Abstract
Antioxidant compounds are increasingly attracting more attention by neuropharmacology studies due to the property of preventing brain damage. This study aimed to evaluate the supposed enhancement of antidepressant potentiality of a flavonoid-rich extract and investigate the new bioactive compounds from Passiflora edulis f. flavicarpa leaves. Quantitative analysis by RP-UHPLC-UV-DAD and extract yields were utilized in an experimental design to optimize flavonoid extraction. The total flavonoid content (11.42 mg/g), isoorientin (luteolin-6-C-β-D-glucoside) (5.21 mg/g), and LC-ESI-IT-MS/MS profile of the rich extract were determined and the flavonoid isoorientin (49 mg) was purified by preparative HPLC-UV-DAD. The antidepressant-like effects of the extract (50–100 mg/kg, p.o.) and isoorientin (1–10 mg/kg, p.o.) were investigated in the male mouse forced swimming test, in which both the positive control and experimental groups displayed reduced immobility time. Thus, the chemical results linked to the bioactivity assessments provide a 10-fold increase in the extract’s antidepressant potency and determine the relationship with the isoorientin flavonoid.
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Change history
27 March 2020
This article was updated to include the chemical structures.
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Acknowledgments
The authors express their to Mr. José Edilson de Araújo (Gurjaú Farming) for providing the plant material for the study, and to the laboratories. And finally we are grateful to colleagues of the PNBio and NPPNS research groups.
Funding
The authors received financial support from the CNPq through research grants (870079/2011-5) and fellowship (308386/2015-9), from the CAPES (AUXPE 1454/2013), and from the Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (APQ-0493-4.03/14).
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JSFA performed the chemical and behavioral experiments, analyzed the results, and wrote the manuscript; DPD performed the isolation of the chemical compounds; JGA performed the analysis by LC-MS/MS; NPL, LALS, AAdS-J, and LdSF and contributed the reagents/materials/analysis tools, helped in the identification, quantitative determination of the compounds, and designed the chemical experiments; LRFC and ECG contributed to the design and performance of in vivo behavioral experiments; SMZ, LdSF, ECG, NPL, and LALS conceived and designed the experiments, analyzed the data, interpreted the results, and wrote the manuscript. All the authors read and approved the final manuscript.
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The authors declare that the procedures followed were in accordance with the regulations of the relevant clinical research ethics committee.
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Alves, J.S.F., Marques, J.I., Demarque, D.P. et al. Involvement of Isoorientin in the Antidepressant Bioactivity of a Flavonoid-Rich Extract from Passiflora edulis f. flavicarpa Leaves. Rev. Bras. Farmacogn. 30, 240–250 (2020). https://doi.org/10.1007/s43450-020-00003-x
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DOI: https://doi.org/10.1007/s43450-020-00003-x