Abstract
Experiments were carried out to investigate the effects of root citric acid on uptake and initial distribution of cadmium (Cd) in tomato plants (Lycopersicon esculentum, cv. Tiny Tim). Cd was measured by γ-spectrometry, using 115Cd spikes. Citric acid was measured by UV-detection, and, after spiking with 14C-citric acid, by β-spectrometry. Cd was applied for 48 h periods, in control experiments, in the presence of citric acid, and after 24 h plant pre-incubation with citric acid.
Pre-incubation resulted in two-fold increases in fast-exchangeable amounts of root citric acid, as measured by the presence of citric acid in xylem exudates of decapitated and pressurized roots.
Simultaneous application of Cd and citric acid did not change Cd accumulation in total plants and in the roots, nor did any significant change occur with respect to Cd root-to-shoot transport, and Cd concentrations in shoot tissues and xylem fluid. After citric acid pre-incubation, total plant uptake of Cd increased twofold, without any significant change in Cd accumulation in the roots. Cd root-to-shoot transport was increased 5–6 fold, and Cd concentrations in shoot tissues and xylem fluid were increased 6–8 fold. Speciation calculations indicated that, under the conditions applied, xylem Cd may be, at least partly, complexed in citric acid. A C Borstlap Section editor
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Bazzaz, F A, Carlson, R W and Rolfe, G L 1974 The effect of heavy metals on plants: Part I. Inhibition of gas exchange in sunflower by Pb, Cd, Ni and Tl. Environ. Pollut. 7, 241–246.
Brown, J C 1966 Fe and Ca uptake as related to root-sap and stem-exudate citrate in soybeans. Physiol. Plant. 19, 968–976.
Brown, J C and Tiffin, L O 1964 Iron stress as related to the iron and citrate occuring in stem exudate. Plant Physiol. 40, 395–400.
Brown, J C and Chaney, R L 1971 Effect of iron on the transport of citrate into the xylem of soybeans and tomatoes. Plant Physiol. 47, 836–840.
Chaney, R L, Brown, J C and Tiffin, L O 1972 Obligatory reduction of ferric chelates in iron uptake by soybeans. Plant Physiol. 50, 208–213.
Collins, J C and Reilly, E J 1968 Chemical composition of the exudate from excised maize roots. Planta 83, 218–222.
De Bruin, M and Korthoven, P J M 1972 Computer oriented system for non-destructive neutron activation analysis. Anal. Chem. 44, 2382–2385.
De Bruin, M and Korthoven, P J M 1974 Camac based instrumentation for non-destructive neutron activation analysis system. J. Radioanal. Chem. 22, 131–138.
De Vos, C R, Lubberding, H J and Bienfait, H F 1986 Rhizosphere acidification as response to iron deficiency in bean plants. Plant Physiol. 81, 842–846.
Delhaize, E, Ryan, P R and Randall, P J 1993 Aluminium tolerance in wheat (Triticum aestivum L.). II Aluminium-stimulated excretion of malic acid from root apices. Plant Physiol. 103, 695–702.
Ernst W, Mathys W and Janiesch G 1975 Physiologische Grundlagen der Schwermetallresistenz. Forschungsber. Landes Nordrhein-Westfalen Nr 2496.
Florijn, P J and van Beusichem, M L 1993 Uptake and distribution of cadmium in maize inbred lines. Plant and Soil 150, 25–32.
Florijn, P J, Nelemans, J A and Van Beusichem, M L 1993 Evaluation of structural and physiological plant characteristics in relation to the distribution of cadmium in maize inbred lines. Plant and Soil 154, 103–109.
Godbold, D L, Horst, W J, Collins, J C, Thurman, D A and Marschner, H 1984 Accumulation of zinc and organic acids in roots of zinc tolerant and non-tolerant ecotypes of Deschampsia caespitosa. J. Plant Physiol. 16, 59–69.
Harmens H 1993 Physiology of zinc tolerance in Silene vulgaris. PhD Thesis, Vrije Universiteit Amsterdam, The Netherlands.
Hoffland, E 1992 quantitative evaluation of the role of organic acid exudation in the mobilisation of rock phosphate by rape. Plant and Soil 140, 279–289.
Kabata-Pendias, A and Pendias, H 1985 Trace Elements in Soil and Plants. CRC Press, Boca Raton, Florida.
Kishinami, I and Widholm, J M 1987 Characterization of Cu and Zn resistant Nicotiana plumbaginifolia suspension cultures. Plant Cell Physiol. 28 (3), 203–210.
Korthoven, P J M and de Bruin, M 1977 Automatic interpretation of gamma-ray data obtained in non-destructive activation analysis. J. Radioanal. Chem. 35, 127–137.
Lamoreaux, R J and Chaney, W R 1978 The effect of cadmium on net photosynthesis, transpiration and dark respiration of excised silver maple leaves. Physiol. Plant. 43, 231–236.
Landsberg, E CH 1981 Organic acid synthesis and release of hydrogen ions in response to Fe deficiency stress of mono- and dicotyledonous plant species. J. Plant Nutr. 3 (1–4), 579–591.
Lipton, D S, Blanchar, R W and Blevins, D G 1987 Citrate, malate and succinate concentration in exudates from P-sufficient and P-stressed Medicago sativa L. seedlings. Plant Physiol. 85, 315–317.
Marschner, H 1983 General introduction to the mineral nutrition of plants. In Encyclopedia of Plant Physiology, Vol 15A. Eds. A Läuchli and R L Bieleski. pp 5–60. Springer Verlag, New York.
Mathys, W 1977 The role of malate, oxalate and mustard oil glucosides in the evolution of zinc resistance in herbage plants. Physiol. Plant. 40, 130–136.
Mees, G C and Weatherley, P E 1957 The mechanism of water absorption by roots. I. Preliminary studies on the effects of hydrostatic pressure gradients Proc. R. Soc. B. 147, 367–380.
Miyasaki, S C, Buta, J G, Howell, R K and Foy, C D 1991 Mechanism of aluminium tolerance in soybeans root exudate of citric acid. Plant Physiol. 96, 737–743.
Ojima, K, Abe, H and Ohira, K 1984 Release of citric acid into medium by aluminium tolerant carrot cells. Plant Cell Physiol. 25 (5), 855–858.
Perry M W 1969 Root absorption of organic chemicals with special reference to the substituted urea herbicides. B.Sc. Honours thesis. University of Western Australia.
Perry, M W and Greenway, H 1973 Permeation of uncharged organic molecules and water through tomato roots. Ann. Bot. 37, 225–232.
Salim, M and Pitman, M G 1984 Water and solute flow through mung bean roots under applied pressure. Physiol. Plant. 61, 263–270.
Senden, M H M N and Wolterbeek, H Th 1990 Effect of citric acid on the transport of cadmium through xylem vessels of excised tomato stem-leaf systems. Acta Bot. Neerl. 39 (3), 297–303.
Senden, M H M N, Van der Meer, A J G M, Limborgh, J and Wolterbeek H Th 1992 Analysis of major tomato xylem organic acids and PITC-derivatives of amino acids by RP-HPLC and UV detection. Plant and Soil 142, 81–89.
Senden, M H M N, Van Paassen, F J M, Van der Meer, A J G M and Wolterbeek, H Th 1992a Cadmium- citric acid- xylem cell wall interactions in xylem plants. Plant Cell Environ. 15, 71–79.
Sposito, G and Coves, J 1988 Soilchem: a computer program for calculation of chemical speciation in soils. The Kearney Foundation of Soil Sciences, University of California, Berkeley, USA.
Thurman, D A and Rankin, J L 1982 The role of organic acids in zinc tolerance in Deschampsia caespitosa. New Phytol. 91, 629–635.
Tiffin, L O 1967 Translocation of manganese, iron, cobalt and zinc in tomato. Plant Physiol. 42, 1427–1432.
Tiffin, L O, Brown, J C and Krauss, R W 1960 Differential absorption of metal chelate components by plant roots. Plant Physiol. 35, 362–367.
Tonin, G S, Wheeler, C T and Crozier, A 1990 Effect of nitrogen on amino acid composition of xylem sap and stem wood in Alnus glutinosa. Physiol. Plant. 79, 506–511.
White, M C, Decker, A M and Chaney, R L 1981 Metal complexation in xylem fluid. I. Chemical composition of tomato and soybean stem exudate. Plant Physiol. 67, 292–300.
White, M C, Baker, F D, Chaney, R L and Dekker, A M 1981a Metal complexation in xylem fluid. II. Theoretical equilibrium model and computational computer program. Plant Physiol. 67, 301–310.
White, M C, Chaney, R L and Dekker, A M 1981b Metal complexation in xylem fluid. III. Electrophoretic evidence. Plant Physiol. 67, 311–315.
Wolterbeek, H Th 1987 Determination of the surface area of fine tomato roots via cation uptake experiments. Physiol. Plant. 69, 704–708.
Wolterbeek, H Th, Van der Meer, A J G M and De Bruin, M 1988 The uptake and distribution of cadmium in tomato plants affected by ethylenediamine-tetraacetic and 2,4-dinitrophenol. Environ. Pollut. 55, 301–315.
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Senden, M.H.M.N., van der Meer, A.J.G.M., Verburg, T.G. et al. Citric acid in tomato plant roots and its effect on cadmium uptake and distribution. Plant Soil 171, 333–339 (1995). https://doi.org/10.1007/BF00010289
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DOI: https://doi.org/10.1007/BF00010289