Abstract
Recent advances in the study of GA mutants are reviewed. Endogenous GAs in the vast majority of GA synthesis and response mutants have now been quantified by physicochemical means, and the implications of the results are discussed. In recent papers the effects of synthesis mutations on processes other than stem elongation have received increased attention, as has the advent of mutants with reportedly elevated GA levels. The ‘feedback theory’ has been formulated, explaining paradoxical observations on endogenous GA levels in certain response mutants. In a significant breakthrough, a GA biosynthesis gene has been cloned, paving the way for a combined approach to future GA research, involving GA mutants, physicochemical analyses, and molecular techniques.
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Appleford NEJ and Lenton JR (1991) Gibberellins and leaf expansion in near-isogenic wheat lines containing Rht1 and Rht3 dwarfing alleles. Planta 183: 229–236
Beall FD, Morgan PW, Mander LN, Miller FR and Babb KH (1991) Genetic regulation of development in Sorghum bicolor. V. The ma R3 allele results in gibberellin enrichment. Plant Physiol 95: 116–125
Bensen RJ and Zeevaart JAD (1990) Comparison of ent-kaurene synthetase A and B activities in cell-free extracts from young tomato fruit of wild-type and gib-1, gib-2 and gib-3 tomato plants. J Plant Growth Regul 9: 237–242
Bohner J, Hedden P, Bora-Haber E and Bangerth F (1988) Identification and quantitation of gibberellins in fruits of Lycopersicon esculentum, and their relationship to fruit size in L. esculentum and L. pimpinellifolium. Physiol Plant 73: 348–353
Boother GM, Gale MD, Gaskin P, MacMillan J and Sponsel VM (1991) Gibberellins in shoots of Hordeum vulgare. A comparison between cv. Triumph and two dwarf mutants which differ in their response to gibberellin. Physiol Plant 81: 385–392
Campell BR and Bonner BA (1986) Evidence for phytochrome regulation of gibberellin A20 3β-hydroxylation in shoots of dwarf (lele) Pisum sativum L. Plant Physiol 82: 909–915
Chandler PM (1988) Hormonal regulation of gene expression in the “slender” mutant of barley (Hordeum vulgare L.) Planta 175: 115–120
Childs KL, Pratt LH and Morgan PW (1991) Genetic regulation of development in Sorghum bicolor. VI. The ma R3 allele results in abnormal phytochrome physiology. Plant Physiol 97: 714–719
Cramp RE and Reid JB (1993) Internode length in Pisum. Gene lkd. Plant Growth Regul 12: 141–147
Croker SJ, Hedden P, Lenton JR and Stoddart JL (1990) Comparison of gibberellins in normal and slender barley seedlings. Plant Physiol 94: 194–200
Devlin PF, Rood SB, Somers DE, Quail PH and Whitelam GC (1992) Photophysiology of the elongated internode (ein) mutant of Brassica rapa. Plant Physiol 100: 1442–1447
Digby J and Firn RD (1985) Growth substances and leaf growth. In: Baker NR, Davies WJ and Ong CK (eds) Control of Leaf Growth, SEB Seminar Series, Vol 27, pp 57–76. Cambridge: Cambridge University Press
Firn RD (1986) Growth substance sensitivity: The need for clearer ideas, precise terms and purposeful experiments. Physiol Plant 67: 267–272
Fujioka S, Yamane H, Spray CR, Gaskin P, MacMillan J, Phinney BO and Takahashi N (1988) Qualitative and quantitative analyses of gibberellins in vegetative shoots of normal, dwarf-1, dwarf-2, dwarf-3, and dwarf-5 seedlings of Zea mays L. Plant Physiol 88: 1367–1372
Fujioka S, Yamane H, Spray CR, Katsumi M, Phinney BO, Gaskin P, MacMillan J and Takahashi N (1988) The dominant non-gibberellin-responding dwarf mutant (D8) of maize accumulates native gibberellins. Proc Natl Acad Sci USA 85: 9031–9035
Fujioka S, Yamane H, Spray CR, Phinney BO, Gaskin P, MacMillan J and Takahashi N (1990) Gibberellin A3 is biosynthesized from gibberellin A20 via gibberellin A5 in shoots of Zea mays L. Plant Physiol 94: 127–131
Garcia-Martinez JL, Santes C, Croker SJ and Hedden P (1991) Identification, quantitation and distribution of gibberellins in fruits of Pisum sativum L. cv. Alaska during pod development. Planta 184: 53–60
Garcia-Martinez JL, Sponsel VM and Gaskin P (1987) Gibberellins in developing fruits of Pisum sativum cv. Alaska: Studies on their role in pod growth and seed development. Planta 170: 130–137
Graebe JE (1987) Gibberellin biosynthesis and control. Ann Rev Plant Physiol 38: 419–465
Gorter CJ (1961) Dwarfism of peas and the action of gibberellic acid. Physiol Plant 14: 332–343
Groot SPC, Bruinsma J and Karssen CM (1987) The role of endogenous gibberellin in seed and fruit development of tomato: Studies with a gibberellin-deficient mutant. Physiol Plant 71: 184–190
Harberd NP and Freeling M (1989) Genetics of dominant gibberellin-insensitive dwarfism in maize. Genetics 121: 827–838
Hedden P and Croker SJ (1992) Regulation of gibberellin biosynthesis in maize seedlings. In: Karssen CM, Van Loon LC and Vreugdenhil D (eds) Progress in Plant Growth Regulation, pp 534–544. Dordrecht: Kluwer
Hedden P and Lenton J (1988) Genetical and chemical approaches to the metabolic regulation and mode of action of gibberellins in plants. In: Steffens GL and Rumsey TS (eds) Biomechanisms Regulating Growth and Development. Beltsville Symposia in Agricultural Research Vol 12, pp 175–204. Boston: Kluwer
Hedden P and Phinney BO (1979) Comparison of ent-kaurene and ent-isokaurene synthesis in cell-free systems from etiolated shoots of normal and dwarf-5 seedlings. Phytochemistry 18: 1475–1479
Ingram TJ, Reid JB, Murfet IC, Gaskin P, Willis CL and MacMillan J (1984) Internode length in Pisum. The Le gene controls the 3β-hydroxylation of gibberellin A20 to gibberellin A1. Planta 160: 455–463
Jolly CJ, Reid JB and Ross JJ (1987) Internode length in Pisum. Action of gene lw. Physiol Plant 69: 489–498
Jones MG (1987) Gibberellins and the procera mutant of tomato. Planta 172: 280–284
Klee H and Estelle M (1991) Molecular genetic approaches to plant hormone biology. Ann Rev Plant Physiol Plant Mol Biol 42: 529–551
Kobayashi M, Sakurai A, Saka H and Takahashi N (1989) Quantitative analysis of endogenous gibberellins in normal and dwarf cultivars of rice. Plant Cell Physiol 30: 963–969
Koornneef M, Bosma TDG, Hanhart CJ, van der Veen JH and Zeevaart JAD (1990) The isolation and characterization of gibberellin-deficient mutants in tomato. Theor Appl Genet 80: 852–857
Lanahan MB and Ho THD (1988) Slender barley: A constitutive gibberellin-response mutant. Planta 175: 107–114
Lange T and Graebe JE (1989) The partial purification and characterization of a gibberellin C-20 hydroxylase from immature Pisum sativum L. seeds. Planta 179: 211–221
Lawrence NL, Ross JJ, Mander LN and Reid JB (1992) Internode length in Pisum. Mutants lk, lka, and lkb do not accumulate gibberellins. J Plant Growth Regul 11: 35–37
MacMillan J (1990) Metabolism of gibberellins A20 and A9 in plants: Pathways and enzymology. In: Pharis RP and Rood SB (eds) Plant Growth Substances 1988, pp 307–313. Berlin: Springer-Verlag
Metzger JD and Hassebrock AT (1990) Selection and characterization of a gibberellin-deficient mutant of Thlaspi arvense L. Plant Physiol 94: 1655–1662
Murakami Y (1972) Dwarfing genes in rice and their relation to gibberellin biosynthesis. In: Carr DJ (ed) Plant Growth Substances 1970, pp 166–174. Berlin: Springer-Verlag
Murofushi N, Honda I, Hirasawa R, Yamaguchi I, Takahashi N and Phinney BO (1990) Gibberellins from the tassel, cob, “seed”, silk, and pollen of maize. In: Takahashi N, Phinney BO and MacMillan J (eds) Gibberellins, pp 32–39. Ann Arbor: Springer-Verlag
Nadhzimov UK, Jupe SC, Jones MG and Scott IM (1988) Growth and gibberellin relations of the extreme dwarf d x tomato mutant. Physiol Plant 73: 252–256
Nagatani A, Reid JB, Ross JJ, Dunnewijk A and Furuya M (1990) Internode length in Pisum. The response to light quality, and phytochrome type I and II levels in lv plants. J Plant Physiol 135: 667–674
Nakayama I, Kobayashi M, Kamiya Y, Abe H and Sakurai A (1992) Effects of a plant-growth regulator, prohexadione-calcium (BX112), on the endogenous levels of gibberellins in rice. Plant Cell Physiol 33: 59–62
Ozga JA, Brenner ML and Reinecke DM (1992) Seed effects on gibberellin metabolism in pea pericarp. Plant Physiol 100: 88–94
Peng J and Harberd NP (1993) Derivative alleles of the Arabidopsis gibberellin-insensitive (gai) mutation confer a wild-type phenotype. Plant Cell 5: 351–360
Phinney BO, Freeling M, Robertson DS, Spray CR and Silverthorne J (1986) Dwarf mutants in maize-the gibberellin biosynthetic pathway and its molecular future. In: Bopp M (ed) Plant Growth Substances 1985, pp 55–64. Berlin: Springer-Verlag
Phinney BO and Spray CR (1990) Dwarf mutants of maize-research tools for the analysis of growth. In Pharis RP and Rood SB (eds) Plant Growth Substances 1988, pp 65–73. Berlin: Springer-Verlag
Potts WC and Reid JB (1983) Internode length in Pisum. III. The effect and interaction of the Na/na and Le/le gene differences on endogenous gibberellin-like substances. Physiol Plant 57: 448–454
Potts WC, Reid JB and Murfet IC (1985) Internode length in Pisum. Gibberellins and the slender phenotype. Physiol Plant 63: 357–364
Proebsting WM, Hedden P, Lewis MJ, Croker SJ and Proebsting LN (1992) Gibberellin concentration and transport in genetic lines of pea. Plant Physiol 100: 1354–1360
Rasmusson J (1927) Genetically changed linkage values in Pisum. Hereditas 10: 1–150
Reid JB (1983) Internode length in Pisum. Do the internode length genes effect growth in dark-grown plants? Plant Physiol 72: 759–763
Reid JB (1987) The genetic control of growth via hormones. In: Davies PJ (ed) Plant Hormones and Their Role in Plant Growth and Development, pp 318–340. Dordrecht: Martinus Nijhoff Publishers
Reid JB (1990) Phytohormone mutants in plant research. J Plant Growth Regul 9: 97–111
Reid JB (1993) Plant hormone mutants. J Plant Growth Regul 12: 207–226
Reid JB, Hasan O and Ross JJ (1990) Internode length in Pisum. Gibberellins and the response to far-red-rich light. J Plant Physiol 137: 46–52
Reid JB, Murfet IC and Potts WC (1983) Internode length in Pisum. II Additional information on the relationship and action of loci Le, La, Cry, Na and Lm. J Exp Bot 34: 349–364
Reid JB and Ross JJ (1988) Internode length in Pisum. A new gene, lv, conferring an enhanced response to gibberellin A1. Physiol Plant 72: 595–604
Reid JB and Ross JJ (1989) Internode length in Pisum. Two further gibberellin-insensitivity genes, lka and lkb. Physiol Plant 75: 81–88
Reid JB and Ross JJ (1993) A mutant-based approach, using Pisum sativum, to understanding plant growth. Int J Plant Sci 154: 22–34
Reid JB, Ross JJ and Hasan O (1991) Internode length in Pisum. Gene lkc. J Plant Growth Regul 10: 11–16
Reid JB, Ross JJ and Swain SM (1992) Internode length in Pisum. A new, slender mutant with elevated levels of C19 gibberellins. Planta 188: 462–467
Rood SB, Pearce D, Williams PH and Pharis RP (1989) A gibberellin-deficient Brassica mutant-rosette. Plant Physiol 89: 482–487
Rood SB, Williams PH, Pearce D, Murofushi N, Mander LN and Pharis RP (1990) A mutant gene that increases gibberellin production in Brassica. Plant Physiol 93: 1168–1174
Rood SB, Zanewich KP and Bray DF (1990) Growth and development of Brassica genotypes differing in endogenous gibberellin content. II. Gibberellin content, growth analyses and cell size. Physiol Plant 79: 679–685
Rood SB, Zanewich KP and Pearce DW (1993) Photocontrol of gibberellin metabolism in Brassica. Suppl Plant Physiol 102 Abs 37
Ross JJ, Davies NW, Reid JB and Murfet IC (1990) Internode length in Lathyrus odoratus. Effects of mutants l and lb on gibberellin metabolism and levels. Physiol Plant 79: 453–458
Ross JJ, Murfet IC and Reid JB (1990) Internode length in Lathyrus odoratus L.: The expression and interaction of genes L and Lb. J Hered 81: 201–204
Ross JJ, Murfet IC and Reid JB (1993) Distribution of gibberellins in Lathyrus odoratus L. and their role in leaf growth. Plant Physiol 102: 603–608
Ross JJ and Reid JB (1987) Internode length in Pisum. A new allele at the le locus. Ann Bot 59: 107–109
Ross JJ and Reid JB (1989) Internode length in Pisum. Biochemical expression of the le gene in darkness. Physiol Plant 76: 164–172
Ross JJ and Reid JB (1991) Internode length in Pisum:le 5839 is a less severe allele than Mendel's le. Pisum Genet 23: 29–32
Ross JJ, Reid JB, Davies NW and Murfet IC (1990) Internode length in Lathyrus odoratus. The involvement of gibberellins. Physiol Plant 79: 448–452
Ross JJ, Reid JB, Gaskin P and MacMillan J (1989) Internode length in Pisum. Estimation of GA1 levels in genotypes Le, le and le d. Physiol Plant 76: 173–176
Ross JJ, Reid JB and Dungey HS (1992) Ontogenetic variation in levels of gibberellin A1 in Pisum. Implications for the control of stem elongation. Planta 186: 166–171
Ross JJ, Reid JB and Swain SM (1993) Control of stem elongation by gibberellin A1: Evidence from genetic studies including the slender mutant sln. Aust J Plant Physiol 20: 585–599
Ross JJ, Willis CL, Gaskin P and Reid JB (1992) Shoot elongation in Lathyrus odoratus L.: Gibberellin levels in light- and dark-grown tall and dwarf seedlings. Planta 187: 10–13
Santes C, Hedden P, Sponsel VM, Reid JB and Garcia-Martinez JL (1993) Expression of the le mutation in young ovaries of Pisum sativum and its effect on fruit development. Plant Physiol 101: 759–764
Scott IM (1990) Plant hormone response mutants. Physiol Plant 78: 147–152
Sherriff LJ, McKay M, Ross JJ, Reid JB and Willis CL (1994) Decapitation reduces the metabolism of gibberellin A20 to A1 in Pisum sativum L., decreasing the Le/le difference. Plant Physiol 104: 277–280
Shi L, Gast RT, Gopalraj M and Olszewski NE (1992) Characterisation of a shoot-specific, GA3- and ABA-regulated gene from tomato. Plant J 2: 153–159
Smith VA (1992) Gibberellin A1 biosynthesis in Pisum sativum L. II. Biological and biochemical consequences of the le mutation. Plant Physiol 99: 372–377
Smith VA, Knatt CJ, Gaskin P and Reid JB (1992) The distribution of gibberellins in vegetative tissues of Pisum sativum L. I. Biological and biochemical consequences of the le mutation. Plant Physiol 99: 368–371
Spray C, Phinney BO, Gaskin P, Gilmour SJ and MacMillan J (1984) Internode length in Zea mays L. The dwarf-1 mutation controls the 3β-hydroxylation of gibberellin A20 to gibberellin A1. Planta 160: 464–468
Sponsel VM (1983) The localization, metabolism and biological activity of gibberellins in maturing and germinating seeds of Pisum sativum cv. Progress No. 9. Planta 159: 454–468
Sponsel VM (1986) Gibberellins in dark- and red-light-grown shoots of dwarf and tall cultivars of Pisum sativum: The quantification, metabolism and biological activity of gibberellins in Progress No. 9 and Alaska. Planta 168: 119–129
Steane DA, Ross JJ and Reid JB (1989) Metabolism of [3H]GA1 in a range of internode length mutants of Pisum. J Plant Physiol 135: 70–74
Stoddart JL (1984) Growth and gibberellin-A1 metabolism in normal and gibberellin-insensitive (Rht3) wheat (Triticum aestivum L.) seedlings. Planta 161: 432–438
Sun T, Goodman HM and Ausubel FM (1992) Cloning the Arabidopsis GA1 locus by genomic subtraction. Plant Cell 4: 119–128
Suttle JC and Zeevaart JAD (1979) Stem growth, flower formation, and endogenous gibberellins in a normal and a dwarf strain of Silene armeria. Planta 145: 175–180
Swain SM and Reid JB (1992) Internode length in Pisum. A new allele at the Lh locus. Physiol Plant 86: 124–130
Swain SM, Reid JB and Ross JJ (1993) Seed development in Pisum. The lh i allele reduces gibberellin levels in developing seeds, and increases seed abortion. Planta 191: 482–488
Talon M, Koornneef M and Zeevaart JAD (1990) Endogenous gibberellins in Arabidopsis thaliana and possible steps blocked in the biosynthetic pathways of the semidwarf ga4 and ga5 mutants. Proc Natl Acad Sci USA 87: 7983–7987
Talon M, Koornneef M and Zeevaart J (1990) Accumulation of C19-gibberellins in the gibberellin-insensitive dwarf mutant gai of Arabidopsis thaliana (L.) Heynh. Planta 182: 501–505
Talon M and Zeevaart JAD (1990) Gibberellins and stem growth as related to photoperiod in Silene armeria L. Plant Physiol 92: 1094–1100
Trewavas A (1981) How do plant growth substances work? Plant Cell Environ 4: 203–228
Trewavas A (1987) Sensitivity and sensory adaptation in growth substance responses. In: Hoad GV, Lenton JR, Jackson MB and Atkin RK (eds) Hormone Action in Plant Development — A Critical Appraisal, pp 19–38. London: Butterworths
Waycott W and Taiz L (1991) Phenotypic characterization of lettuce dwarf mutants and their response to applied gibberellins. Plant Physiol 95: 1162–1168
Waycott W, Smith VA, Gaskin P, MacMillan J and Taiz L (1991) The endogenous gibberellins of dwarf mutants of lettuce. Plant Physiol 95: 1169–1173
Weller JL, Ross JJ and Reid JB (1994) Gibberellins and phytochrome regulation of stem elongation in pea. Planta 192: 489–496
Zanewich KP, Rood SB, Southworth CE and Williams PH (1991) Dwarf mutants of Brassica: Responses to applied gibberellins and gibberellin content. J Plant Growth Regul 10: 121–127
Zanewich KP, Rood SB and Williams PH (1990) Growth and development of Brassica genotypes differing in endogenous gibberellin content. I Leaf and reproductive development. Physiol Plant 79: 673–678
Zeevaart JAD and Talon M (1992) Gibberellin mutants in Arabidopsis thaliana. In: Karssen CM, Van Loon LC and Vreugdenhil D (eds) Progress in Plant Growth Regulation, pp 34–42. Dordrecht: Kluwer
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Ross, J.J. Recent advances in the study of gibberellin mutants. Plant Growth Regul 15, 193–206 (1994). https://doi.org/10.1007/BF00029892
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DOI: https://doi.org/10.1007/BF00029892