Abstract
The effect of increased concentrations of calcium (Ca) (3–24 mM) and boron (B) (100–800 μM) in the medium was studied on the occurrence of shoot tip necrosis (STN) in cultures of Pistacia vera L. STN was significantly reduced by application of Ca or B, however media with more than 200 μM boron had reduced shoot multiplication. Ca (12–24 mM) supplied as calcium chloride reduced STN without any adverse effect on shoot multiplication or elongation, whereas calcium acetate reduced elongation. It is concluded that STN is a physiological mineral disorder associated with Ca and/or B deficiency in the meristematic regions of actively growing shoots. Application of Ca (up to 24 mM) as calcium chloride to the medium was the best treatment for the control of STN. Reduction of humidity or increased aeration in the culture jars did not have any significant effect on the occurrence of STN.
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Abbreviations
- MS:
-
Murashige and Skoog medium
- STN:
-
Shoot tip necrosis
References
Adams P and Winsor GW (1974) Some responses of glasshouse tomatoes to boron. J Hort Sci 49: 355–363
Barghchi M and Alderson PG (1983) In vitro propagation of Pistacia vera L. from seedling tissues. J Hort Sci 58: 435–445
Barghchi M and Alderson PG (1985) In vitro propagation of Pistacia vera L. and commercial varieties of ‘Ohadi’ and “Kalleghochi”. J Hort Sci 60: 423–430
Barghchi M and Alderson PG (1989) Pistachio (Pistacia vera L.), In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol. 5, Trees II. Berlin-Heidelburg-New York: Springer Verlag
Biddulph O, Nakayama FS and Cory R (1961) Transpiration stream and ascension of calcium. Plant Physiol 36: 429–436
Borchert R (1986) Calcium acetate induced calcium uptake and formation of calcium-oxalate crystals in isolated leaflets of Gleditsia triacanthos L. Planta 571–578
Bowen JE (1972) Effect of environmental factors on water utilisation and boron accumulation and translocation in sugar cane. Plant and Cell Physiol 13: 703–714
Carbonell J and Jones RL (1984) A comparison of the effects of Ca2+ and gibberellic acid an enzyme synthesis and secretion in barley aleurone. Physiologia P1 63: 345–350
Eaton FM (1940) Interrelations in the effect of boron and indole acetic acid on plant growth. Bot Gaz 101: 700–705
Edwards JH, Reeves DW, Horton BD and Thompson JM (1985) Influence of Ca concentration on growth, tissue concentration, and nutrient uptake of in vitro propagated plums and lovell seedlings. J Plant Nut 8: 327–344
Geraldson CM (1954) The control of blackheart of celery. Proc Ameri Soc hort Sci 63: 353–358
Kohle H, Jeblick W, Poten F, Blaschek W and Kauss H (1985) Chiltosan-elicited callose synthesis in soybean cells as a Ca2+ dependent process. Plant Physiol 77: 344–551
Kohl HC and Oertli JJ (1961) Distribution of boron in leaves. Plant Physiol 36: 420–424
Kotze WAG (1979) Ionic interactions in the uptake and transport of calcium by apple seedlings. Communications in Soil Science and Plant Analysis 10: 115–127
Lee S and Aronoff S (1967) Boron in plants-a biochemical role. Science 158: 798–799
Levi E (1968) The distribution of mineral elements following leaf and root uptake. Physiologia P1 21: 213–226
Lovatt CJ (1985) Evolution of xylem resulted in a requirement for boron in the apical meristem of vascular plants. New Phytol 99: 509–522
Maynard DN, Gersten B, Vernell HF (1965) The distributions of calcium as related to internal tipburn, variety and calcium nutrition in cabbage. Proc Amer Soc hort Sci 86: 392–396
McHargue JS and Calfee RK (1932) Effect of boron on growth of lettuce. Plant Physiol 7: 161–164
McLlrath WJ and Skok J (1964) Boron nutrition and lignification in sunflower and tobacco stems. Bot Gaz 125: 268–271
Misaghi IJ and Grogan RG (1978) Physiological basis for tipburn development in head lettuce. Phytopathology 68: 1744–1753
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Pl 15: 473–497
Palzkill DA, Tibbitts TW and Williams PH (1976) Enhancement of calcium transport to inner leaves of cabbage for prevention of tipburn. J Amer Soc Hort Sci 101: 645–648
Parsa AA and Wallace A (1980) Differential partitioning of boron and calcium in shoots of seedlings of two pistachio cultivars. J Plant Nut 2: 263–266
Penel C, Sticher L, Kevers C, Gaspar T and Greppins H (1984) Calcium controlled peroxidase secretion by sugarbeet cells: effect of ionophores in relation to organogenesis. Biochem emi und Physiologie der Pflanzen 179: 173–180
Perkins HJ and Aronoff S (1956) Identification of the blue fluorescent compounds in boron deficient plants. Archives of Biochemistry and Biophysics 64: 506–507
Rajaratnam JA, Lowry JB, Avadhani PN and Corley R (1971) Boron: possible role in plant metabolism. Science 172: 1142–1143
Sepaskhah AR and Maftoun M (1982) Growth and chemical composition of pistachio seedlings as influenced by irrigation regimes and salinity levels of irrigation water. II. Chemical composition. J Hort Sci 57: 469–476
Sha L, McCown BH and Peterson L.A (1985) Occurrence and cause of shoot tip necrosis in shoot cultures. J Amer Soc hort Sci 110: 631–634
Shannon S, Natli JJ and Atkins JD (1967) Relation of calcium nutrition to hypocotyl necrosis of snap bean (Phaseolus vulgaris L.). Proc Amer Soc hort Sci 90: 180–190
Tromp J and Oele J (1972) Shoot growth and mineral composition of leaves and fruits as affected by relative air humidity. Physiologia Pl 27: 253–258
Wardle K, Dobbs EB and Short KC (1983) In vitro acclimatisation of aseptically cultured plantlets to humidity. J Amer Soc hort Sci 108: 386–389
Will G (1985) Nutrient deficiencies and fertiliser use in New Zealand exotic forest. FRI Bulletins, No 97, Rotorua: Forest Research Institute
Winsor G and Adams P (1987) Diagnosis of mineral disorders in plants. Glasshouse Crops, Vol 3, London: HMSO
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Barghchi, M., Alderson, P.G. The control of shoot tip necrosis in Pistacia vera L. in vitro . Plant Growth Regul 20, 31–35 (1996). https://doi.org/10.1007/BF00024054
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DOI: https://doi.org/10.1007/BF00024054