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Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China

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Abstract

Filamentous Bangia, which are distributed extensively throughout the world, have simple and similar morphological characteristics. Scientists can classify these organisms using molecular markers in combination with morphology. We successfully sequenced the complete nuclear ribosomal DNA, approximately 13 kb in length, from a marine Bangia population. We further analyzed the small subunit ribosomal DNA gene (nrSSU) and the internal transcribed spacer (ITS) sequence regions along with nine other marine, and two freshwater Bangia samples from China. Pairwise distances of the nrSSU and 5.8S ribosomal DNA gene sequences show the marine samples grouping together with low divergences (00.003; 0–0.006, respectively) from each other, but high divergences (0.123–0.126; 0.198, respectively) from freshwater samples. An exception is the marine sample collected from Weihai, which shows high divergence from both other marine samples (0.063–0.065; 0.129, respectively) and the freshwater samples (0.097; 0.120, respectively). A maximum likelihood phylogenetic tree based on a combined SSU-ITS dataset with maximum likelihood method shows the samples divided into three clades, with the two marine sample clades containing Bangia spp. from North America, Europe, Asia, and Australia; and one freshwater clade, containing Bangia atropurpurea from North America and China.

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Authors and Affiliations

  1. Department of Cell Biology, College of Biology and Basic Medical Sciences, Soochow University, SIP, Suzhou, 215123, China

    Jiajie Xu  (徐佳杰), Yuan He  (何渊) & Songdong Shen  (沈颂东)

  2. Department of Biology and Food Engineering, Changshu Institute of Technology, Changshu, 215500, China

    Bo Jiang  (姜波), Jianyi Zhu  (朱建一) & Zonggen Shen  (沈宗根)

  3. Maternal and Child Health Hospital of Gansu Province, Lanzhou, 730050, China

    Sanming Chai  (柴三明)

Authors
  1. Jiajie Xu  (徐佳杰)
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  2. Bo Jiang  (姜波)
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  3. Sanming Chai  (柴三明)
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  4. Yuan He  (何渊)
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  5. Jianyi Zhu  (朱建一)
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  6. Zonggen Shen  (沈宗根)
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  7. Songdong Shen  (沈颂东)
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Corresponding authors

Correspondence to Zonggen Shen  (沈宗根) or Songdong Shen  (沈颂东).

Additional information

Supported by the National Natural Science Foundation of China (Nos. 31270256, 41276134) and the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A406-6)

XU Jiajie and CHAI Sanming contributed equally to this work.

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Xu, J., Jiang, B., Chai, S. et al. Complete nuclear ribosomal DNA sequence amplification and molecular analyses of Bangia (Bangiales, Rhodophyta) from China. Chin. J. Ocean. Limnol. 34, 1044–1053 (2016). https://doi.org/10.1007/s00343-016-5033-1

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