Skip to main content
Log in

Sorghum mitochondrialatp6: divergent amino extensions to a conserved core polypeptide

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

Sorghum mitochondrialatp6 occurs as one copy in the line Tx398 and as two copies in IS1112C. In IS1112C a repeated sequence diverged within theatp6 open reading frames. The two open reading frames (1137 bp,atp6-1; 1002 bp,atp6-2) share an identical conserved region of 756 bp but are flanked 5′ by divergent extensions of 246 (atp6-1) or 381 bp (atp6-2). Tx398 carried onlyatp6-2. The breakpoint of the repeated sequence of the conserved core region corresponds to the amino acid sequence Ser-Pro-Leu-Asp, which is the amino terminus of the proteolytically processed yeast ATP6. The 5′ extensions ofatp6-1 andatp6-2 were similar to those of rice and maize, respectively. Each open reading is transcribed, however nuclear background influenced transcriptional patterns ofatp6-2 in IS1112C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Bland MM, Levings CSIII, Matzinger DF: The ATPase subunit 6 gene of tobacco mitochondria contains an unusual sequence. Curr Genet 12: 475–481 (1987).

    PubMed  Google Scholar 

  2. Bonen L, Bird S: Sequence analysis of the wheat mitochondrialatp6 gene reveals a fused upstream reading frame and markedly divergent N termini among plant ATP6 proteins. Gene 73: 47–56 (1988).

    Article  PubMed  Google Scholar 

  3. Bonen L, Bird S, Belanger L: Characterization of the wheat mitochondrial orf25 gene Plant Mol Biol 15: 793–795 (1990).

    Article  PubMed  Google Scholar 

  4. Dewey RE, Levings CSIII, Timothy DH: Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol 79: 914–919 (1985).

    Google Scholar 

  5. Dewey RE, Levings CSIII, Timothy DH: Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm Cell 44: 439–449 (1986).

    Article  PubMed  Google Scholar 

  6. Dewey RE, Timothy DH, Levings CSIII: Chimeric mitochondrial genes expressed in the C male-sterile cytoplasm of maize. Curr Genet 20: 475–482 (1991).

    Article  PubMed  Google Scholar 

  7. Fragoso LL, Nichols SE, Levings CSIII: Rearrangements in maize mitochondrial genes. Genome 31: 160–168 (1989).

    Google Scholar 

  8. Grabau E, Havlik M, Gesteland R: Chimeric organization of two genes for the soybean mitochondrial ATPase subunit 6. Curr Genet 13: 83–89 (1988).

    PubMed  Google Scholar 

  9. Kadowaki K, Suzuki T, Kazama S: A chimeric gene containing the 5′ portion ofatp6 is associated with cytoplasmic male-sterility of rice. Mol Gen Genet 224: 10–16 (1990).

    Article  PubMed  Google Scholar 

  10. Kempken F, Mullen JA, Pring DR, Tang HV: RNA editing of sorghum mitochondrialatp6 transcripts changes 15 amino acids and generates a carboxy terminus identical to yeast. Curr Genet 20: 417–422 (1991).

    Article  PubMed  Google Scholar 

  11. Kennell JC, Pring DR: Initiation and processing ofatp6, T-urf13 and ORF221 transcripts from mitochondria of T cytoplasm maize. Mol Gen Genet 216: 16–24 (1989).

    Article  Google Scholar 

  12. Lonsdale DM, Hodge TP, Fauron CM-R: The physical map and oranization of the mitochondrial genome from the fertile cytoplasm of maize. Nucl Acids Res 12: 9249–9261 (1984).

    PubMed  Google Scholar 

  13. Macfarlane JL, Wahleithner JA, Wostenholme DR: A broad bean mitochondrialatp6 gene with an unusually simple, non-conserved 5′ region. Curr Genet 18: 87–91 (1990).

    Article  PubMed  Google Scholar 

  14. Macino G, Tzagoloff A: Assembly of the mitochondrial membrane system: sequence analysis of a yeast mitochondrial ATPase gene containing the oli-2 and oli-4 loci. Cell 20: 507–517 (1980).

    Article  PubMed  Google Scholar 

  15. Makaroff CA, Apel IJ, Palmer JD: The atp6 coding region has been disrupted and a novel reading frame generated in the mitochondrial genome of cytoplasmic male sterile radish. J Biol Chem 264: 11706–11713 (1989).

    PubMed  Google Scholar 

  16. Michon T, Galante M, Velours J: NH2-terminal sequence of the isolated yeast ATP synthase subunit 6 reveals posttranslational cleavage. Eur J Biochem 172: 621–625 (1988).

    PubMed  Google Scholar 

  17. Pring DR, Gengenbach BG, Wise RP: Recombination is associated with polymorphism of the mitochondrial genomes of maize and sorghum. Phil Trans R Soc Lond B 319: 187–198 (1988).

    Google Scholar 

  18. Schuster W, Brennicke A: Nucleotide sequence of the Oenothera ATPase subunit 6 gene. Nucl Acids Res 15: 9092 (1987).

    PubMed  Google Scholar 

  19. Stamper SE, Dewey RE, Bland MM, Levings CSIII: Characterization of the gene urf13-T and an unidentified reading frame, ORF 25, in maize and tobacco mitochondria. Curr Genet 12: 457–463 (1987).

    PubMed  Google Scholar 

  20. Worstell JV, Kidd HJ, Scherts KF: Relationships among male-sterility inducing cytoplasms of sorghum. Crop Sci 24: 186–189 (1984).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mullen, J.A., Pring, D.R., Kempken, F. et al. Sorghum mitochondrialatp6: divergent amino extensions to a conserved core polypeptide. Plant Mol Biol 20, 71–79 (1992). https://doi.org/10.1007/BF00029150

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00029150

Key words

Navigation