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Selective extraction of 22 kDa and 10 kDa polypeptides from Photosystem II without removal of 23 kDa and 17 kDa extrinsic proteins

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Abstract

Selective solubilization of Photosystem II membranes with the non-ionic detergent octyl thioglucopyranoside has allowed the isolation of a PS II system which has been depleted of the 22 and 10 kDa polypeptides but retains all three extrinsic proteins (33, 23 and 17 kDa). The PS II membranes which have been depleted of the 22 and 10 kDa species show high rates of oxygen evolution activity, external calcium is not required for activity and the manganese complex is not destroyed by exogenous reductants. When we compared this system to control PS II membranes, we observed a minor modification of the reducing side, and a conversion of the high-potential to the low-potential form of cytochrome b 559.

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Abbreviations

Chl-:

chlorophyll

DCBQ-:

2,5-dichloro-p-benzoquinone

DCMU-:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

ESR-:

electron spin resonance

MES-:

2-(N-morpholino)ethanesulfonic acid

OTG-:

octyl-β-d-thioglucopyranoside

PS II-:

Photosystem II

PEG-:

polyethylene glycol, Mr=6000

Tris-:

2-amino-2-hydroxyethylpropane-1,3-diol

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Authors and Affiliations

  1. Department of Chemistry, University of Crete, Iraklion, Crete, Greece

    Ranjit K. Mishra & Demetrios F. Ghanotakis

  2. Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Iraklion, Crete, Greece

    Ranjit K. Mishra & Demetrios F. Ghanotakis

Authors
  1. Ranjit K. Mishra
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  2. Demetrios F. Ghanotakis
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Mishra, R.K., Ghanotakis, D.F. Selective extraction of 22 kDa and 10 kDa polypeptides from Photosystem II without removal of 23 kDa and 17 kDa extrinsic proteins. Photosynth Res 36, 11–16 (1993). https://doi.org/10.1007/BF00018070

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  • DOI: https://doi.org/10.1007/BF00018070

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