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Somatic embryogenesis and plant regeneration from callus culture of Acacia catechu-a multipurpose leguminous tree

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Abstract

Plant regeneration via somatic embryogenesis was achieved from callus derived from immature cotyledons of Acacia catechu Willd. on Woody Plant Medium (WPM) supplemented with 13.9 μM kinetin and 2.7 μM 1-naphthaleneacetic acid. The addition of 0.9–3.5 mM L-proline to the medium influenced development of somatic embryos and also promoted secondary somatic embryogenesis. The light-green somatic embryos germinated on half-strength MS medium supplemented with 2% (w/v) sucrose. Somatic embryos germinated into plantlets that were acclimatized in the greenhouse and subsequently transferred to the field.

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Regional Plant Resource Centre, 751015, Bhubaneswar, Orissa, India

    G. R. Rout, S. Samantaray & P. Das

Authors
  1. G. R. Rout
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  2. S. Samantaray
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  3. P. Das
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Rout, G.R., Samantaray, S. & Das, P. Somatic embryogenesis and plant regeneration from callus culture of Acacia catechu-a multipurpose leguminous tree. Plant Cell Tiss Organ Cult 42, 283–285 (1995). https://doi.org/10.1007/BF00030000

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  • DOI: https://doi.org/10.1007/BF00030000

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