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Segregation of T-DNA copies in the progeny of a regenerant plant from a mannopine-positive hairy root line

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Abstract

We have analyzed opine content, T-DNA content, and developmental features of one hairy root culture line and its progeny. Opine-positive progeny still have the hairy root phenotype and have essentially the same T-DNA structure as the parental plant. Opineless progeny do not exhibit the hairy root phenotype. Some of them do not contain any T-DNA sequences, whereas others contain only the left part of T-DNA. These results are compatible with the hypothesis that, in the hairy root line analyzed, the left part of T-DNA is integrated independently from the core T-DNA and can therefore segregate during sexual reproduction.

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Authors and Affiliations

  1. Groupe de recherche sur les interactions entre microorganismes et plantes, Génétique et amélioration des plantes, INRA, Bat.409, F-91405, Orsay Cedex 05, France

    C. David & J. Tempé

  2. Institut de Microbiologie, UA 136, CNRS Faculté des sciences, Bat.409, F-91405, Orsay Cedex 05, France

    C. David & J. Tempé

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  1. C. David
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  2. J. Tempé
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David, C., Tempé, J. Segregation of T-DNA copies in the progeny of a regenerant plant from a mannopine-positive hairy root line. Plant Mol Biol 9, 585–592 (1987). https://doi.org/10.1007/BF00020535

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  • DOI: https://doi.org/10.1007/BF00020535

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