Abstract
Sorghum mitochondrialatp6 occurs as one copy in the line Tx398 and as two copies in IS1112C. In IS1112C a repeated sequence diverged within theatp6 open reading frames. The two open reading frames (1137 bp,atp6-1; 1002 bp,atp6-2) share an identical conserved region of 756 bp but are flanked 5′ by divergent extensions of 246 (atp6-1) or 381 bp (atp6-2). Tx398 carried onlyatp6-2. The breakpoint of the repeated sequence of the conserved core region corresponds to the amino acid sequence Ser-Pro-Leu-Asp, which is the amino terminus of the proteolytically processed yeast ATP6. The 5′ extensions ofatp6-1 andatp6-2 were similar to those of rice and maize, respectively. Each open reading is transcribed, however nuclear background influenced transcriptional patterns ofatp6-2 in IS1112C.
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Bland MM, Levings CSIII, Matzinger DF: The ATPase subunit 6 gene of tobacco mitochondria contains an unusual sequence. Curr Genet 12: 475–481 (1987).
Bonen L, Bird S: Sequence analysis of the wheat mitochondrialatp6 gene reveals a fused upstream reading frame and markedly divergent N termini among plant ATP6 proteins. Gene 73: 47–56 (1988).
Bonen L, Bird S, Belanger L: Characterization of the wheat mitochondrial orf25 gene Plant Mol Biol 15: 793–795 (1990).
Dewey RE, Levings CSIII, Timothy DH: Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol 79: 914–919 (1985).
Dewey RE, Levings CSIII, Timothy DH: Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm Cell 44: 439–449 (1986).
Dewey RE, Timothy DH, Levings CSIII: Chimeric mitochondrial genes expressed in the C male-sterile cytoplasm of maize. Curr Genet 20: 475–482 (1991).
Fragoso LL, Nichols SE, Levings CSIII: Rearrangements in maize mitochondrial genes. Genome 31: 160–168 (1989).
Grabau E, Havlik M, Gesteland R: Chimeric organization of two genes for the soybean mitochondrial ATPase subunit 6. Curr Genet 13: 83–89 (1988).
Kadowaki K, Suzuki T, Kazama S: A chimeric gene containing the 5′ portion ofatp6 is associated with cytoplasmic male-sterility of rice. Mol Gen Genet 224: 10–16 (1990).
Kempken F, Mullen JA, Pring DR, Tang HV: RNA editing of sorghum mitochondrialatp6 transcripts changes 15 amino acids and generates a carboxy terminus identical to yeast. Curr Genet 20: 417–422 (1991).
Kennell JC, Pring DR: Initiation and processing ofatp6, T-urf13 and ORF221 transcripts from mitochondria of T cytoplasm maize. Mol Gen Genet 216: 16–24 (1989).
Lonsdale DM, Hodge TP, Fauron CM-R: The physical map and oranization of the mitochondrial genome from the fertile cytoplasm of maize. Nucl Acids Res 12: 9249–9261 (1984).
Macfarlane JL, Wahleithner JA, Wostenholme DR: A broad bean mitochondrialatp6 gene with an unusually simple, non-conserved 5′ region. Curr Genet 18: 87–91 (1990).
Macino G, Tzagoloff A: Assembly of the mitochondrial membrane system: sequence analysis of a yeast mitochondrial ATPase gene containing the oli-2 and oli-4 loci. Cell 20: 507–517 (1980).
Makaroff CA, Apel IJ, Palmer JD: The atp6 coding region has been disrupted and a novel reading frame generated in the mitochondrial genome of cytoplasmic male sterile radish. J Biol Chem 264: 11706–11713 (1989).
Michon T, Galante M, Velours J: NH2-terminal sequence of the isolated yeast ATP synthase subunit 6 reveals posttranslational cleavage. Eur J Biochem 172: 621–625 (1988).
Pring DR, Gengenbach BG, Wise RP: Recombination is associated with polymorphism of the mitochondrial genomes of maize and sorghum. Phil Trans R Soc Lond B 319: 187–198 (1988).
Schuster W, Brennicke A: Nucleotide sequence of the Oenothera ATPase subunit 6 gene. Nucl Acids Res 15: 9092 (1987).
Stamper SE, Dewey RE, Bland MM, Levings CSIII: Characterization of the gene urf13-T and an unidentified reading frame, ORF 25, in maize and tobacco mitochondria. Curr Genet 12: 457–463 (1987).
Worstell JV, Kidd HJ, Scherts KF: Relationships among male-sterility inducing cytoplasms of sorghum. Crop Sci 24: 186–189 (1984).
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Mullen, J.A., Pring, D.R., Kempken, F. et al. Sorghum mitochondrialatp6: divergent amino extensions to a conserved core polypeptide. Plant Mol Biol 20, 71–79 (1992). https://doi.org/10.1007/BF00029150
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DOI: https://doi.org/10.1007/BF00029150