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Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions

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Abstract

The aim of this work was to investigate the effect of autochthonous bioaugmentation (ABA) in phenanthrene-contaminated Patagonian soil microcosms, maintained under arid conditions, on phenanthrene elimination and soil microbial community. The polycyclic aromatic hydrocarbon (PAH)-degrading strain Sphingobium sp. 22B previously isolated from the Patagonian soil and selected by its resistance to drying conditions was used as inoculant. The phenanthrene concentration, dehydrogenase activity and denaturing gradient electrophoresis of 16S rRNA gene were monitored during 230 days. The results showed that when the microcosms were maintained at 20 % of soil water-holding capacity (WHC), the phenanthrene biodegradation was drastically inhibited and changes in the genetic diversity of soil microbial community were not detected, and neither the ABA nor the biostimulation managed to overcome the inhibitory effects. When the moisture was slightly increased, reached 25 % WHC, the ABA showed a significant initial stimulatory effect on phenanthrene biodegradation, demonstrating the potential of ABA in PAH bioremediation process in semiarid Patagonia.

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References

  • Barriuso J, Solano BR, Santamaria C, Daza A, Manero FJG (2008) Effect of inoculation with putative plant growth-promoting rhizobacteria isolated from Pinus spp. on Pinus pinea growth, mycorrhization and rhizosphere microbial communities. J Appl Microbiol 105:1298–1309

    Article  CAS  Google Scholar 

  • Chowdhury N, Marschner P, Burns RG (2011) Soil microbial activity and community composition: impact of changes in matric and osmotic potential. Soil Biol Biochem 43:1229–1236

    Article  CAS  Google Scholar 

  • Commendatore MG, Nievas ML, Amin O, Esteves JL (2012) Sources and distribution of aliphatic and polyaromatic hydrocarbons in coastal sediments from the Ushuaia Bay (Tierra del Fuego, Patagonia, Argentina). Mar Environ Res 74:20–31

    Article  CAS  Google Scholar 

  • Coppotelli BM, Ibarrolaza A, Del Panno MT, Morelli IS (2008) Effects of the inoculant strain Sphingomonas paucimobilis 20006FA on soil bacterial community biodegradation in phenanthrene-contaminated soil. Microb Ecol 55:173–183

    Article  CAS  Google Scholar 

  • Coppotelli BM, Ibarrolaza A, Dias RL, Del Panno MT, Berthe-Corti L, Morelli IS (2010) Study of the degradation activity and the strategies to promote the bioavailability of phenanthrene by Sphingomonas paucimobilis strain 20006FA. Microb Ecol 59:266–276

    Article  CAS  Google Scholar 

  • De Araujo JC, Schneider RP (2008) DGGE with genomic DNA: suitable for detection of numerically important organisms but not for identification of the most abundant organisms. Water Res 42:5002–5010

    Article  Google Scholar 

  • Del Panno MT, Morelli IS, Engelen B, Berthe-Corti L (2005) Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation. FEMS Microbiol Ecol 53:305–316

    Article  Google Scholar 

  • Fromin N, Hamelin J, Tarnawski S, Roesti D, Jourdain-Miserez K, Forestier N, Teyssier-Cuvelle S, Gillet F, Aragno M, Rossi P (2002) Statistical analysis of denaturing gel electrophoresis (DGGE) fingerprinting patterns. Environ Microbiol 4:634–643

    Article  CAS  Google Scholar 

  • Gomes N, Kosheleva I, Abraham W, Smalla K (2005) Effects of the inoculant strain Pseudomonas putida KT2442 (pNF142) and naphthalene contamination on the soil bacterial community. FEMS Microbiol Ecol 54:21–33

    Article  CAS  Google Scholar 

  • Grosser RJ, Warshawsky D, Vestal JR (1991) Indigenous and enhanced mineralization of pyrene, benzo[a]pyrene, and carbazole in soils. Appl Environ Microbiol 57:3462–3469

    CAS  Google Scholar 

  • Holman HY, Tsang YW (1995) Influence of soil moisture on biodegradation of petroleum hydrocarbons. In: Hinchee RE, Miller RN, Johnson PC (eds) In situ aeration: air sparging, bioventing, and related remediation processes. Battelle, Colombus, pp 323–332

    Google Scholar 

  • Hosokawa R, Nagai M, Morikawa M, Okuyama H (2009) Autochthonous bioaugmentation and its possible application to oil spills. World J Microbiol Biotechnol 25:1519–1528

    Article  CAS  Google Scholar 

  • Ibarrolaza A, Coppotelli BM, Del Panno MT, Donati ER, Morelli IS (2011) Application of the knowledge-based approach to strain selection for a bioaugmentation process of phenanthrene and Cr(VI)-contaminated soil. J Appl Microbiol 111:26–35

    Article  CAS  Google Scholar 

  • Ilstedt U, Nordgren A, Malmer A (2000) Optimum soil water for soil respiration before and after amendment with glucose in humid tropical acrisols and a boreal mor layer. Soil Biol Biochem 32:1591–1599

    Article  CAS  Google Scholar 

  • Ma L, Zhang J, Han L, Li W, Xu L, Hu F, Li H (2012) The effects of aging time on the fraction distribution and bioavailability of PAH. Chemosphere 86:1072–1078

    Article  CAS  Google Scholar 

  • Madueño L, Coppotelli BM, Alvarez HM, Morelli IS (2011) Isolation and characterization of indigenous soil bacteria for bioaugmentation of PAH contaminated soil of semiarid Patagonia, Argentina. Int Biodeterior Biodegrad 65:345–351

    Article  Google Scholar 

  • Muyzer G, Brinkhoff T, Nubel U, Santegoeds C, Schafer H, Wawer C (2004) Denaturing gradient gel electrophoresis (DGGE) in microbial ecology. In: Kowalchuk GA, De Bruijn FJ, Head IM, Akkermans ADL, Van Elsas JD (eds) Molecular microbial ecology manual, 2nd edn. Springer, Netherlands, pp 743–769

  • Nikolopoulou M, Pasadakis N, Kalogerakis N (2013) Evaluation of autochthonous bioaugmentation and biostimulation during microcosm-simulated oil spills. Mar Pollut Bull 72:165–173

    Article  CAS  Google Scholar 

  • Paruelo NR, Beltran A, Jobbagy E, Sala OE, Golluscio RA (1998) The climate of Patagonia: general patterns and controls on biotic processes. Ecol Austral 8:85–101

    Google Scholar 

  • Peressutti SR, Alvarez HM, Pucci OH (2003) Dynamics of hydrocarbon-degrading bacteriocenosis of an experimental oil pollution in Patagonian soil. Int Biodeterior Biodegrad 52:21–30

    Article  CAS  Google Scholar 

  • Prieto LH, Bertiller MB, Carrera AL, Olivera NL (2011) Soil enzyme and microbial activities in a grazing ecosystem of Patagonian Monte, Argentina. Geoderma 162:281–287

    Article  CAS  Google Scholar 

  • Reasoner DJ, Geldreich EE (1985) A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7

    CAS  Google Scholar 

  • Sokal RR, Michener CD (1958) A statistical method for evaluating systematic relationships. Univ Kans Sci Bull 38:1409–1438

    Google Scholar 

  • Thalman A (1968) Zur Methodik der bestimmung der ehy- drogenaseaktivita¨t im boden mittels triphenyltetra- zoliumchlorid (TTC). Landwirtsch Forsch 21:249–258

    Google Scholar 

  • Tibbett M, George SJ, Davie A, Barron A, Milton N, Greenwood PF (2011) Just add water and salt: the optimisation of petrogenic hydrocarbon biodegradation in soils from semi-arid barrow island, Western Australia. Water Air Soil Pollut 216:513–525

    Article  CAS  Google Scholar 

  • Treves DS, Xia B, Zhou J, Tiedje JM (2003) A two-species test of the hypothesis that spatial isolation influences microbial diversity in soil. Microb Ecol 45:20–28

    Article  CAS  Google Scholar 

  • Ueno A, Ito Y, Yumoto I, Okuyama H (2007) Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation. World J Microb Biotechnol 23:1739–1745

    Article  CAS  Google Scholar 

  • Viñas M, Sabate J, Espuny MJ, Anna M, Vin M (2005) Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil. Appl Environ Microbiol 71:7008–7018

    Article  Google Scholar 

  • Vogel TM (1996) Bioaugmentation as a soil bioremediation approach. Curr Opin Biotechnol 7:311–316

    Article  CAS  Google Scholar 

  • Wichern J, Wichern F, Joergensen RG (2006) Impact of salinity on soil microbial communities and the decomposition of maize in acidic soils. Geoderma 137:100–108

    Article  CAS  Google Scholar 

  • Wrenn BA, Venosa AD (1996) Selective enumeration of aromatic and aliphatic hydrocarbon degrading bacteria by a most-probable-number procedure. Can J Microbiol 42:252–258

    Article  CAS  Google Scholar 

  • Yang SZ, Jin HJ, Wei Z, He RX, Ji YJ, Li XM (2009) Bioremediation of oil spills in cold environments: a review. Pedosphere 19:371–381

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica (PICT 2010-366). Part of this work was performed in the frame of a doctoral fellowship to L. M. supported by CONICET and Oil M&S Company (R 2422). L. M. is postdoctoral fellow of CONICET. H. M. A. is research member of CONICET, and I. S. M. is research member of CIC-PBA.

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Authors and Affiliations

  1. Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI, UNLP, CCT-La Plata, CONICET, Calle 50 y 115, 1900, Buenos Aires, La Plata, Argentina

    L. Madueño & I. S. Morelli

  2. Centro Regional de Investigación y Desarrollo Científico Tecnológico (CRIDECIT), Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Comodoro Rivadavia, Chubut, Argentina

    H. M. Alvarez

  3. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA), Buenos Aires, Argentina

    I. S. Morelli

Authors
  1. L. Madueño
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  2. H. M. Alvarez
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  3. I. S. Morelli
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Correspondence to I. S. Morelli.

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Madueño, L., Alvarez, H.M. & Morelli, I.S. Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions. Int. J. Environ. Sci. Technol. 12, 2317–2326 (2015). https://doi.org/10.1007/s13762-014-0637-5

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  • DOI: https://doi.org/10.1007/s13762-014-0637-5

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