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Inoculation with ascospores of Sclerotinia trifoliorum for detection of clover rot resistant red clover

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Abstract

Breeding red clover for resistance to clover rot by mycelial inoculation presents many difficulties. It was investigated whether an other method of infection, viz. ascospore inoculation, would yield better results.

From the investigations the following conclusions can be drawn:

  1. 1.

    Raising apothecia on a large scale and inoculating clover plants with the ascospores is a simple procedure.

  2. 2.

    Ascospore dispersion can be stimulated by allowing a cold air current over the ripe apothecia.

  3. 3.

    The degree of susceptibility of red clover varieties cannot be inferred from the density of brown spots in the leaves, caused with ascospore infection.

  4. 4.

    One field experiment with varieties and families, and an experiment in a chamber and in a frame, the latter two with varieties only, did not demonstrate that ascospore inoculation revealed the resistance better than the customary mycelial infection. By operating in a chamber or in a frame the rotting in clover necessary for selection purposes, can be produced in the properest way be mycelial inoculation. In the open a combination of mycelial and ascospore inoculation is recommended because under these circumstances there is a greater chance that the desired degree of rotting occurs.

  5. 5.

    With mycelial inoculation some varieties appeared to possess an other susceptibility to clover rot than when ascospore inoculation was applied. A possible explanation of this phenomenon was given.

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  1. Foundation for Agricultural Plant Breeding, Wageningen, Netherlands

    J. Dijkstra

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  1. J. Dijkstra
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Dijkstra, J. Inoculation with ascospores of Sclerotinia trifoliorum for detection of clover rot resistant red clover. Euphytica 13, 314–329 (1964). https://doi.org/10.1007/BF00023112

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  • DOI: https://doi.org/10.1007/BF00023112

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