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Calcium-mediated changes in peroxidase and O-diphenol oxidase activities of cotton fibres (Gossypium spp.) and its possible relationship to ABA

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Abstract

This work is an investigation of the influence of ABA and calcium on soluble and wall-bound activities of peroxidase and O-diphenol oxidase of cotton fibres at the stages of primary and secondary wall development, during incubation of intact fibres for 3h at 28°C. ABA (10 μM) caused marked inhibition of enzyme activities in both the fractions, whereas calcium (1 mM) was promotory. The incorporation of 1 mM EGTA (a calcium chelator) and chlorpromazine (10 μg cm−3) (a calmodulin antagonist) resulted in decreased enzyme activities suggesting regulation of enzyme synthesis and/or secretion to the cell wall by calcium-calmodulin. As a general trend, the relative effect of Ca2+ on the activity of peroxidase in the wall was much greater than on the soluble activity, but this was not true of O-diphenol oxidase. It is inferred that ABA inhibits enzymic activities by inhibiting calmodulin synthesis or its mobilization to sites of action.

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Abbreviations

ABA:

abscisic acid

EGTA:

ethylene glycol-bis(β amino-ethyl ether)N,N′ tetraacetic acid

DAA:

days after anthesis

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Authors and Affiliations

  1. Department of Botany, Punjab Agricultural University, 141004, Ludhiana, India

    A. S. Basra, R. S. Sarlach, Rewa Dhillon-Grewal & C. P. Malik

Authors
  1. A. S. Basra
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  2. R. S. Sarlach
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  3. Rewa Dhillon-Grewal
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  4. C. P. Malik
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Basra, A.S., Sarlach, R.S., Dhillon-Grewal, R. et al. Calcium-mediated changes in peroxidase and O-diphenol oxidase activities of cotton fibres (Gossypium spp.) and its possible relationship to ABA. Plant Growth Regul 11, 159–164 (1992). https://doi.org/10.1007/BF00024070

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  • DOI: https://doi.org/10.1007/BF00024070

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