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Association between IL-18 and IL-6 gene polymorphisms and the risk of T1D in Egyptian children

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Abstract

Purpose

To test the involvement between IL-18 and IL-6 genetic polymorphisms and susceptibility to Type 1 diabetes (T1D).

Methods

Single nucleotide polymorphisms (SNPs) at positions −607A/C and − 137G/C in IL-18 promoter region were examined by sequence specific primers-polymerase chain reaction (SSP-PCR) and position -174G/C in promoter region of IL-6 gene which analyzed by Mutagenically Separated PCR (MS-PCR) in 104 T1D participants and 114 controls.

Results

IL-18 -137GC and -137CC genotypes and -137C allele were significantly decreased in T1D subjects (P < 0.05), while -137GG genotype was insignificantly increased as compared to controls. A significant decrease was detected in haplotype -137C/-607C frequency in T1D participants compared with controls (OR = 0.04, P < 0.001). There was significant association between IL-18 -607 of (CC, AC and AA genotypes) in age at diagnosis, glycated hemoglobin (HbA1c) and higher body mass index (BMI) (P < 0.05).

Conclusion

This study demonstrated that IL-18 gene promoter polymorphisms might be associated with susceptibility to T1D in Egyptian children. Individuals carrying CC genotype at position −137 of IL-18 promoter may be at a low risk of T1D progression. Additionally, the susceptible combination of IL-18 and IL-6 cytokine genes associated with T1D highlight their risk toward the disease.

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Funding

This study was supported by Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Menoufia, Egypt.

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Authors and Affiliations

  1. Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

    Yasser B. M. Ali, Hend E. El-Gahel, Nehal E. Abdel-Hakem & Mohamed El-Shahat

  2. MolecularDiagnostics Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt

    Mahmoud E. Gadalla

  3. Biochemistry and Pediatric Departments, National Institute of Diabetes and Endocrinology (NIDE), Cairo, Egypt

    Mohamed H. El-Hefnawy

Authors
  1. Yasser B. M. Ali
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  2. Hend E. El-Gahel
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  3. Nehal E. Abdel-Hakem
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  4. Mahmoud E. Gadalla
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  5. Mohamed H. El-Hefnawy
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  6. Mohamed El-Shahat
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Contributions

Yasser, El-Gahel conceived and designed the study. El-Gahel and Abdel-Hakem participated in the experimental study and acquisition of data. El-Hefnawy and Gadalla participated in the clinical study. Yasser, El-Shahat and Abdel-Hakem analyzed and/or interpreted the data. Yasser, El-Gahel, Abdel-Hakem drafted the manuscript. Yasser and El-Shahat critically revised the manuscript for important intellectual content. All authors read and approved the final manuscript.

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Correspondence to Yasser B. M. Ali.

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Ali, Y.B.M., El-Gahel, H.E., Abdel-Hakem, N.E. et al. Association between IL-18 and IL-6 gene polymorphisms and the risk of T1D in Egyptian children. J Diabetes Metab Disord 20, 439–446 (2021). https://doi.org/10.1007/s40200-021-00763-w

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  • DOI: https://doi.org/10.1007/s40200-021-00763-w

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