Journal of Analysis and Testing

, Volume 3, Issue 3, pp 253–259 | Cite as

Porous Ruthenium Selenide Nanoparticle as a Peroxidase Mimic for Glucose Bioassay

  • Wen Cao
  • Junshu Lin
  • Faheem Muhammad
  • Quan Wang
  • Xiaoyu Wang
  • Zhangping Lou
  • Hui WeiEmail author
Original Paper


Nanozyme is a promising field that offers the substitution for natural enzymes using various nanomaterials. Various nanomaterials with peroxidase-like activity were investigated. Among them, transition metal chalcogenides were explored as promising nanozymes due to their excellent enzyme-mimicking activities. However, ruthenium selenide has not been studied as a peroxidase mimic because of the difficulty for synthesis. Herein, we prepared ruthenium selenide nanomaterial with ordered mesoporous structure (P-RuSe2) employing KIT-6 silica as the template. The composition and structure of P-RuSe2 were fully characterized. Further, its peroxidase-like activity was investigated. P-RuSe2 possessed excellent peroxidase-mimicking activity, which catalyzed the oxidation of peroxidase substrates, including 3,3′,5,5′-tetramethylbenzidine, o-phenylenediamine, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in the presence of H2O2. Moreover, P-RuSe2 exhibited higher peroxidase-like activity when compared with several representative nanozymes as well as bulk RuSe2. To demonstrate its potential applications, the colorimetric detection systems for H2O2 and glucose were successfully constructed based on P-RuSe2 nanozyme.


Nanozymes Peroxidase mimics Artificial enzymes Ruthenium selenide Bionanotechnology Glucose bioassay 



This work was supported by National Natural Science Foundation of China (21874067 and 21722503), 973 Program (2015CB659400), PAPD program, Shuangchuang Program of Jiangsu Province, Open Funds of the State Key Laboratory of Analytical Chemistry for Life Science (SKLACLS1704), Open Funds of the State Key Laboratory of Coordination Chemistry (SKLCC1819), Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) (ACBM2019001), and Fundamental Research Funds for the Central Universities (021314380145).

Supplementary material

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Supplementary material 1 (DOCX 4484 kb)


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Copyright information

© The Nonferrous Metals Society of China 2019

Authors and Affiliations

  • Wen Cao
    • 1
    • 3
  • Junshu Lin
    • 1
    • 2
    • 3
  • Faheem Muhammad
    • 1
    • 3
  • Quan Wang
    • 1
    • 3
  • Xiaoyu Wang
    • 1
    • 3
  • Zhangping Lou
    • 1
    • 3
  • Hui Wei
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing National Laboratory of Microstructures, Jiangsu Key Laboratory of Artificial Functional MaterialsNanjing UniversityNanjingChina
  2. 2.Department of Biomaterials, College of MaterialsXiamen UniversityXiamenChina
  3. 3.State Key Laboratory of Analytical Chemistry for Life Science and State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical EngineeringNanjing UniversityNanjingChina

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