Abstract
A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich proteins. The WPRP1 cDNA clone was used to determine the copy number and chromosomal location of the WPRP1 gene by restriction fragment length polymorphism analysis of wheat inbred lines. Although WPRP1 is encoded by a single-copy gene it is also a representative of a larger family of related sequences. RNA gel blot analysis showed that expression of WPRP1 is highest in rapidly growing tissue which together with its amino acid composition suggests a structural role for the encoded protein.
Similar content being viewed by others
References
Baulcombe DC, Buffard D: Gibberellic-acid-regulated expression of α-amylase and six other genes in wheat aleurone layers. Planta 157: 493–501 (1983).
Cassab GI, Varner JE: Cell wall proteins. Ann Rev Plant Physiol Plant Mol Biol 39: 321–353, (1988).
Chao S, Raines CA, Longstaff M, Sharp PJ, Gale MD, Dyer TA: Chromosomal location and copy number in wheat and some of its close relatives of genes for enzymes involved in photosynthesis. Mol Gen Genet 218: 423–430 (1989).
Chen J, Varner JE: An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin. EMBO J 4: 2145–2151 (1985).
Chen J, Varner JE: Isolation and characterisation of cDNA clones for carrot extensin and a proline-rich 33-kDa protein. Proc Natl Acad Sci USA 82: 4399–4403 (1985).
Condit CM, Meagher RB: A gene encoding a novel glycine-rich protein in petunia. Nature 323: 178–181 (1986).
Cooper JB, Chen JA, van Holst G-J, Varner JE: Hydroxyproline-rich glycoproteins of plant cell walls. TIBS 12: 24–27 (1987).
Corbin DR, Sauer N, Lamb CJ: Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants. Mol Cell Biol 7: 4337–4344 (1987).
Datta K, Schmidt A, Marcus A: Characterisation of two soybean repetitive proline-rich proteins and a cognate cDNA from germinated axes. Plant Cell 1: 945–952 (1989).
Dean C, Leech RM: Genome expression during normal leaf development. Plant Physiol 218: 904–910 (1982).
Devereux J, Haberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).
Feinberg AP, Vogelstein B: A technique for labelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).
Franssen HJ, Nap JP, Gloudemans T, Stiekema W, van Dam H, Govers F, Louwerse J, van Kammen A, Bisseling T: Characterization of a cDNA for nodulin-75 of soybean: A gene product involved in the early stages of root nodule development. Proc Natl Acad Sci USA 84: 4495–4499 (1987).
Henikoff S: Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28: 351–359 (1984).
Hong JC, Nagao RT, Key JL: Characterization and sequence analysis of a developmentally regulated putative cell wall protein gene isolated from soybean. J Biol Chem 262: 8367–8376 (1987).
Hong JC, Nagao RT, Key JL: Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell 1: 937–943 (1989).
Keller B, Lamb CJ: Specific expression of a novel cell wall hydroxyproline-rich glycoprotein gene in lateral root initiation. Genes Dev 3: 1639–1646 (1989).
Keller B, Sauer N, Lamb CJ: Glycine-rich cell wall proteins in bean: gene structure and association of the protein with the vascular system. EMBO J 7: 3625–3633 (1988).
Kieliszewski M, Lamport DTA: Purification and partial characterization of a hydroxyproline-rich glycoprotein in a graminaceous monocot, Zea mays. Plant Physiol 85: 823–827 (1987).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).
Memelink J, Hoge JHC, Schilperoort RA: Cytokinin stress changes the developmental regulation of several defence-related genes in tobacco. EMBO J 6: 3579–3583 (1987).
Murphy G, Kavanagh TA: Speeding-up the sequencing of double stranded DNA. Nucl Acids Res 16: 5198 (1988).
Raines CA, Lloyd JC, Longstaff M, Bradley D, Dyer TA: Chloroplast fructose-1,6-bisphosphatase: the product of a mosaic gene. Nucl Acids Res 16: 7931–7942 (1988).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Sharp PJ, Kreis M, Shewry PR, Gale MD: The location of α-amylase sequences in wheat and its relatives. Theor Appl Genet 75: 286–290 (1988).
Showalter AM, Bell JN, Cramer CL, Bailey JA, Varner JE, Lamb CJ: Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection. Proc Natl Acad Sci USA 82: 6551–6555 (1985).
Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517 (1975).
Stiefel V, Perez-Grau L, Alberticio F, Giralt E, Ruiz-Avila L, Ludevid MD, Puigdomenech P: Molecular cloning of cDNAs encoding a putative cell wall protein from Zea mays and immunological identification of related polypeptides. Plant Mol Biol 11: 483–493 (1988).
Varner JE, Lin L-S: Plant cell wall architecture Cell 56: 231–239 (1989).
Woessner JP, Goodenough UW: Molecular characterization of a zygote wall protein: an extensin-like molecule in Chlamydomonas reinhardtii. Plant cell 1: 901–911 (1989).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Raines, C.A., Lloyd, J.C., Chao, S. et al. A novel proline-rich protein from wheat. Plant Mol Biol 16, 663–670 (1991). https://doi.org/10.1007/BF00023430
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00023430