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Comparison between the organization of nuclear ribosomal DNA unit of Euglena gracilis Z and var. Bacillaris

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Summary

We have characterized the nuclear rDNA unit of Euglena gracilis var. bacillaris and compared it to that of the Z strain. We have localized restriction sites for Eco R1, Sal 1, Sma 1, Hind III, Bam H1 and Bgl II on this unit as well as the coding region for 20 S and 25 S rRNAs. For both strains, results suggest an homogeneity of the 11.6 kbp rDNA units. Comparison between strains shows differences characterized by two additional Sal 1 sites in bacillaris and the likely methylation of one Sma 1 site in Z. Both differences are localized in a non-coding region of the rDNA unit. Analyses of 18 Euglena strains from various origins confirm these differences and allow easy recognition of bacillaris and Z type strains.

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Abbreviations

kb:

kilo base

kpb:

kilo base pair

plasmids:

pRH 59 and pRH 57 contain a Hind III-HInd III nuclear DNA fragment from W3BUL of 5.9 and 5.7 kbp respectively, pRB 48 and pRB 35 contain a Bam H1-Bam H1 nuclear DNA fragment from wild-type Z of 4.8 and 3.5 kbp respectively

SDS:

sodium dodecyl sulfate

UV:

ultra-violet

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Author notes

  1. Odile Neyret-Djossou

    Present address: Centre de Recherche, UNICET, BP 11, 69572, Dardilly Cédex, France

Authors and Affiliations

  1. Groupe de Recherche en Biochimie-Biologie Cellulaire, Université de limoges, 87060, Limoges Cédex, France

    Odile Neyret-Djossou & Georges Freyssinet

  2. Applications Laboratoire de Biologie Expérimentale, Université de Limoges, 87060, Limoges Cédex, France

    Odile Neyret-Djossou & Georges Freyssinet

  3. Département de Biologie Générale et Appliquée, UA CNRS 92, Université Lyon I, 69622, Villeurbanne Cédex, France

    Patrick Ravel-Chapuis & Philippe Heizmann

Authors
  1. Odile Neyret-Djossou
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  2. Georges Freyssinet
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  3. Patrick Ravel-Chapuis
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  4. Philippe Heizmann
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Neyret-Djossou, O., Freyssinet, G., Ravel-Chapuis, P. et al. Comparison between the organization of nuclear ribosomal DNA unit of Euglena gracilis Z and var. Bacillaris . Plant Mol Biol 6, 111–117 (1986). https://doi.org/10.1007/BF00027304

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  • DOI: https://doi.org/10.1007/BF00027304

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