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Thecab-m7 gene: a light-inducible, mesophyll-specific gene of maize

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Abstract

Southern blot analysis has revealed the existence in maize of perhaps 12 members of the nuclearcab multigene family encoding the chlorophylla- andb-binding proteins of the photosystem II light-harvesting complex. Hybridization with 3′ probes derived from unsequenced cDNA clones showed that six members of this family differ from one another with respect to expression in mesophyll and/or bundle sheath cells and regulation by light. An additional member of this family, designatedcab-m7, that encodes a 28 kDa primary translation product has now been identified. It has been cloned from a maize genomic library and sequenced to begin to define the bases for differences in the expression of these genes. Thiscab gene is shown to be strongly preferentially expressed in the mesophyll (vs. bundle sheath) cells of maize. Furthermore, the gene is photo-responsive; although small amounts ofcab-m7 mRNA are present in etiolated leaves, the mRNA pool is 8-fold larger after six hours of illumination. DNA sequences upstream of thecab-m7 gene resemble those found in the 5′-flanking regions of some other plant genes.

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Author notes

  1. Thomas W. Becker

    Present address: Institut National de la Recherche Agronomique, F-78026, Versailles, France

  2. Thomas S. Templeman

    Present address: Gene Track Systems, 31 New York Ave., 01701, Framingham, MA, USA

Authors and Affiliations

  1. Department of Cellular and Developmental Biology, Harvard University, 16 Divinity Avenue, 02138, Cambridge, MA, USA

    Thomas W. Becker, Thomas S. Templeman, Jean-Frédéric Viret & Lawrence Bogorad

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  1. Thomas W. Becker
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  2. Thomas S. Templeman
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  3. Jean-Frédéric Viret
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  4. Lawrence Bogorad
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Becker, T.W., Templeman, T.S., Viret, JF. et al. Thecab-m7 gene: a light-inducible, mesophyll-specific gene of maize. Plant Mol Biol 20, 49–60 (1992). https://doi.org/10.1007/BF00029148

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  • DOI: https://doi.org/10.1007/BF00029148

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