Abstract
The antenna complexes from Rps. cryptolactis have been isolated and purified. Rps. cryptolactis contains two types of variable antenna complex, B800-850 and B800-820 as well as the ‘core’ B875 antenna complex. The variable antenna complexes contain more than two types of antenna apoprotein, and have a Bchla:carotenoid ratio of ∼2:1. They can both be crystallised, but the B800-820 complex is the easiest with which to get relatively large single 3-D crystals (up to 0.5 mm in each dimension).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Angerhofer A, Cogdell RJ and Hipkins MF (1986) A spectral characterisation of the light-harvesting pigment-protein from Rps. acidophila. Biochim Biophys Acta 848: 833–841
Clayton RK (1964) Absorption spectra of photosynthetic bacteria and their chlorophylls. In: Gest N, San Pietro A and Vernon LP (eds) Bacterial Photosynthesis, pp 495–500. Antioch Press, Yellow Springs
Cogdell RJ and Crofts AR (1978) Analysis of the pigment content of an antenna pigment-protein complex from three strains of Rhodopseudomonas sphaeroides. Biochim Biophys Acta 502: 409–416
Cogdell RJ and Scheer H (1985) Circular dichroism of bacterial light-harvesting complexes. Photochem. Photobiol. 42: 669–689
Cogdell RJ, Durant I, Valentine J, Lindsay JG, and Schmidt K (1983) The isolation and partial characterisation of the light-harvesting pigment-protein complement of Rhodopseudomonas acidophila. Biochim Biophys Acta 722: 427–455
Cogdell RJ, Hawthornthwaite AM, Ferguson LA, Evans MB, Li M Gardiner A, Mackenzie RC, Thornber JP, Brunisholz RA, Zuber H, vanGrondelle R and vanMourik (1990) The structure and function of some unusual variable antenna complexes. In: Drews G and Davies EA (eds) Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria, pp 211–218. Plenum Press, New York
Cogdell RJ, Woolley KJ, Ferguson LA and Dawkins DJ (1991) Crystallisation of purple bacterial antenna complexes. In: Michel H (ed) CRC Handbook on Crystallisation of Membrane Proteins, pp 125–136. CRC Press, Boca Raton
Dawkins DJ (1988) Isolation and characterisation of RC-antenna conjugates from a range of Bchla containing species of purple bacteria. PhD Thesis, Univ of Glasgow, UK
Evans MB, Cogdell RJ and Britton G (1988) Determination of the Bchla: carotenoid ratios of the B890 antenna complex of Rhodospirillum rubrum and the B800–850 complex of Rhodobacter sphaeroides. Biochim Biophys Acta 935: 292–298
Evans MB, Hawthornthwaite AM and Cogdell RJ (1990) Isolation and characterisation of the different B800–850 light-harvesting complexes from low- and high-light grown cells of Rhodopseudomonas palustris strain 2.1.6. Biochim Biophys Acta 1026: 71–76
Garavito RM and Rosenbusch JP (1980) Three-dimensional crystals of an integral membrane protein: An initial X-ray analysis. J Cell Biol 86: 327–329
Gardiner AT, Cogdell RJ and Takaichi S (1993) The effect of growth conditions on the light-harvesting apparatus in Rhodopseudomonas acidophila. Photosynth Res 38: 159–167
Hayashi H, Miyao M and Morita S (1982) Absorption and fluorescence spectra of light-harvesting Bchl-protein complexes from Rhodopseudomonas palustris in the near infra red region. J Biochem 91: 1017–1027
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Michel H and Oesterhelt D (1980) Three-dimensional crystals of membrane proteins; bacteriorhodopsin. Proc Natl Acad Sci USA 77: 1283–1285
Papiz MZ, Hawthornthwaite AM, Cogdell RJ, Woolley KJ, Wightman PA, Ferguson LA and Lindsay JG (1989) Crystallisation of the B800–850 light-harvesting complex from Rhodopseudomonas acidophila strain 10050 and determination of its oligomeric state. J Mol Biol 209: 833–835
Schägger H and vonJagow G (1987) Tricine-SDS PAGE for the separation of protein in the range from 1–100 KDa. Anal Biochem 166: 368–379
Stadtwald-Demchick R, Turner RF and Gest H (1990) Rhodopseudomonas cryptolactis sp. nov. a new thermotolerant species of budding phototrophic purple bacteria. FEMS Microbiol Lett 71: 117–122
Tadros MH and Waterkamp (1989) Multiple copies of the coding regions for the light-harvesting B800–850 α- and β-polypeptides are present in the Rhodopseudomonas palustris genome. EMBO J 8: 1303–1308
Thornber JP (1970) Photochemical reactions of purple bacteria as revealed by studies of three spectrally different caroteno-bacteriochlorophyll-protein complexes isolated from Chromatium strain D. Biochemistry 9: 2688–2698
Zuber H (1990) Consideration on the structural principles of the antenna complexes of phototrophic bacteria. In: Drews G and Davies EA (eds) Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria, pp 161–180. Plenum Press, New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Halloren, E., McDermott, G., Lindsay, J.G. et al. Studies on the light-harvesting complexes from the thermotolerant purple bacterium Rhodopseudomonas cryptolactis . Photosynth Res 44, 149–155 (1995). https://doi.org/10.1007/BF00018305
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00018305