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No direct linkage between proton pumping and photosynthate unloading from seed coats of Phaseolus vulgaris L.

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Abstract

The energization of the active sucrose release from bean seed-coat halves was investigated. For this purpose, seed coat tissues adjacent to the apoplastic space were exposed to a variety of treatments and proton and photosynthate release were measured. Fusicoccin (10−5 moll−1) stimulated proton pump activities. Orthovanadate (2×10−4 moll−1) and abscisic acid (10−5 moll−1) diminished the proton extrusion evoked by fusicoccin. Fusicoccin inhibited sucrose release, whereas orthovanadate and abscisic acid stimulated it. Addition of 100 mmoll−1 K+ had a promotory effect on photosynthate unloading, fading away with time. This extra unloading was linearly related to an enhanced proton loss. It was concluded that the photosynthate unloading apparently is not a proton/sucrose antiport and that a pump-leak system for photosynthate release is unlikely. A tentative model for photosynthate/proton symport not directly linked to proton pumping is presented as the mechanism of unloading.

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Abbreviations

ABA:

abscisic acid

CCCP:

carbonyl cyanide m-chlorophenyl hydrazone

DTE:

diethioerythritol

FC:

fusicoccin

MES:

2-(N′-morpholino) ethanesulfonic acid monohydrate

NEM:

n-ethylmaleimide

PCMBS:

p-chloromercuriphenylsulfonic acid

TRIS:

2-amino-2-(hydroxymethyl) propane-1,3 diol

VAN:

sodium orthovanadate

References

  1. Barbier H and Guern J (1981) Différence de potentiel de part et d'autre de la membrane vacuolaire et accumulation du saccharose chez Beta vulgaris L. CR Acad Sci Paris 292 III:785–788

    Google Scholar 

  2. Bennett AB and Spanswick RM (1983) Derepression of amino acid-H+ cotransport in developing soybean embryos. Pl Physiol 72:781–786

    Google Scholar 

  3. Cocucci M, Ballarin-Denti A and Marrè MT (1980) Effects of orthovanadate on H+ secretion, K+ uptake, electrical potential difference and membrane ATPase activities of higher plant tissues. Pl Sci Lett 17:391–400

    Google Scholar 

  4. Düring H and Alleweldt G (1980) Effects of plant hormones on phloem transport in grapevines. Ber Deutsch Bot Ges 93:339–347

    Google Scholar 

  5. Eschrich W (1980) Phloembeladung und verwandte Prozesse. Ber Deutsch Bot Ges 93:1–10

    Google Scholar 

  6. Gepstein S, Jacobs M and Taiz L (1982) Inhibition of stomatal opening in Vicia faba epidermal tissue by vanadate and abscisic acid. Pl Sci Lett 28:63–72

    Google Scholar 

  7. Guy M, Reinhold L and Michaeli D (1979) Direct evidence for a sugar transport mechanism in isolated vacuoles. Pl Physiol 64:61–64

    Google Scholar 

  8. Lado P, deMichelis MI, Cerana R and Marrè E (1976) Fusicoccin-induced K+-stimulated proton secretion and acid-induced growth of apical root segments. Pl Sci Lett 6:5–20

    Google Scholar 

  9. Lichtner FT and Spanswick RM (1981) Electrogenic sucrose transport in developing soybean cotyledons. Pl Physiol 67:869–874

    Google Scholar 

  10. Lichtner FT and Spanswick RM (1981) Sucrose uptake by developing soybean cotyledons. Pl Physiol 68:693–698

    Google Scholar 

  11. Marrè E (1979) Fusicoccin: a tool in plant physiology. Ann Rev Pl Physiol 30:273–288

    Google Scholar 

  12. Patrick JW (1981) An in vitro assay of sucrose uptake by developing bean cotyledons. Aust J Pl Physiol 8:221–235

    Google Scholar 

  13. Patrick JW (1983) Photosynthate unloading from seed coats of Phaseolus vulgaris L. General characteristics and facilitated transfer. Zeitsch Pflanzenphysiol 111:9–18

    Google Scholar 

  14. Patrick JW and McDonald R (1980) Pathway of carbon transport within developing ovules of Phaseolus vulgaris L. Aust J Pl Physiol 7:671–684

    Google Scholar 

  15. Tanner W (1980) On the possible role of ABA on phloem unloading. Ber Deutsch Bot Ges 93:349–351

    Google Scholar 

  16. Thorne JH and Rainbird RM (1983) An in vivo technique for the study of phloem unloading in seed coats of developing soybean seeds. Pl Physiol 72:268–271

    Google Scholar 

  17. Wolswinkel P and Ammerlaan A (1983) Phloem unloading in developing seeds of Vicia faba L. The effect of several inhibitors on the release of sucrose and amino acids by the seed coat. Planta 158:205–215

    Google Scholar 

  18. Wolswinkel P, Ammerlaan A and Kuyvenhoven H (1983) Effect of KCN and p-chloromercuri benzenesulfonic acid on the release of sucrose and 2-amino (1-14C) isobutyric acid by the seed coats of Pisum sativum. Physiol Plant 59: 375–386

    Google Scholar 

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Authors and Affiliations

  1. Botanisch Laboratorium, Rijksuniversiteit Utrecht, Lange Nieuwstraat 106, 3512 PN, Utrecht, The Netherlands

    A. J. E. Van Bel

  2. Department of Biological Sciences, University of Newcastle, 2308, NSW, Australia

    J. W. Patrick

Authors
  1. A. J. E. Van Bel
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  2. J. W. Patrick
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Van Bel, A.J.E., Patrick, J.W. No direct linkage between proton pumping and photosynthate unloading from seed coats of Phaseolus vulgaris L.. Plant Growth Regul 2, 319–326 (1984). https://doi.org/10.1007/BF00027291

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  • DOI: https://doi.org/10.1007/BF00027291

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