Abstract
The influence of culture filtrates of Fusarium oxysporum f.sp. dianthi which causes Fusarium wilt was investigated on growth and viability of carnation tissue cultures and leaf segments. Culture filtrates of avirulent race 1 of this fungus did not affect calli and leaf segments of cultivars both susceptible and resistant to Fusarium wilt. However, culture filtrates of virulent race 2 decreased viability and suppressed growth of callus of the susceptible cultivar. In contrast, callus of the resistant cultivar showed resistance to the culture filtrates. The results of these experiments may provide information on methods of selection of new wilt resistant carnation varieties.
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Abbreviations
- A270:
-
absorbance at 270 nm
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- CF-MCD:
-
culture filtrate of 16064 grown in MCD medium
- MCD medium:
-
modified Czapeck-Dox medium
- MS medium:
-
basal medium of Murashige and Skoog
- MW:
-
molecular weight
- PD medium:
-
potato dextrose medium
- TTC:
-
2,3,5-triphenyl tetrazolium chloride
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Arai, M., Takeuchi, M. Influence of Fusarium wilt toxin(s) on carnation cells. Plant Cell Tiss Organ Cult 34, 287–293 (1993). https://doi.org/10.1007/BF00029718
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DOI: https://doi.org/10.1007/BF00029718