Cell suspension culture from Porphyra linearis (Rhodophyta) a multicellular marine red alga

Abstract

A cell-suspension culture suitable for continuous propagation was established from protoplasts of the red alga Porphyra linearis Grev., an edible, winter annual species of nori. Protoplast-derived cells that did not regenerate into thalli were used to establish a culture line of uniform-sized (average about 25 μm diam.) cells, which resembled the vegetative cells of this species in the leafy thallus phase. Cell division occurred about once per 24 to 30 h in uncrowded (1–2 cells per culture well) culture. This cell-suspension culture has now been maintained as continuously growing subcultures for more than four years without formation of organized thalli; however, the latter can be obtained at will by altering culture conditions (lowering temperature from 20° to 10 °C, lengthening photoperiod from 10: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaWaa0aaaeaaca% aIXaGaaGinaaaaaaa!3777!\[\overline {14} \] or 8 : % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaWaa0aaaeaaca% aIXaGaaGOnaaaaaaa!3779!\[\overline {16} \] to 14: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaWaa0aaaeaaca% aIXaGaaGimaaaaaaa!3773!\[\overline {10} \] or 16: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaWaa0aaaeaaca% aI4aaaaaaa!36C0!\[\overline 8 \] and increasing irradiance from 10 to ≥ 30 μmol m^-2 s^-1). This appears to be the first continuous non-clonal cell-suspension culture developed for a multicellular alga.