Abstract
Incubation of spinach thylakoids with HgCl2 selectively destroys Fe−S center B (FB). The function of electron acceptors in FB-less PS I particles was studied by following the decay kinetics of P700+ at room temperature after multiple flash excitation in the absence of a terminal electron acceptor. In untreated particles, the decay kinetics of the signal after the first and the second flashes were very similar (t 1/2∼2.5 ms), and were principally determined by the concentration of the artificial electron donor added. The decay after the third flash was fast (t 1/2∼0.25 ms). In FB-less particles, although the decay after the first flash was slow, fast decay was observed already after the second flash. We conclude that in FB-less particles, electron transfer can proceed normally at room temperature from FX to FA and that the charge recombination between P700+ and FX -/A1 - predominated after the second excitation. The rate of this recombination process is not significantly affected by the destruction of FB. Even in the presence of 60% glycerol, FB-less particles can transfer electrons to FA at room temperature as efficiently as untreated particles.
Similar content being viewed by others
Abbreviations
- DCIP:
-
2, 6-dichlorophenol indophenol
- FA, FB, FX :
-
iron-sulfur center A, B and X, respectively
- PMS:
-
phenazine methosulfate
References
Andréasson LE and Vänngård T (1988) Electron transport in Photosystems I and II. Ann Rev Plant Physiol 39: 379–411
Boardman NK (1971) Subchloroplast fragments: Digitonin method. Methods in Enzymol 23: 268–276
Bottin H and Mathis P (1985) Interaction of plastocyanin with the photosystem I reaction center: A kinetic study by flash absorption spectroscopy. Biochemistry 24: 6453–6460
Bottin H, Sétif P and Mathis P (1987) Study of the PS-I acceptor side by double and triple flash experiments. Biochim Biophys Acta 894: 39–48
Brettel K (1989) New assignment for the 250 μs kinetics in Photosystem I: P-700+ recombines with A1 - (not FX -). Biochim Biophys Acta 976: 246–249
Evans MCW and Heathcote P (1980) Effects of glycerol on the redox properties of the electron acceptor complex in spinach Photosystem I particles. Biochim Biophys Acta 590: 89–96
Fujii T, Yokoyama E, Inoue K and Sakurai H (1990) The sites of electron donation of Photosystem I to methyl viologen. Biochim Biophys Acta 1015: 41–48
Golbeck JH and Cornelius JM (1986) Photosystem I charge separation in the absence of centers A & B. I. Optical characterization of center ‘A2’ and evidence for its association with a 64-kDa peptide. Biochim Biophys Acta 849: 16–24
Golbeck JH, Velthuys BR and Kok B (1978) Evidence that the intermediate electron acceptor, A2, in Photosystem I is a bound iron-sulfur protein. Biochim Biophys Acta 504: 226–230
Golbeck JH and Warden JT (1982) Electron spin resonance studies of the bound iron-sulfur centers in photosystem I. Photoreduction of center A occurs in the absence of center B. Biochim Biophys Acta 681: 77–84
Hiyama T and Fork DC (1980) Kinetic identification of component X as P430: A primary electron acceptor of Photosystem I. Arch Biochem Biophys 199: 488–496
Hiyama T and Ke B (1971) A new photosynthetic pigment, ‘P430’: Its possible role as the primary electron acceptor of Photosystem I. Proc Natl Acad Sci USA 68: 1010–1013
Inoue K, Sakurai H and Hiyama T (1986) Photoinactivation sites of Photosystem I in isolated chloroplasts. Plant Cell Physiol 27: 961–968
Itoh S, Iwaki M and Ikegami I (1987) Extraction of vitamin K-1 from Photosystem I particles by treatment with diethyl ether and its effects on the A1 - EPR signal and system I photochemistry. Biochim Biophys Acta 893: 508–516
Koike H and Katoh S (1982) Spectral features of the bound electron acceptor A2 of photosystem I. Photochem Photobiol 35: 527–531
Kojima Y, Hiyama T and Sakurai H (1987a) Effects of mercurials on iron-sulfur centers of photosystem I of Anacystis nidulans In: Biggins J (ed) Progress in Photosynthesis Research, Vol 2, pp 57–60. Dordrecht: Martinus Nijhoff
Kojima Y, Niinomi Y, Tsuboi S, Hiyama T and Sakurai H (1987b) Destruction of Photosystem I iron-sulfur centers of spinach and Anacystis nidulans by mercurials. Bot Mag Tokyo 100: 243–253
Lagoutte B and Mathis P (1989) The Photosystem I reaction center: Structure and photochemistry. Photochem Photobiol 49: 833–844
Malkin R (1984) Diazonium modification of Photosystem I. A specific effect on iron-sulfur center B. Biochim Biophys Acta 764: 63–69
Malkin R (1987) Photosystem I. In: Barber J (ed) Topics in Photosynthesis Vol 8, pp 495–525. Amsterdam: Elsevier
Sakurai H and San Pietro A (1985) Association of Fe−S center(s) with the large subunit(s) of photosystem I particles. J Biochem 98: 69–76
Sauer K, Mathis P, Acker S and Van Best JA (1978) Electron acceptors associated with P-700 in Triton solubilized photosystem I particles from spinach chloroplasts. Biochim Biophys Acta 503: 120–134
Setif P, Ikegami I and Biggins J (1987) Light-induced charge separation in Photosystem I at low temperature is not influenced by vitamin K-1. Biochim Biophys Acta 894: 146–156
Shuvalov VA, Dolan E and Ke B (1979) Spectral and kinetic evidence for two early electron acceptors in photosystem I. Proc Natl Acad Sci USA 76: 770–773
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sakurai, H., Inoue, K., Fujii, T. et al. Effects of selective destruction of iron-sulfur center B on electron transfer and charge recombination in Photosystem I. Photosynth Res 27, 65–71 (1991). https://doi.org/10.1007/BF00029977
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00029977