Abstract
Mutational analysis of Pisum sativum L. was used to search for constitutive proteins that might function in nodule formation. The sym 5 locus is a mutational hot spot, represented by seven independently derived mutant lines with decreased nodulation. Comparison of two-dimensional polyacrylamide gels of in vitro-translated root RNA showed a consistent difference in the migrational pattern of one peptide. In the nodulating parental cultivar ‘Sparkle’, a 66 kDa peptide had a pI of 5.9. In four of the five tested sym 5 mutants, the 66 kDa peptide had a more acidic pI of 5.8. This 66 kDa peptide is found in lateral root, tap root, and shoot. Its expression was independent of rhizobial inoculation, root temperature, or light.
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Fearn, J.C., LaRue, T.A. An altered constitutive peptide in sym 5 mutants of Pisum sativum L.. Plant Mol Biol 14, 207–216 (1990). https://doi.org/10.1007/BF00018561
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DOI: https://doi.org/10.1007/BF00018561