Summary
Week old seedlings of indica rice variety Jaya obtained on basal MS medium and further sub-cultured on agar solidified MS medium supplemented with cytokinins, sucrose (3% w/v) and mannitol (1% w/v) lead to development of multiple shoot buds. Shoot cultures were maintained and multiplied in liquid medium containing BAP 5 mg l-1, sucrose (3% w/v) and mannitol (1% w/v). Profuse rooting was obtained on transfer to MS liquid medium containing IBA 1 mg l-1 and sucrose (3% w/v). Complete plants were successfully transferred to soil and grown to maturity.
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References
Abdullah R., E.C.Cocking & J.A.Thompson, 1986. Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4: 1087–1090.
Abe T. & Y.Futsuhara, 1985. Efficient plant regeneration by somatic embryogenesis from root callus tissues of rice (Oryza sativa L.). J. Plant Physiol 121: 111–118.
Brar, D.S., D.H. Ling & S. Yoshida, 1985. Plant regeneration from somatic cell cultures of some IR varieties of rice. In: Biotechnology in International Agricultural Research, IRRI Pub., pp. 169–177.
Chen Y., Q.Zuo, L.S.D.Lu & K.L.Zheng, 1981. Green plant regeneration from isolated rice pollen grains in vitro and the induction factors. Acta Genet. Sci. 8: 158–163.
Datta S.K., K.Datta & T.Potrykus, 1990. Embryogenesis and plant regeneration from microspores of both indica and japonica rice (Oryza sativa). Plant Sci. 67 (1): 83–88.
Finch R.P., A.Baset, I.H.Slamet & E.C.Cocking, 1992. In vitro shoot culture of wild Orygae and other grass species. Plant Cell, Tissue and Organ Culture 30: 31–39.
Greco B., A.A.Lomonaco, B.Boggini, C.Tomassini & O.A.Tanzanella, 1990. Clonal propagation of rice through proliferation of axillary shoots. Euphytica 48 (2): 123–127.
Hisajima S., P.Chongpraditnum & Y.Arai, 1987. Microplant propagation of rice plant in vitro. Japan J. Trop. Agri. 31 (1): 12–15.
Kumari D.S., N.P.Sharma & G.J.N.Rao, 1988. Micropropagation of cytosterile rice stocks. Intl. Rice Res. New. L. 13: 5–6.
Murashige T. & F.Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 13: 473–479.
Peng H., H.Kononowicz & T.K.Hodges, 1992. Transgenic indica rice plants. Theor. Appl. Genet. 83: 855–863.
Yamada Y., Z.Q.Yang & D.T.Tang, 1986. Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep. 5 (NZ): 85–88.
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Sandhu, J.S., Gosal, S.S., Gill, M.S. et al. Micropropagation of Indica rice through proliferation of axillary shoots. Euphytica 81, 139–142 (1995). https://doi.org/10.1007/BF00025425
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DOI: https://doi.org/10.1007/BF00025425