Summary
Chloroplast DNA from species of five different conifer genera was extracted and studied by Southern blot analysis. For all these species, hybridization with heterologous probes specific for 16 S and 23 S rDNA detected only one chloroplast DNA fragment per enzyme digest. This observation suggests that the 16 S and 23 S rRNA genes are not duplicated in these genomes. The unique 16 S rDNA-containing BamHI fragment from Pinus contorta Dougl. was cloned and restriction mapped. Apart from the 16 S rRNA gene, this fragment also contained the psbC and psbD genes. It is concluded that the chloroplast genomes of a wide taxonomic range of conifers lack one of the inverted repeat elements and that a dislocation of the psbDC gene cluster has occurred in P. contorta.
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Alt J, Morris J, Westhoff P, Herrmann RG (1984) Nucleotide sequence of the clustered genes for the 44 kd chlorophyll a apoprotein and the “32 kd”-like protein of the photosystem II reaction center in the spinach plastid chromosome. Curr Genet 8:597–606
Dallimore W, Jackson AB, Harrison SG (1966) A handbook of Coniferae and Ginkgoaceae. Edward Arnold, London
Feinberg AP, Vogelstein BA (1983) A technique for radiolabelling DNA endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Hanahan D (1983) Studies on the transformation of Escherichia coli with plasmids. J Mol Biol 166:557–580
Hanahan D (1985) Techniques for transformation of E. coli. In: Glover DM (ed) DNA cloning, vol 1, a practical approach. IRL Press, Oxford, pp 109–135
Kondo T, Ishibashi T, Shibata M, Hirai A (1986) Isolation of chloroplast DNA from Pinus. Plant Cell Physiol 27:741–744
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press, New York
Neale DB, Wheeler NC, Allard RW (1986) Paternal inheritance of chloroplast DNA in Douglas-fir. Can J Forest Res 16:1152–1154
Palmer JD (1985a) Evolution of chloroplast and mitochondrial DNA in plants and algae. In: MacIntyre RJ (ed) Monographs en evolutionary biology: Molecular evolutionary genetics. Plenum, New York, pp 131–240
Palmer JD (1985b) Comparative organization of chloroplast genomes. Annu Rev Genet 19:325–354
Palmer JD, Stein DB (1987) Conservation of chloroplast genome structure among vascular plants. Curr Genet 10:823–833
Palmer JD, Thompson WF (1982) Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost. Cell 29:537–550
Rasmussen OF, Bookjans G, Stummann BM, Henningsen KW (1984) Localization and nucleotide sequence of the gene for the membrane polypeptide D2 from pea chloroplast DNA. Plant Mol Biol 3:191–199
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Szmidt AE, Lidholm J, Hällgren J-E (1986) DNA extraction and preliminary characterization of chloroplast DNA from Pinus sylvestris and Pinus contorta. In: Lindgren D (ed) Provenances and Forest Tree Breeding for High Latitudes. Proceedings of the Frans Kempe Symposium, Umeå, June 10–11, pp 269–280
Szmidt AE, Aldén T, Hällgren J-E (1987) Paternal inheritance of chloroplast DNA in Larix. Plant Mol Biol 9:59–64
Tomioka N, Sugiura M (1983) The complete nucleotide sequence of a 16 S ribosomal RNA gene from a blue-green alga, Anacystis nidulans. Mol Gen Genet 191:46–50
Wagner DB, Furnier GR, Saghai-Maroof MA, Williams SM, Dancik BP, Allard RW (1987) Chloroplast DNA polymorphisms in lodgepole and jack pine and their hybrids. Proc Natl Acad Sci USA 84:2097–2100
White EE (1986) A method for extraction of chloroplast DNA from conifers. Plant Mol Biol Rep 4:98–101
Yanisch-Perron C, Viera J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
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Communicated by R.G. Herrmann
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Lidholm, J., Szmidt, A.E., Hällgren, JE. et al. The chloroplast genomes of conifers lack one of the rRNA-encoding inverted repeats. Molec. Gen. Genet. 212, 6–10 (1988). https://doi.org/10.1007/BF00322438
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DOI: https://doi.org/10.1007/BF00322438