Scholar 

  • van Brussel A A N, Recourt K, Pees E, Spaink H P, Tak T, Wijffelman C A, Kijne J W and Lugtenberg B J J 1990 A biovar-specific signal of Rhizobium leguminosarum bv. viciae induces increased nodulation gene-inducing activity in root exudate of Vicia sativa subsp. nigra. J. Bacteriol. 172, 5394–5401.

    PubMed  Google Scholar 

  • van Egeraat A W S M 1975 The possible role of homoserine in the development of Rhizobium leguminosarum in the rhizosphere of pea seedlings. Plant and Soil 42, 381–386.

    Google Scholar 

  • Verdcourt B 1970 Studies in the Leguminosae-Papilionoideae for the ‘Flora of Tropical East Africa’: IV. Kew Bull. 24, 507–569.

    Google Scholar 

  • Vincent J E and Smith M S 1982 Evaluation of inoculant viability on commercially inoculated legume seed. Agron. J. 74, 921–922.

    Google Scholar 

  • Vincent J M 1944 Variation in the nitrogen fixing property of Rhizobium trifolii. Nature, London 153, 496–497.

    Google Scholar 

  • Vincent J M 1954a The root-nodule bacteria as factors in clover establishment in the red basaltic soils of the Lismore district, New South Wales. Aust. J. Agric. Res. 5, 55–60.

    Google Scholar 

  • Vincent J M 1954b The root-nodule bacteria of pasture legumes. Proc. Linn. Soc. NSW 79, iv-xxxii.

    Google Scholar 

  • Vincent J M 1962 Australian studies of the root-nodule bacteria. A review. Proc. Linn. Soc. NSW 87, 8–38.

    Google Scholar 

  • Vincent J M 1965 Environmental factors in the fixation of nitrogen by the legume. In Soil Nitrogen. Eds. W V Bartholomew and F C Clark. pp 384–435. Am. Soc. Agron., Madison, WI.

    Google Scholar 

  • Vincent J M 1970 A Manual for the Practical Study of Root-Nodule Bacteria. IBP Handbook No. 15. Blackwell, Oxford. 164p.

    Google Scholar 

  • Vincent J M 1974 Root-nodule symbiosis with Rhizobium. In The Biology of Nitrogen Fixation. Ed. A Quispel. pp 265–341. North-Holland, Amsterdam.

    Google Scholar 

  • Wang C L, Beringer J E and Hirsch P R 1986 Host plant effects on hybrids of Rhizobium leguminosarum biovars viceae and trifolii. J. Gen. Microbiol. 132, 2063–2070.

    Google Scholar 

  • Weaver R W and Frederick L R 1974 Effect of inoculum rate on competitive nodulation of Glycine max L. Merrill. II. Field studies. Agron. J. 66, 233–236.

    Google Scholar 

  • Weaver R W, Morris D R, Boonkerd N and Sij J 1987 Populations of Bradyrhizobium japonicum in fields cropped with soybean-rice rotations. Soil Sci. Soc. Am. J. 51, 90–92.

    Google Scholar 

  • Wedderburn M E 1986 Effect of applied nitrogen, increased inoculation, broadcast lime, and seed pelleting on establishment of Lotus pedunculatus cv. “Grassland Maku” in tussock grasslands. N. Z. J. Exp. Agric. 14, 31–46.

    Google Scholar 

  • Weisz P R, Denison R F and Sinclair T R 1985 Response to drought stress of nitrogen fixation (acetylene reduction) rates by field-grown soybeans. Plant Physiol. 78, 525–530.

    Google Scholar 

  • Welsh J and McClelland M 1990 Fingerprinting genomes using PCR with arbitrary primers. Nucl. Acids Res. 18, 7213–7218.

    PubMed  Google Scholar 

  • Williams C H 1980 Soil acidifcation under clover pasture. Aust. J. Exp. Agric. Anim. Husb. 20, 561–567.

    Google Scholar 

  • Williams J G K, Kubelik A R, Livak K J, Rifalski J A and Tingey S V 1990 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18, 6531–6535.

    PubMed  Google Scholar 

  • Williams P M 1984 Current use of legume inoculant technology. In Biological Nitrogen Fixation, Ecology, Technology and Physiology. Ed. M Alexander. pp 173–200. Plenum Press, New York.

    Google Scholar 

  • Wilson J K 1944 Over five hundred reasons for abandoning the cross-inoculation groups of the legumes. Soil Sci. 58, 61–69.

    Google Scholar 

  • Wollum A GII and Cassel D K 1984 Spatial variability of Rhizobium japonicum in two North Carolina soils. Soil Sci. Soc. Am. J. 48, 1082–1086.

    Google Scholar 

  • Woomer P, Singleton P W and Bohlool B B 1988 Ecological indicators of native rhizobia in tropical soils. Appl. Environ. Microbiol. 54, 1112–1116.

    Google Scholar 

  • Woomer P L 1990 Predicting the abundance of indigenous and the persistence of introduced rhizobia in tropical soils. Ph.D. thesis, University of Hawaii.

  • Young J P W 1985 Rhizobium population genetics: enzyme polymorphism in isolates from peas, clover, bean and lucerne grown at the same site. J. Gen. Microbiol. 131, 2399–2408.

    Google Scholar 

  • Young J P W, Demetriou L and Apte R G 1987 Rhizobium population genetics: enzyme polymorphism in Rhizobium leguminosarum from plants and soil in a pea crop. Appl. Environ. Microbiol. 53, 397–402.

    Google Scholar 

  • Zaat S A J, Wijffelman C A, Spaink H P, van Brussel A A N, Okker R J H and Lugtenberg B J J 1987 Induction of the nodA promoter of Rhizobium leguminosarum sym plasmid pRL1JI by plant flavanones and flavones. J. Bacteriol. 169, 198–204.

    PubMed  Google Scholar 

  • Zahran H H and Sprent J I 1986 Effects of sodium chloride and polyethylene glycol on root-hair infection and nodulation of Vicia faba L. plants by Rhizobium leguminosarum. Planta 167, 303–309.

    Google Scholar 

  • Zhou J C, Tchan Y T and Vincent J M 1985 Reproduction capacity of bacteroids in nodules of Trifolium repens L. and Glycine max (L.) Merr. Planta 163, 473–482.

    Google Scholar 

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Brockwell, J., Bottomley, P.J. & Thies, J.E. Manipulation of rhizobia microflora for improving legume productivity and soil fertility: A critical assessment. Plant Soil 174, 143–180 (1995). https://doi.org/10.1007/BF00032245

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