Abstract
Chloroplast DNA was isolated and cloned from Chlorella, strain N1a, exsymbiotic with Paramecium bursaria. BamHI, SalI, SstI, KpnI and XhoI restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains only a single copy of the rRNA cistron. The rRNA cistron is small, 5000–8000 bp, and the 16S and 23S genes are separated by less than 2000 bp.
This is a preview of subscription content, log in via an institution to check access.
References
Bedbrook JR, Kolodner R, Bogorad L: Zea mays chloroplast ribosomal RNA genes are part of a 22,000 base pair inverted repeat. Cell 11: 739–749 (1977).
Gillham NW, Boynton, J, Harris, EH: Evolution of plastid DNA. In: Cavalier-Smith T (ed) The Evolution of Genome Size, pp. 299–351. John Wiley and Sons (1985).
Hedberg MF, Huang Y, Hommersand MH: Size of the chloroplast genome in Codium fragile. Science 231: 445–447b (1981).
Hanahan D: Techniques for transformation of E. coli In: Glover DM (ed) DNA Cloning: A Practical Approach, Vol. 1, pp. 109–135 IRL Press, Oxford (1985).
Kollar B, Delius H: Vicia faba chloroplast DNA has only one set of ribosomal RNA genes as shown by partial denaturation mapping and R-loop analysis. Mol Gen Genet 178: 261–269 (1980).
Kolodner R, Tewari KK: Inverted repeats in chloroplast DNA from higher plants. Proc Natl Acad Sci USA 76: 41–45 (1979).
Kuhsel M, Kowallik KV: The plastome of a brown alga, Dictyota dichotoma. Plant Mol Biol 4: 365–376 (1985).
Li N, Cattolico RA: Chloroplast genome characterization in the red alga, Griffithsia pacifica. Mol Gen Genet 209: 343–351 (1987).
Lidholm J, Szmidt AE, Hallgren J, Gustafsson P: The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats. Mol Gen Genet 212: 6–10 (1988).
Manhart JR, Kelly K, Dudock BS, Palmer JD: Universal characteristics of Codium fragile chloroplast DNA revealed by physical and gene mapping. Mol Gen Genet 216: 417–421 (1989).
Nichols HW, Bold MC: Trichosarchina polymorpha gen. et. sp. nov. J Phycol 1: 34–38 (1965).
Palmer JD: Comparative organization of chloroplast genomes. Ann Rev Gen 19: 325–354 (1985).
Palmer JD: Isolation and structural analysis of chloroplast DNA variation. Meth Enzymol 118: 167–186 (1986).
Palmer JD: Chloroplast evolution and biosystematic uses of chloroplast DNA variation. American Naturalist 30: S6-S29 (1987).
Rawson JRY, Kushner SR, Vapnek D, Alton NK, Boerma CL: Chloroplast ribosomal DNA genes in Euglena gracilis exist as three clustered tandem repeats. Gene 3: 191–209 (1978).
Tymms MY, Schweiger HG: Tandemly repeated nonribosomal DNA sequences in the chloroplast genome of an Acetabularia mediterranea strain. Proc Natl Acad Sci USA 82 1706–1710 (1985).
VanEtten JL, Burbank DE, Joshi J, Meints RH: DNA synthesis in a Chlorella-like alga following infection with the virus PBCV-1. Virology 134: 443–449 (1984).
VanEtten JL, Schuster AM, Girton L, Burbank DE, Swinton D, Hattman S: DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga. Nucl Acids Res 13: 3471–3478 (1985).
Waddle JA, Schuster AM, Lee KW, Meints RH: The mitochondrial genome of an exsymbiotic Chlorella-like green alga. Plant Mol Biol (in press).
Yamada T: Isolation and characterization of chloroplast DNA from Chlorella ellipsoidea. Plant Physiol 34: 279–310 (1982).
Yamada T, Shimaji M: Peculiar feature of the organization of rRNA genes of the Chlorella chloroplast DNA. Nucl Acids Res 14: 3827–3839 (1986).
Yelton MM, Hamer JE, Timberlake WE: Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci USA 81: 1470–1474 (1984).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schuster, A.M., Waddle, J.A., Korth, K. et al. The chloroplast genome of an exsymbiotic Chlorella-like green alga. Plant Mol Biol 14, 859–862 (1990). https://doi.org/10.1007/BF00016519
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00016519