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Association of polymorphisms in the IL-18 and IL-12 genes with susceptibility to Type 1 diabetes in Turkish patients

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Abstract

Background: Recent studies have indicated that polymorphisms of the interleukin-18 (IL-18) and interleukin-12 (IL-12) genes are associated with the development of Type 1 diabetes mellitus (T1 DM) in some populations, but not all. Aim: The present study was designed to examine the roles of polymorphisms in the IL-18 promoter and IL-12p40 with respect to susceptibility to T1 DM in Turkish patients. Subjects and Methods: Ninety-one patients with T1 DM and 87 unrelated healthy subjects were included in the study. The IL-18 polymorphisms at positions −607 and −137 were detected by a sequence-specific PCR method. The single nucleotide polymorphism in the IL-12p40 3′ untranslated region (3′-UTR) at position +1188 was analyzed by the PCR-restriction fragment length polymorphism (RFPL) method. Results: The allelic and genotypic frequencies of the IL-18 and IL-12p40 polymorphisms did not differ significantly between subjects with T1DM and the controls (p>0.05). However, diabetic patients with the −137 (CC) genotype showed a younger onset age compared to patients with the −137 (GG) genotype (p=0.02). In addition, patients with the −607 (CC) genotype had higher levels of glycated hemoglobin (HbA1 c) than patients with the −607 (AC) genotype (p=0.004). Furthermore, patients with the IL-12p40 (AC) genotype had higher HbA1c levels than patients with the IL-12p40 (AA) genotype (p=0.01). Conclusions: The results of the present study show that the IL-18 and IL-12p40 polymorphisms may have some effect on the onset age and deterioration of glycemic control in Turkish patients with T1 DM.

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Authors and Affiliations

  1. Department of Endocrinology and Metabolism, Gazi University Faculty of Medicine, Ankara

    A. E. Altinova, M. Akturk, F. Toruner, I. Yetkin & M. Arslan

  2. Department of Microbiology, Gazi University Faculty of Medicine, Ankara

    D. Engin

  3. Department of Endocrinology and Metabolism, Mersin University Faculty of Medicine, Mersin

    E. Akbay

  4. Department of Endocrinology and Metabolism, Ankara Ataturk Training and Research Hospital, Ankara, Turkey

    R. Ersoy

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  1. A. E. Altinova
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  2. D. Engin
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  3. E. Akbay
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  4. M. Akturk
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Correspondence to A. E. Altinova.

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Altinova, A.E., Engin, D., Akbay, E. et al. Association of polymorphisms in the IL-18 and IL-12 genes with susceptibility to Type 1 diabetes in Turkish patients. J Endocrinol Invest 33, 451–454 (2010). https://doi.org/10.1007/BF03346623

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  • DOI: https://doi.org/10.1007/BF03346623

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