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A novel glycine-rich cell wall protein gene in rice

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Abstract

A genomic clone isolated from rice (Oryza sativa var. IR 36) contains a gene that encodes a glycine-rich cell wall protein. This gene is a member of a multi-gene family. Two transcripts with different 5′ termini are encoded by this gene and the expression of these two transcripts is differentially regulated. The amino acid sequence derived from the DNA sequence of this gene has a putative amino terminal signal peptide, and a highly repetitive structure in the putative mature protein. A model is proposed which describes the potential monomeric structure of this glycine-rich protein and how these monomers may be covalently linked to produce a network-like structure.

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References

  1. Averyhart-Fullard V, Datta K, Marcus A: A new hydroxyproline-rich protein in the soybean cell wall. Proc Natl Acad Sci USA 85: 1082–1085 (1988).

    PubMed  Google Scholar 

  2. Bensi G, Raugei G, Klefenz H, Cortese R: Structure and expression of the human haptoglobin locus. EMBO J 4: 119–126 (1985).

    PubMed  Google Scholar 

  3. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254 (1976).

    Article  PubMed  Google Scholar 

  4. Cassab GI, Varner JE: Cell wall proteins. Ann Rev Plant Physiol 349: 321–353 (1988).

    Article  Google Scholar 

  5. Chen JA, Varner JE: An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin. EMBO J 4: 2145–2151 (1985).

    Google Scholar 

  6. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5403 (1979).

    PubMed  Google Scholar 

  7. Condit CM, Meagher RB: A gene encoding a novel glycine-rich structural protein of petunia. Nature 323: 178–181 (1986).

    Google Scholar 

  8. Condit CM, Meagher RB: Expression of a gene encoding a glycine-rich protein in petunia. Mol Cell Biol 7: 4273–4279 (1987).

    PubMed  Google Scholar 

  9. Corbin DR, Sauer N, Lamb CJ: Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants. Mol Cell Biol 7: 4337–4344 (1987).

    PubMed  Google Scholar 

  10. deOliverira DE, Seurinck J, Inze D, VanMontagu M, Botterman: Differential expression of five Arabidopsis genes encoding glycine-rich proteins. Plant Cell 2: 427–436 (1990).

    Article  PubMed  Google Scholar 

  11. Ecker JR, Davis RW: Plant defense genes are regulated by ethylene. Proc Natl Acad Sci USA 84: 5202–5206 (1987).

    Google Scholar 

  12. Epstein L, Lamport DTA: An intramolecular linkage involving isodityrosine in extensin. Phytochemistry 23: 1241–1246 (1984).

    Article  Google Scholar 

  13. Esau K: Anatomy of seed plants. Wiley, New York, NY (1977).

    Google Scholar 

  14. Fry SC: Cross-linking of matrix polymers in the growing cell walls of angiosperms. Ann Rev Plant Physiol 37: 165–186 (1986).

    Google Scholar 

  15. Garnier J, Osguthorpe DJ, Robson B: Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol 120: 97–120 (1978).

    PubMed  Google Scholar 

  16. Gomez J, Sanchez-Martinez D, Stiefel V, Rigau J, Puigdomenech P, Pages M: A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature 334: 262–264 (1988)

    Article  PubMed  Google Scholar 

  17. Goodwin TW, Mercer EI: Introduction to Plant Biochemistry. Pergamon Press, New York, NY (1983).

    Google Scholar 

  18. Hong JC, Nagao RT, Key JL: Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell 1: 937–943.

  19. Hood EE, Shen QX, Varner JE: A developmentally regulated hydroxyproline-rich glycoprotein in maize pericarp cell walls. Plant Physiol 87: 138–142 (1988).

    Google Scholar 

  20. Keller B, Lamb CJ: Specific expression of a novel cell wall hydroxyproline-rich glycoprotein gene in lateral root initiation. Genes Dev 3: 1639–1646 (1989).

    PubMed  Google Scholar 

  21. Keller B, Sauer N, Lamb CJ: Glycine-rich cell wall proteins in bean: gene structure and association of the protein with the vascular system. EMBO J 7: 3625–3633 (1988).

    PubMed  Google Scholar 

  22. Keller B, Smid J, Lamb CJ: Vascular expression of a bean cell wall glycine-rich protein-β-glucuronidase gene fusion in transgenic tobacco. EMBO J 8: 1309–1314 (1989).

    Google Scholar 

  23. Keller B, Templeton MD, Lamb CJ: Specific localization of a plant cell wall glycine rich protein in protoxylem cells of the vascular system. Proc Natl Acad Sci USA 86: 1529–1533 (1989).

    Google Scholar 

  24. Kieliszewski M, Lamport DTA: Purification and partial characterization of a hydroxyproline-rich glycoprotein in a graminaceous monocot, Zea mays. Plant Physiol 85: 823–827 (1987).

    Google Scholar 

  25. Kieliszewski M, Leykam JF, Lamport DTA: Structure of the threoninerich extensin from Zea mays. Plant Physiol 92: 316–326 (1990).

    Google Scholar 

  26. Lamport DTA: The protein component of primery cell walls. Adv Bot Res 15: 151–218 (1965).

    Google Scholar 

  27. Lamport DTA: Structure, biosynthesis and significance of cell wall glycoproteins. In: Loewus FA, Runeckles VC (eds) Recent Advances in Phytochemistry Vol II. Plenum Press, New York (1977).

    Google Scholar 

  28. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1982).

    Google Scholar 

  29. Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR: Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl Acids Res 12: 7035–7056 (1984).

    PubMed  Google Scholar 

  30. Messing J, Crea R, Seeburg PH: A system for shotgun DNA sequencing. Nucl Acids Res 9: 309–321 (1982).

    Google Scholar 

  31. Moon E, Kao TH, Wu R: Rice chloroplast DNA molecules are heterogeneous as revealed by DNA sequences of a cluster of genes. Nucl Acids Res 15: 611–630 (1987).

    PubMed  Google Scholar 

  32. Mundy J, Chua NH: Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J 7: 2279–2286 (1988).

    PubMed  Google Scholar 

  33. Pauling L, Corey RB: Configurations of polypeptide chains with favored orientations around single bonds: two new pleated sheets. Proc Natl Acad Sci USA 37: 729–740 (1951).

    Google Scholar 

  34. Reddy ASN, Poovaiah BH: Accumulation of a glycine rich protein in auxin-deprived strawberry fruits. Biochem Biophys Res Commun 147: 885–891 (1987).

    PubMed  Google Scholar 

  35. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    PubMed  Google Scholar 

  36. Showalter AM, Bell JM, Cramer CL, Bailey JA, Varner JE: Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection. Proc Natl Acad Sci USA 82: 6551–6555 (1985).

    Google Scholar 

  37. Stiefel V, Peres-Grau L, Albericio F, Giralt E, Ruiz-Avila L, Ludevid MD, Puigdomenech P: Molecular cloning of cDNAs encoding a putative cell wall protein from Zea mays and immunological identification of related polypeptides. Plant Mol Biol 11: 483–493 (1988).

    Google Scholar 

  38. VanHolst GJ, Varner JE: Reinforced polyproline II conformation in a hydroxyproline-rich cell wall glycoprotein from carrot root. Plant Physiol 74: 247–251 (1984).

    Google Scholar 

  39. Varner JE, Lin LS: Plant cell wall architecture. Cell 56: 231–239 (1989).

    Article  PubMed  Google Scholar 

  40. Varner JE, Cassab GI: A new protein in petunia. Nature 323: 110 (1986).

    PubMed  Google Scholar 

  41. VonHeijne G: Transcending the impenetrable: How proteins come in terms with membranes. Biochim Biophys Acta 947: 307–333 (1988).

    PubMed  Google Scholar 

  42. Wolfe KH, Gouy M, Yang YW, Sharp PM, Li WH: Date of the monocotdicot divergence estimated from chloroplast DNA sequence data. Proc Natl Acad Sci USA 86: 6201–605 (1989).

    PubMed  Google Scholar 

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Lei, M., Wu, R. A novel glycine-rich cell wall protein gene in rice. Plant Mol Biol 16, 187–198 (1991). https://doi.org/10.1007/BF00020551

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  • DOI: https://doi.org/10.1007/BF00020551

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