Abstract
Plant-derived smoke extracts stimulate the germination of many different seeds. the present report explains steps to determine some of the chemical characteristics of the compounds concerned. Grand Rapids lettuce seeds were used as a bioassay because smoke-derived extracts overcome their light-sensitivity. The active compounds were partitioned into ethyl acetate, separated by various TLC systems and fractionated by reverse phase HPLC. They are stable surviving a series of chromatographic steps and are very active biologically. In order to ascertain their chemical properties it was necessary to use a range of dilutions after each isolation step. It would appear that similar types of compounds are present in smoke extracts derived from different plant material.
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van Staden, J., Drewes, F.E. & Brown, N.A.C. Some chromatographic characteristics of germination stimulants in plant-derived smoke extracts. Plant Growth Regul 17, 241–249 (1995). https://doi.org/10.1007/BF00024732
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DOI: https://doi.org/10.1007/BF00024732