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Improvement of bean yield and Fusarium root rot biocontrol using mixtures of Bacillus, Pseudomonas and Rhizobium

  • Simin Kalantari
  • Alireza Marefat
  • Bita Naseri
  • Roghaie Hemmati
Original Article

Abstract

During a screening of beneficial rhizobacteria strains with capability of suppressing Fusarium root rot and improving bean yield, 680 bacteria belonging to Bacillus, fluorescent and non-fluorescent Pseudomonas, and Rhizobium were isolated from commercial field soils and plant root nodules in Zanjan province, Iran. Of these, two hundred representative isolates were biochemically characterized and screened in vitro for inhibiting mycelia growth of Fusarium solani f. sp. phaseoli. Sixteen rhizobacteria isolates were able to strongly inhibit fungal growth. Five promising rhizobacteria were evaluated for suppressing the disease and promoting plant growth in greenhouse plants. Mixed inoculation of rhizobacteria caused greater increases in disease suppression, dry and fresh matter weight of plant (aerial part) and root, plant height, and pod number per plant compared to diseased control and absolute control. Treatments involving Bacillus subtilis produced higher number of pods compared to other single and dual treatments, except for Rhizobium-Pseudomonas treatment. Treating with rhizobacteria decreased disease severity ratings by 50 to 62% compared to untreated plants. Further examination of the rhizobacteria under field conditions is required to uncover their efficacy in biofertilization and viability as biocontrol agent in bean crops.

Keywords

Legume Phaseolus vulgaris Rhizosphere Yield 

Notes

Acknowledgements

The authors acknowledged the sponsorship of the Iranian Ministry of Science, Research and Technology.

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Copyright information

© Sociedade Brasileira de Fitopatologia 2018

Authors and Affiliations

  1. 1.Department of Plant Protection, Faculty of AgricultureUniversity of ZanjanZanjanIran
  2. 2.Department of Plant Protection, Faculty of AgricultureRazi UniversityKermanshahIran
  3. 3.Plant Protection Research Department, Kermanshah Agricultural & Natural Resources Research & Education Center, AREEOKermanshahIran

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