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Isolation of cytochrome bc 1 complexes from the photosynthetic bacteria Rhodopseudomonas viridis and Rhodospirillum rubrum

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Abstract

Cytochrome bc 1 complexes have been isolated from wild type Rhodopseudomonas viridis and Rhodospirillum rubrum and purified by affinity chromatography on cytochrome c-Sepharose 4B. Both complexes are largely free of bacteriochlorophyll and carotenoids and contain cytochromes b and c 1 in a 2:1 molar ratio. For the Rps. viridis complex, evidence has been obtained for two spectrally distinct b-cytochromes. The R. rubrum complex contains a Rieske iron-sulfur protein (present in approximately 1:1 molar ratio to cytochrome c 1) and catalyzes an antimycin A- and myxothiazol-sensitive electron transfer from duroquinol to equine cytochrome c or R. rubrum cytochrome c 2. Although an attempt to prepare a cytochrome bc 1 complex from the gliding green bacterium Chloroflexus aurantiacus was not successful, membranes isolated from phototrophically grown Cfl. aurantiacus were shown to contain a Rieske iron-sulfur protein and protoheme (the prosthetic group of b-type cytochromes).

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Author notes

  1. Dale F. Gaul

    Present address: Department of Biochemistry, University of Wisconsin, 53706, Madison, Wisconsin, USA

  2. Won-Ki Choi

    Present address: Department of Chemistry, Chonnam National University, 500, Kwangju, Chonnam, Republic of Korea

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Texas Tech University, 79409, Lubbock, Texas, USA

    R. Max Wynn, Dale F. Gaul, Won-Ki Choi, Robert W. Shaw & David B. Knaff

Authors
  1. R. Max Wynn
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  2. Dale F. Gaul
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  3. Won-Ki Choi
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  4. Robert W. Shaw
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  5. David B. Knaff
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Additional information

Dedicated to Prof. L.N.M. Duysens on the occasion of his retirement.

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Wynn, R.M., Gaul, D.F., Choi, WK. et al. Isolation of cytochrome bc 1 complexes from the photosynthetic bacteria Rhodopseudomonas viridis and Rhodospirillum rubrum . Photosynth Res 9, 181–195 (1986). https://doi.org/10.1007/BF00029743

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  • DOI: https://doi.org/10.1007/BF00029743

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