Summary
A comparative test of six inoculation methods was conducted using 2 halo blight race 2 virulent strains, Nebr. HB 16 and HB 21 (Pseudomonas syringae pv. phaseolicola), on five dry bean cultivars/lines (Phaseolus vulgaris L.) of known resistance and susceptibility. The water-soaking of leaves method caused the most severe reaction among the leaf inoculation methods, followed by the carborundum, spraying and multiple needle methods, respectively. The seed soaking method was considered too severe to be useful, since entries identified as resistant by the other methods, were susceptible with the former method. Great Northern Nebraska # 1 sel. 27 and PI 150414 had the highest level of leaf resistance, but the former developed systemic chlorosis with the stem stabbing method, but not the latter line. No systemic chlorosis was seen in either line with the other methods of inoculation. This suggests that there may be a different genetic mechanism conferring resistance/susceptibility to the toxin in these two lines when the stabbing method is used. No interaction occurred between method by genotype and isolate by method but significant interactions occurred between genotype by isolate and method by isolate by genotype. The leaf and pod reaction of forty cultivars/lines to the new halo blight Nebr. Charlevoix strain was also determined. Different combinations of degrees of resistance and susceptibility of leaves and pods were observed. GN Tara, GN Harris, and PI 150414 had the highest combination of leaf and pod resistance.
Similar content being viewed by others
References
Andrus, C. F., 1948. A method of testing beans for resistance to bacterial blights. Phytopathology 38: 757–759.
Arp, G., D. P.Coyne & M. L.Schuster, 1971. Disease reaction of bean varieties to Xanthomonas phaseoli and Xanthomonas phaseoli var. fuscans using two inoculation methods. Plant Dis. Reptr. 55: 577–579.
Bullard, E. T., 1946. Breeding for halo blight resistance in beans. M.S. Thesis, Cornell University, Ithaca, N.Y., USA.
Burkholder, W. H., 1930. The bacterial diseases of the bean. A comparative study. Cornell Univ. Agr. Expt. Sta. Memoir 127: 1–88.
Butcher, Clyde L., L. L.Dean & L.LaFerriere, 1967. Incidence of halo blight in Idaho. Plant Dis. Reptr. 51: 310–311.
Coyne, D. P. & M. L.Schuster, 1974. Breeding and genetic studies of tolerance to several bean (Phaseolus vulgaris L.) bacterial pathogens. Euphytica 23: 651–656.
Coyne, D. P. & M. L.Schuster, 1979. Bacterial diseases of legumes: Breeding and resistance, pp. 225–233. In: R. J.Summerfield & A. H.Bunting (Eds), Advances in legume science. Royal Botanical Garden, Kew, Surrey, U.K.
Goth, R. W., 1966. The use of a partial vacuum to inoculate bean seed with pathogenic bacteria. Plant Dis. Reptr. 50: 110–111.
Hill, K., D. P.Coyne & M. L.Schuster, 1972. Leaf. pod and systemic chlorosis reaction in Phaseolus vulgaris to halo blight controlled by different genes. J. Amer. Soc. Hort. Sci. 97: 494–498.
Hubbeling, N., 1957. New aspects of breeding for disease resistance in beans. Euphytica 6: 111–141.
Hubbeling, N., 1973. Field resistance in Phaseolus beans to Pseudomonas phaseolicola. Meded. Fakult. Landboww., Gent. 38: 1351–1363.
Patel, P. N. & J. C.Walker, 1965. Resistance in Phaseolus to halo blight in beans. Phytopathology 56: 681–682.
Schuster, M. L., 1955. A method of testing resistance of beans to bacterial blights. Phytopathology 45: 519–520.
Schuster, M. L. & D. P.Coyne, 1975. Genetic variation in bean bacterial pathogens. Euphytica 24: 143–147.
Schuster, M. L. & D. P.Coyne, 1981. Biology, epidemiology, genetics and breeding for resistance to bacterial pathogens of Phaseolus vulgaris L. Hort. Reviews 3: 28–57.
Schuster, M. L., D. P.Coyne & E. D.Kerr, 1966. Pathogenicity tests of Nebraska isolates of halo blight bacteria on legumes. Ann. Reptr. Bean Improv. Coop. 9: 35–36.
Schwartz, H. F., 1980. Miscellaneous bacterial diseases, pp. 173–194. In: H. F.Schwartz & G. E.Galvez (Eds). Bean production problems. Centro Internacional de Agricultura Tropical (CIAT), CALI, Colombia, South America.
Snedecor, G. W. & W. G.Cochran, 1981. Statistical methods. Iowa State Univ. Press, Ames, Iowa, pp. 135–148.
Zaiter, Haytham, Z., 1982. Testing inoculation methods and sources of resistance to halo blight bacterium (Pseudomonas phaseolicola) in Phaseolus vulgaris. M. S. Thesis, Univ. Nebraska, Lincoln, Nebraska, US. pp. 1–62.
Zaumeyer, W. J. & Thomas, H. R. 1957. A monographic study of bean diseases and methods for their control. U.S. Dept. of Agric. Tech. Bull. 868, pp. 74–84.
Author information
Authors and Affiliations
Additional information
Published as paper No. 7094, Journal Series, Nebraska Agricultural Experiment Station. Research was conducted under Project No. 20-036.
Rights and permissions
About this article
Cite this article
Zaiter, H.Z., Coyne, D.P. Testing inoculation methods and sources of resistance to the halo blight bacterium (Pseudomonas syringae pv. phaseolicola) in Phaseolus vulgaris . Euphytica 33, 133–141 (1984). https://doi.org/10.1007/BF00022759
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00022759