Summary
In cabbage (Brassica oleracea var. capitata) and Chinese kale (B. oleracea alboglabra) four types of N-bands can be distinguished: pericentromeric, telomeric (terminal), intercalary and satellite bands. Typical NOR bands were not observed. The pericentromeric bands appear at the pericentric regions, possibly even at the centromeres of all chromosomes. Telomeric bands are observed on the short arms of chromosomes 1,5 and 6 in cabbage and chromosomes 1 and 5 in Chinese kale. Intercalary bands stained weakly in the long arms of chromosome 3 in cabbage and chromosome 2 in Chinese kale. Satellite bands cover the entire satellites in both Brassica species. The N-banding pattern is very similar in appearance to the C-banding pattern in both species and much more convenient to apply.
Similar content being viewed by others
References
Ahmad, Q.N., E.J. Britten & D.E. Byth, 1983. A quantitative method of karyotypic analysis applied to the soybean, Glycine max. Cytologia 48: 879–892.
Armstrong, K.C. 1982. N-banding in Triticum aestivum following Feulgen hydrolysis. Theor. Appl. Genet. 61: 337–339.
Chen, R.-Y., W.-Q. Song & X.-L. Li, 1981. Studies on cultivate barley (Hordeum vulgare) chromosome banding pattern. Acta Botanica Sinica 23: 162–165.
Funaki, K., S-I. Matsui & M. Sasaki, 1975. Location of nucleolar organizers in animal and plant chromosomes by means of an improved N-banding technique. Chrom. 49: 357–370.
Berlach, W.L., 1977. N-banding karyotypes of wheat species. Chrom. 62: 49–56.
Kurata, N. & T. Omura, 1978. Karyotype analysis in rice I. a new method for identifying all chromosome pairs. Japan. J. Genet. 53: 251–255.
Levan, A., K. Fredga & A.A. Sandberg, 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.
Matsui, S-I., 1973. Differential staining of nucleolus organizers in mammalian chromosomes. Nature 246: 148–150.
Matsui, S-I., 1974. Nucleolus organizer of Vicia faba chromosomes revealed by the N-banding technique. Japan. J. Genet. 47: 93–96.
Mouras, A., 1975. Sur l'utilisation des protoplasts en caryologie: application au dénombrement chromosomique des cultures de tissus de Tabac. C.R. Acad. Sc. Paris, 281, Série D: 255–258.
Mouras, A., G. Salesses & A. Lutz, 1978. Sur l'utilisation des protoplasts en cytologie: Amélioration d'une méthode récente en vue de l'identification des chromosomes mitotiques des genres Nicotiana et Prunus. Caryologia 31: 117–127.
Schlegel, R. & B.S. Gill, 1984. N-banding analysis of rye chromosomes and the relationship between N-banded and C-banded heterochromatin. Can. J. Genet. Cytol. 26: 765–796.
Wang, X.-H. & P. Luo, 1987. Studies on the karyotypes and C-banding patterns of Chinese kale and cabbage. Acta Botanica Sinica 29: 149–155.
Zhang, Z.-L., G.-Y. Dong & P. Lu, 1982. Study on the chromosome N-banding in plants. Acta Botanica Sinica 24: 120–124.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, X.H., Luo, P. & Shu, J.J. Giemsa N-banding pattern in cabbage and Chinese kale. Euphytica 41, 17–21 (1989). https://doi.org/10.1007/BF00022406
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00022406