Abstract
A genomic clone isolated from rice (Oryza sativa var. IR 36) contains a gene that encodes a glycine-rich cell wall protein. This gene is a member of a multi-gene family. Two transcripts with different 5′ termini are encoded by this gene and the expression of these two transcripts is differentially regulated. The amino acid sequence derived from the DNA sequence of this gene has a putative amino terminal signal peptide, and a highly repetitive structure in the putative mature protein. A model is proposed which describes the potential monomeric structure of this glycine-rich protein and how these monomers may be covalently linked to produce a network-like structure.
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Averyhart-Fullard V, Datta K, Marcus A: A new hydroxyproline-rich protein in the soybean cell wall. Proc Natl Acad Sci USA 85: 1082–1085 (1988).
Bensi G, Raugei G, Klefenz H, Cortese R: Structure and expression of the human haptoglobin locus. EMBO J 4: 119–126 (1985).
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254 (1976).
Cassab GI, Varner JE: Cell wall proteins. Ann Rev Plant Physiol 349: 321–353 (1988).
Chen JA, Varner JE: An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin. EMBO J 4: 2145–2151 (1985).
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5403 (1979).
Condit CM, Meagher RB: A gene encoding a novel glycine-rich structural protein of petunia. Nature 323: 178–181 (1986).
Condit CM, Meagher RB: Expression of a gene encoding a glycine-rich protein in petunia. Mol Cell Biol 7: 4273–4279 (1987).
Corbin DR, Sauer N, Lamb CJ: Differential regulation of a hydroxyproline-rich glycoprotein gene family in wounded and infected plants. Mol Cell Biol 7: 4337–4344 (1987).
deOliverira DE, Seurinck J, Inze D, VanMontagu M, Botterman: Differential expression of five Arabidopsis genes encoding glycine-rich proteins. Plant Cell 2: 427–436 (1990).
Ecker JR, Davis RW: Plant defense genes are regulated by ethylene. Proc Natl Acad Sci USA 84: 5202–5206 (1987).
Epstein L, Lamport DTA: An intramolecular linkage involving isodityrosine in extensin. Phytochemistry 23: 1241–1246 (1984).
Esau K: Anatomy of seed plants. Wiley, New York, NY (1977).
Fry SC: Cross-linking of matrix polymers in the growing cell walls of angiosperms. Ann Rev Plant Physiol 37: 165–186 (1986).
Garnier J, Osguthorpe DJ, Robson B: Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol 120: 97–120 (1978).
Gomez J, Sanchez-Martinez D, Stiefel V, Rigau J, Puigdomenech P, Pages M: A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature 334: 262–264 (1988)
Goodwin TW, Mercer EI: Introduction to Plant Biochemistry. Pergamon Press, New York, NY (1983).
Hong JC, Nagao RT, Key JL: Developmentally regulated expression of soybean proline-rich cell wall protein genes. Plant Cell 1: 937–943.
Hood EE, Shen QX, Varner JE: A developmentally regulated hydroxyproline-rich glycoprotein in maize pericarp cell walls. Plant Physiol 87: 138–142 (1988).
Keller B, Lamb CJ: Specific expression of a novel cell wall hydroxyproline-rich glycoprotein gene in lateral root initiation. Genes Dev 3: 1639–1646 (1989).
Keller B, Sauer N, Lamb CJ: Glycine-rich cell wall proteins in bean: gene structure and association of the protein with the vascular system. EMBO J 7: 3625–3633 (1988).
Keller B, Smid J, Lamb CJ: Vascular expression of a bean cell wall glycine-rich protein-β-glucuronidase gene fusion in transgenic tobacco. EMBO J 8: 1309–1314 (1989).
Keller B, Templeton MD, Lamb CJ: Specific localization of a plant cell wall glycine rich protein in protoxylem cells of the vascular system. Proc Natl Acad Sci USA 86: 1529–1533 (1989).
Kieliszewski M, Lamport DTA: Purification and partial characterization of a hydroxyproline-rich glycoprotein in a graminaceous monocot, Zea mays. Plant Physiol 85: 823–827 (1987).
Kieliszewski M, Leykam JF, Lamport DTA: Structure of the threoninerich extensin from Zea mays. Plant Physiol 92: 316–326 (1990).
Lamport DTA: The protein component of primery cell walls. Adv Bot Res 15: 151–218 (1965).
Lamport DTA: Structure, biosynthesis and significance of cell wall glycoproteins. In: Loewus FA, Runeckles VC (eds) Recent Advances in Phytochemistry Vol II. Plenum Press, New York (1977).
Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1982).
Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR: Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl Acids Res 12: 7035–7056 (1984).
Messing J, Crea R, Seeburg PH: A system for shotgun DNA sequencing. Nucl Acids Res 9: 309–321 (1982).
Moon E, Kao TH, Wu R: Rice chloroplast DNA molecules are heterogeneous as revealed by DNA sequences of a cluster of genes. Nucl Acids Res 15: 611–630 (1987).
Mundy J, Chua NH: Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J 7: 2279–2286 (1988).
Pauling L, Corey RB: Configurations of polypeptide chains with favored orientations around single bonds: two new pleated sheets. Proc Natl Acad Sci USA 37: 729–740 (1951).
Reddy ASN, Poovaiah BH: Accumulation of a glycine rich protein in auxin-deprived strawberry fruits. Biochem Biophys Res Commun 147: 885–891 (1987).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Showalter AM, Bell JM, Cramer CL, Bailey JA, Varner JE: Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection. Proc Natl Acad Sci USA 82: 6551–6555 (1985).
Stiefel V, Peres-Grau L, Albericio F, Giralt E, Ruiz-Avila L, Ludevid MD, Puigdomenech P: Molecular cloning of cDNAs encoding a putative cell wall protein from Zea mays and immunological identification of related polypeptides. Plant Mol Biol 11: 483–493 (1988).
VanHolst GJ, Varner JE: Reinforced polyproline II conformation in a hydroxyproline-rich cell wall glycoprotein from carrot root. Plant Physiol 74: 247–251 (1984).
Varner JE, Lin LS: Plant cell wall architecture. Cell 56: 231–239 (1989).
Varner JE, Cassab GI: A new protein in petunia. Nature 323: 110 (1986).
VonHeijne G: Transcending the impenetrable: How proteins come in terms with membranes. Biochim Biophys Acta 947: 307–333 (1988).
Wolfe KH, Gouy M, Yang YW, Sharp PM, Li WH: Date of the monocotdicot divergence estimated from chloroplast DNA sequence data. Proc Natl Acad Sci USA 86: 6201–605 (1989).
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Lei, M., Wu, R. A novel glycine-rich cell wall protein gene in rice. Plant Mol Biol 16, 187–198 (1991). https://doi.org/10.1007/BF00020551
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DOI: https://doi.org/10.1007/BF00020551