Abstract
The binding constants of various olefins were determined with a Triton X-100 extract of mung bean sprouts. The olefins tested included compounds that have been reported to induce an ethylene response in vivo as well as olefins reported to block the ethylene response. Both types of compounds were bound by the Triton X-100 extract, and the binding constants in vitro were usually considerably lower than those obtained in in vivo studies as measured by gas phase concentrations. Increased solubility due to Triton X-100 solution appears to be partly responsible. The in vitro binding order of compounds reported to induce an ethylene response was similar to their order of in vivo activity. Also, the compounds which gave an anti-ethylene response in vivo bound to the extract in approximately the same order as their in vivo effectiveness. These results suggest that binding of olefins is not the only factor necessary for an ethylene response. Although binding is necessary for activity, another factor must be involved after binding, and this may be π-acceptance.
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Beggs MJ and Sisler EC (1983) Ethylene binding properties of mung bean extracts. Plant Physiol Suppl 72: S40
Bengochea T, Dodds JH, Evans DE, Jerie PH, Niepel B and Hall MA (1980) Studies on ethylene binding by cell-free preparations from cotyledons of Phaseolus vulgarus L. I. Separation and characterization. Planta 148: 397–406
Burg SP and Burg EA (1967) Molecular requirements for the biological activity of ethylene. Plant Physiol 42: 144–152
Cotton FA and Wilkinson G (1980) Advanced Inorganic Chemistry, p. 1199. New York: Wiley
Hartley FR (1973) Thermodynamic data for olefin and acetylene complexes of transition metals. Chem Rev 73: 163–190
McAuliffe C (1966) Solubility in water of paraffin, cycloparaffin, olefin, acetylene, cycloolefin and aromatic hydrocarbons. J Phys Chem 70: 1267–1275
Murray WJ, Hall LH and Kier LB (1975) Molecular connectivity III: Relationship to partition coefficients. J Pharm Sci 64: 1978–1981
Segel IH (1975) Enzyme Kinetics. 957 p. New York: Wiley-Interscience
Sisler EC (1977) Ethylene activity of some π-acceptor compounds. Tobacco Sci 21: 43–45
Sisler EC (1979) Measurement of ethylene binding in plant tissue. Plant Physiol 64: 538–542
Sisler EC (1980) Partial purification of an ethylene-binding component from plant tissue. Plant Physiol 66: 404–406
Sisler EC and Goren R (1981) Ethylene binding—the basis for hormone action in plants? What's New in Plant Physiol 12: 37–40
Sisler EC and Wylie PA (1978) Effect of some α-olefin derivatives in relation to the ethylene response. Tobacco Sci 22: 102–105
Sisler EC and Yang SF (1983) Effects of butenes and cyclic olefins on etiolated pea plants in relation to the ethylene response. Phytochemistry (in press)
Smith AR and Hall MA (1984) Mechanisms of ethylene action. Plant Growth Regul 2: 151–165
Smith AR, Howard CJ, Sanders IO and Hall MA (1984) The effect of cycloalkenes on ethylene binding and metabolism. In: Fuchs Y and Chalutz E, eds. Ethylene: Biochemical, Physiological and Applied Aspects, pp 99–100. The Hague: Nijhoff/Junk
Thompson JS, Harlow RL and Whitney JF (1984) Copper(I)-olefin complexes: Support for the proposed role of copper in the ethylene effect in plants. J Amer Chem Soc 105: 3522–3527
Trewavas AJ and Jones AM (1981) Consequences of hormone-binding. What's New in Plant Physiol 12: 5–8
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Beggs, M.J., Sisler, E.C. Binding of ethylene analogs and cyclic olefins to a Triton X-100 extract from plants: Comparison with in vivo activities. Plant Growth Regul 4, 13–21 (1986). https://doi.org/10.1007/BF00025345
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DOI: https://doi.org/10.1007/BF00025345