Skip to main content
SpringerLink
Log in

Effect of kinetin and GA3 on in vitro ovule embryo culture of Cucumis melo L.

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The ability of Cucumis melo embryos of different ages to form plants in vitro was studied in order to rescue hybrid embryos between C. melo and Cucumis metuliferus. Plants were grown in a glasshouse at temperatures ranging from 15°CN-28°CD. Best results were obtained with ovule embryos excised 17 days after pollination. At this age, kinetin of 0.5 mg l−1 was found optimal for culturing embryo development. Similar results were obtained with ovule embryos excised 14 days after pollination which cultured on 0.5 mg l−1 kinetin with 0.5 mg l−1 GA3.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Beharav A and Cohen Y (1994) The crossability of Cucumis melo and C. metuliferus, an investigation of in vivo pollen tube growth. Cuc Genet Coop 17: 97–100

    Google Scholar 

  2. Chatterjee M and More TA (1991) Techniques to overcome barrier of interspecific hybridization in Cucumis. Cuc Genet Coop 14: 66–68

    Google Scholar 

  3. Custers JBM (1981) Heart shape stage embryos of Cucumis species more successful in embryo culture than advanced stage embryos. Cuc Genet Coop 4: 48–49

    Google Scholar 

  4. Den Nijs APM, Custers JBM and Kooistra AJ (1980) Reciprocal crosses between Cucumis africanus and C. metuliferus Naud. I. Overcoming barriers to fertilization by mentor pollen and AVG. Cuc Genet Coop 3: 60–62

    Google Scholar 

  5. Fassuliotis G (1977) Self-fertilization of Cucumis metuliferus Naud. and its cross-compatibility with C. melo L. J Am Soc Hortic Sci 102: 336–339

    Google Scholar 

  6. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–479

    Google Scholar 

  7. Perring TM, Ferrar CA, Mayberry K and Blua MJ (1992) Research reveals pattern of cucurbit virus spread. Calif Agric 46: 35–40

    Google Scholar 

  8. Punja ZK, Tang FA and Watkins LH (1988) Identification of resistance to root knot nematodes and virus diseases in Cucumis metuliferus and approaches to hybridization with Cucumis sativus by protoplast fusion. Phytopathol 78 (Abstr)

  9. Rakoczy-Trojanowska M and Malepzy W (1986) Obtaining of hybrids within the family Cucrbitaceae. I. Characteristics of hybrids from Cucurbita maxima×C. pepo. Genet Pol 27: 259–272

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Life Sciences, Bar-Ilan University, 52900, Ramat Gan, Israel

    Alexander Beharav & Yigal Cohen

Authors
  1. Alexander Beharav
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yigal Cohen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Beharav, A., Cohen, Y. Effect of kinetin and GA3 on in vitro ovule embryo culture of Cucumis melo L.. Plant Growth Regul 16, 267–269 (1995). https://doi.org/10.1007/BF00024785

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00024785

Key words

Search

Navigation