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Cloning and initial characterization of 14 myb-related cDNAs from tomato (Lycopersicon esculentum cv. Ailsa Craig)

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Abstract

myb-related transcription factors contain highly conserved DNA-binding domains. Using a mixture of degenerate oligonucleotides derived from the highly conserved region as probe, 14 myb-related clones were isolated from a cDNA library constructed using tomato hypocoyl mRNA. The expression of these clones was studied by northern blot analysis using poly(A)+ RNA from 7 tissue types (hypocotyl, leaf, root, green and red fruit, immature and mature flower). This study has revealed a wide range of expression patterns which include multiple and single transcripts, some of which show marked tissue specificity. Two clones showing different expression patterns have been fully sequenced. The DNA-binding domains of these two tomato myb clones are compared with myb genes from other plant species and organisms. Of the three clones analysed so far by Southern hybridization, two are single-copy genes and one has multiple genomic copies.

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  1. Quan Lin

    Present address: John Innes Centre, Norwich Research Park, Colney, NR4 7UJ, Norwich, UK

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  1. Axis Genetics Ltd., Babraham, CB2 4AZ, Cambridge, UK

    Quan Lin, William D. O. Hamilton & Andrew Merryweather

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Lin, Q., Hamilton, W.D.O. & Merryweather, A. Cloning and initial characterization of 14 myb-related cDNAs from tomato (Lycopersicon esculentum cv. Ailsa Craig). Plant Mol Biol 30, 1009–1020 (1996). https://doi.org/10.1007/BF00020811

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  • DOI: https://doi.org/10.1007/BF00020811

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