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Sequence of the iaa and ipt region of different Agrobacterium tumefaciens biotype III octopine strains: reconstruction of octopine Ti plasmid evolution

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Abstract

The TA regions of biotype III octopine/cucumopine (OC) Ti plasmids are closely related to the TL region of the biotype I octopine Ti plasmids pTiAch5 and pTi15955. Sequence analysis shows that the limited and wide host range biotype III OC TA regions are derived from a common ancestor structure which lacked the 6a gene found in the biotype I octopine TL region. The TA region of the wide host range OC Ti plasmids has conserved most of the original TL-like structure. In most wide host range OC isolates the TA-iaaH gene is inactivated by the insertion of an IS866 element. However, the TA region of the wide host range isolate Hm1 carries an intact TA-iaaH gene. This gene encodes a biologically active product, as shown by root induction tests and indole-3-acetic acid measurements.

The limited host range OC Ti plasmids pTiAB3 and pTiAg57 have shorter TA regions which are derived from a wide host range TA region. The AB3 type arose by an IS868-mediated, internal TA region deletion which removed the iaa genes and part of the ipt gene and left a copy of IS868 at the position of the deleted fragment. The pTiAB3 iaa/ipt deletion was followed by insertion of a second IS element, IS869, immediately 3′ of the ipt gene. pTiAg57 underwent the same iaa-ipt deletion as pTiAB3, but lacks the IS868 and IS869 elements.

Analysis of the various TA region structures provides a detailed insight into the evolution of the biotype III OC strains.

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  1. Institut de Biologie Moléculaire des Plantes du C.N.R.S., 12, Rue du Général Zimmer, 67084, Strasbourg, France

    Francois Paulus, Jean Canaday, Florence Vincent, Géraldine Bonnard, Christa Kares & Leon Otten

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  1. Francois Paulus
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  2. Jean Canaday
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  3. Florence Vincent
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  4. Géraldine Bonnard
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  5. Christa Kares
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  6. Leon Otten
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Paulus, F., Canaday, J., Vincent, F. et al. Sequence of the iaa and ipt region of different Agrobacterium tumefaciens biotype III octopine strains: reconstruction of octopine Ti plasmid evolution. Plant Mol Biol 16, 601–614 (1991). https://doi.org/10.1007/BF00023425

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  • DOI: https://doi.org/10.1007/BF00023425

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