Abstract
Effects of metal chelators, 2,2′-bipyridine, 8-hydroxyquinoline and 1,10-phenenthroline, on the conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene in detached leaves of light-grown rice (Oryza sativa) seedlings and detached shoots of etiolated rice seedlings were investigated. Metal chelators strongly inhibited the in vivo ACC oxidase activity in detached leaves and detached etiolated shoots. This inhibition could be partially recovered by Fe2+. Our results support the notion that Fe2+ is an essential cofactor for the conversion of ACC to ethylene in vivo.
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Abbreviations
- ACC:
-
1-aminocyclopropane-1-carboxylic acid
- BP:
-
2,2′-bypyridine
- HQ:
-
8-hydroxylquinoline
- MJ:
-
methyl jasmonate
- PA:
-
1,10-phenanthroline
- Put:
-
putrescine
References
Apelbaum A, Burgoon AC, Anderson JD, Solomos T and Liberman M (1981) Some characteristics of the system converting 1-aminocyclopropane-1-carboxylic acid to ethylene. Plant Physiol 67: 80–84
Baldwin JE, Jackson DA, Adlington RW and Rawlings BJ (1985) The stereochemistry of oxidation of 1-aminocyclopropane-1-carboxylic acid. J Chem Soc Chem Commun 1985: 206
Boller T, Herner RC and Kende H (1979) Assay for and enzymatic formation of an ethylene precursor, 1-aminocyclopropane-1-carboxylic acid. Planta 145: 293–303
Bouzayen M, Felix G, Latche A, Pech JC and Boller T (1991) Iron: an essential cofactor for the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene. Planta 184: 244–247
Chen CT and Kao CH (1990) Comparative study of the metabolism of 1-aminocyclopropane-1-carboxylic acid and senescence of water-stressed and ABA-treated excised rice leaves. Plant Cell Physiol 31: 463–468
Chen CT, Chou IT and Kao CH (1990) Senescence of rice leaves XX. Changes of proton secretion during senescence. Plant Sci 66: 29–34
Chen SL, Chen CT and Kao CH (1991) Polyamines promote the biosynthesis of ethylene in detached rice leaves. Plant Cell Physiol 32: 813–817
Fernandez-Maculet JC and Yang SF (1992) Extraction and partial characterization of the ethylene-forming enzyme from apple fruit. Plant Physiol 99: 751–754
Kao CH and Yang SF (1982) Light inhibition of the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene in leaves is mediated through carbon dioxide. Planta 155: 261–266
Pirung MC (1983) Ethylene biosynthesis. 2. Stereochemistry of ripening, stress, and model reactions J Am Chem Soc 105: 7207–7209
Ververidis P and John P (1991) Complete recovery in vivo of ethylene-forming enzyme activity. Phytochemistry 30: 725–727
Yang SF and Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35: 155–189
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Tsai, FY., Kao, C.H. The effects of 2,2′-bipyridine and other metal chelators on the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene in rice. Plant Growth Regul 14, 145–149 (1994). https://doi.org/10.1007/BF00025216
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DOI: https://doi.org/10.1007/BF00025216