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The electrofocusing composition of the Large and Small Sub-unit polypeptides of Fraction-1-Protein in rice x wheat and rice x sorghum hybrids

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Summary

The polypeptide composition of Fraction-1-Protein (F1P) from rice × sorghum, rice × wheat hybrids and their respective parents have been analyzed by a microelectrofocusing method. The large sub-unit (LSU) is composed of three polypeptides and the small sub-unit (SSU) of two polypeptides in rice and sorghum parents and rice × sorghum hybrids. Similarly, LSU is composed of three polypeptides in the rice and wheat parents and rice × wheat hybrids. Two polypeptides occur in the SSU of rice parent and rice × wheat hybrids where as only one polypeptide in the wheat parent. These polypeptides also differ in their isoelectric points. Based on the previous reports of F1P inheritance in hybrids in other crops, F1P analysis of rice × sorghum and rice × wheat hybrids does not seem to be an important marker to identify such intergeneric hybrids. Since this is first such report of F1P inheritance in hybrids between distantly related plants, its implication in different modes of inheritance are discussed.

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Abbreviations

F1P:

Fraction-1-Protein

IEF:

Isoelectric focusing

pI:

Isoelectric points

LSU:

Large sub-unit

RuBPCase:

Ribulose 1,5-Bisphosphate Carboxylase-oxygenase

SSU:

Small sub-unit

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Author notes

  1. N. K. Srivastava

    Present address: Department of Plant Physiology and Biochemistry, Central Institute of Medicinal and Aromatic Plants, RSM Nagar, Post Bag No. 1, P.O., 226016, Lucknow, India

Authors and Affiliations

  1. Department of Plant Physiology, International Rice Research Institute, P.O. Box 933, Manila, The Philippines

    N. K. Srivastava & S. Yoshida

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  1. N. K. Srivastava
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  2. S. Yoshida
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Srivastava, N.K., Yoshida, S. The electrofocusing composition of the Large and Small Sub-unit polypeptides of Fraction-1-Protein in rice x wheat and rice x sorghum hybrids. Euphytica 48, 219–224 (1990). https://doi.org/10.1007/BF00023653

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  • DOI: https://doi.org/10.1007/BF00023653

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