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Screening of onion accessions for Stemphylium blight resistance under artificially inoculated field experiments

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Abstract

Onion (Allium cepa L.) contributes more than 50% of the net export earnings among fresh vegetable exports in India. Stemphylium blight and purple blotch are the most destructive fungal diseases in the tropics and cause 80–90% losses in bulb and seed crop. Our aim was to screen multiple onion accessions against Stemphylium blight under artificially inoculated conditions, assess morphological and biochemical traits and correlate the observed trait with disease incidence. Screening of fifty-nine accessions resulted in identification of ‘Pusa Soumya’ (A. fistulosum L.) and ‘Red Creole2’ (A. cepa L.) as moderately resistant and ‘Red Creole1’ as susceptible. Highest marketable yield was recorded in ‘AFW’ (28.00 t/ha) followed by ‘Punjab Naroha’ (25.85 t/ha) in New Delhi, whereas in Karnal, ‘AKON555’ (45.85 t/ha) performed best. Pooled analysis revealed highest marketable yield in ‘PWF’ (44.56 t/ha). These varieties have exhibited field tolerance and in the absence of credible resistance can be recommended for areas where Stemphylium blight is the major problem. Significant variation in foliage weight, gross yield, marketable yield, dry matter, total soluble solids, total phenolic content and pyruvic acid content were recorded between non-inoculated and inoculated field trials which suggests the devastating effect of this disease on onion crop. Pearson’s correlation coefficient exhibited that percent disease index (PDI) was significantly and positively correlated with average bulb weight, gross yield, marketable yield. Significant negative correlation (r = −0.30) between dry matter and PDI (p < 0.05) and significant negative correlation (r = −0.35) between ATP (total foliar phenols after inoculation) and PDI were observed. This signifies that dry matter and total foliar phenol content can be used as a biochemical marker for high throughput screening against Stemphylium blight at preliminary screening stage. Further, ‘Pusa Soumya’ and ‘Red Creole2’ can be used as parent material to study the inheritance mechanism and for breeding of Stemphylium blight resistant onions under short day tropical conditions.

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References

  • Abang AF, Srinivasan R, Kekeunou S, Yeboah M, Hanna R, Lin MY, Tenkouano A, Bilong Bilong CF (2016) Relationship of phenotypic structures and allelochemical compounds of okra (Abelmoschus spp.) to resistance against Aphis gossypii glover. Int J Pest Manag 62:55–63

    Article  CAS  Google Scholar 

  • Abo-Elyousr KAM, Hussein MAM, Allam ADA, Hassan MH (2009) Salicylic acid induced systemic resistance on onion plants against Stemphylium vesicarium. Arch Phytopathology Plant Protect 42:1042–1050

    Article  CAS  Google Scholar 

  • Abubakar L, Ado SG (2008) Heterosis of purple blotch (Alternaria porri (Ellis) Cif.) resistance, yield and earliness in tropical onions (Allium cepa L.). Euphytica 164:63–74

    Article  Google Scholar 

  • Abubakar L, Ado SG, Suberu HA, Magaji MD (2006) Screening of onion (Allium cepa L.) cultivars for resistance to purple blotch (Alternaria porri L.) disease. Biol Environ Sci J Tropics (BEST) 3:30–36

  • Akhtari M, Dashti F, Madadi H, Rondon (2014) Evaluation of resistance to onion thrips (Thrips tabaci Lind.) in several Tareh Irani (Persian leek: Allium ampeloprasum Tareh group) landraces. Arch Phytopathology Plant Protect 47:29–41

  • Anthon GE, Barrett DM (2003) Modified method for the determination of pyruvic acid with dinitrophenylhydrazine in the assessment of onion pungency. J Sci Food Agr 83:1210–1213

    Article  CAS  Google Scholar 

  • APEDA (2017) India export of agro food products. http://agriexchange.apeda.gov.in/indexp/ Product_description_32head.aspx? gcode=0202. Accessed 26th June 2018

  • Bedford LV (1984) Dry matter and pungency tests on British grown onions. Journal of the National Institute of Agricultural Botany 16:58–61

    Google Scholar 

  • Behera S, Santra P, Chattopadhyay S, Das S, Maity TK (2013) Variation in onion varieties for reaction to natural infection of Alternaria porri (Ellis) ciff. and Stemphylium vesicarium (Wallr.). The Bioscan 8:759–761

  • Benhamou N, Gagné S, Le Quéré D, Dehbi L (2000) Bacterial-mediated induced resistance in cucumber: beneficial effect of the endophytic bacterium Serratia plymuthica on the protection against infection by Pythium ultimum. Phytopathology 90:45–56

    Article  CAS  PubMed  Google Scholar 

  • Bock KR (1964) Purple blotch (Alternaria porri) of onion in Kenya. Ann Appl Biol 54:303–311

    Article  Google Scholar 

  • Brown JKM (2006) Surveys of variation in virulence and fungicide resistance and their application to disease control. In: Cooke BM, Jones DG, Kaye B (eds) The epidemiology of plant disease. Springer, Netherland, pp 81–113

    Chapter  Google Scholar 

  • Chandrayudu E, Vemena K, Naik BS, Prathyusha C (2016) Biophysical and biochemical constituents influencing thrips and jassid resistance in groundnut germplasm, pp. 780–782. In: Peshin R, Dhawan AK, Bano F, Risam KS (eds.) Natural Resource Management: Ecological Perspectives Vol. 2. Proceedings of the Indian Ecological Society International Conference, 18–20 February 2016. Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India

  • Currah L, Maude RB (1984) Laboratory tests for leaf resistance to Botrytis squamosa in onions. Ann Appl Biol 105:277–283

    Article  Google Scholar 

  • OMB DP, Inggamer H (1984) Resistance to the onion fly in Allium cepa and Allium fistulosum.Van der Meer QP. In: Proceedings 3rd Eucarpia Allium Symp. PUDOC Wageningen, The Netherlands, pp 21–23

    Google Scholar 

  • Dhiman JS, Chadha ML, Sidhu AS (1986) Studies on the reaction of onion genotypes against purple blotch. Veg Sci 13:304–309

    Google Scholar 

  • Ellis MB (1971) Dematiaceous Hyphomycetes. Commonwealth Mycological Institute: Kew, Surrey, UK pp 608

  • FAOSTAT (2017) Onion production, area and productivity URL http://faostat3.fao.org/browse/Q/QC/E. Accessed 26 June 2018

  • Galmarini CR, Goldman IL, Havey MJ (2001) Genetic analyses of correlated solids, flavor, and health-enhancing traits in onion (Allium cepa L.). Mol Gen Genomics 265:543–551

    Article  CAS  Google Scholar 

  • Galván GA, Wietsma WA, Putrasemedja S, Permadi AH, Kik C (1997) Screening for resistance to anthracnose (Colletotrichum gloeosporioides Penz.) in Allium cepa and its wild relatives. Euphytica 95:173–178

    Article  Google Scholar 

  • Gillespie S, Long R, Seitz N, Williams N (2014) Insecticide use in hybrid onion seed production affects pre-and post pollination processes. J Econ Entomol 107:29–37

    Article  CAS  PubMed  Google Scholar 

  • Hovius MHY, Goldman IL (2002) Relationship of white rot resistance to pyruvate and S-alk (en) yl-L-cysteine sulfoxides in onion roots. In: McCreight JD, Ryder EJ (eds) XXVI international horticultural congress: advances in vegetable breeding. Acta Hortic 637:195–201

    Google Scholar 

  • Jabeen N, Ahmed N, Ghani MY, Sofi PA (2009) Role of phenolic compounds in resistance to chilli wilt. Communications in Biometry and Crop Science 4:52–61

    Google Scholar 

  • Jaime L, Martínez F, Martín-Cabrejas MA, Molla E, López-Andréu FJ, Waldron KW, Esteban RM (2001) Study of total fructan and fructooligosaccharide content in different onion tissues. J Sci Food Agric 81:177–182

    Article  CAS  Google Scholar 

  • Kamal AEAM, Mohamed HM, Aly AA, Mohamed HA (2008) Enhanced onion resistance against Stemphylium leaf blight disease, caused by Stemphylium vesicarium, by di-potassium phosphate and benzothiadiazole treatments. Plant Pathol J 24:171–177

    Article  CAS  Google Scholar 

  • Kandakoor SB, Khan HK, Chakravarthy AK, Kumar CT, Venkataravana P (2014) Biochemical constituents influencing thrips resistance in groundnut germplasm. J Environ Biol 35:675–681

    PubMed  Google Scholar 

  • Khar A, Asha Devi A, Mahajan V, Lawande KE (2006) Genetic diversity analysis in elite lines of late kharif (rangda) onion. Journal of Maharashtra Agricultural Universities 31:49–52

    Google Scholar 

  • Ko SS, Huang JW, Wang JF, Shanmugasundaram S, Chang WN (2002) Evaluation of onion cultivars for resistance to Aspergillus niger, the causal agent of black mold. J Am Soc Hortic Sci 127:697–702

    Article  Google Scholar 

  • Kumar P (2007) Genetics of resistance to Stemphylium leaf blight of lentil (Lens culinaris) in the Barimasur-4 × CSC Milestone. Dissertation, University of Saskatchewan

  • Lin MW, Watson JF, Baggett JR (1995) Inheritance of soluble solids and pyruvic acid content of bulb onions. J Am Soc Hortic Sci 120:119–122

    Article  CAS  Google Scholar 

  • Madden LV, Hughes G, van den Bosch F (2007) The study of plant disease epidemics. The American Phytopathological society. APS Press St. Paul, Minnesota

    Google Scholar 

  • Mallor Giménez C, Carravedo Fantova M, Estopañán Muñoz G, Mallor Giménez F (2011) Characterization of genetic resources of onion (Allium cepa L.) from the Spanish secondary Centre of diversity. Span J Agric Res 9:144–155

    Article  Google Scholar 

  • Mann LK, Hoyle BJ (1945) Use of the refractometer for selecting onion bulbs high in dry matter for breeding. Proc Am Soc Hortic Sci 46:285–292

    CAS  Google Scholar 

  • Maude RB (1990) Leaf disease of onion. In: Rabinowitch HD, Brewster JL (eds) Onions and Allied Crops, Vol II. Boca Raton, Florida, pp 173–190

  • McArt SH, Urbanowicz C, McCoshum S, Irwin RE, Adler LS (2017) Landscape predictors of pathogen prevalence and range contractions in US bumblebees. Proc R Soc Lond B Biol Sci 284:20172181. https://doi.org/10.1098/rspb.2017.2181

    Article  Google Scholar 

  • Miller ME, Taber RA, Amador JM (1978) Stemphylium blight of onion in South Texas. Plant Dis Rep 62:851–853

    Google Scholar 

  • Montes L (2004) Evaluation of thirty two onion cultivars in Honduras. In: Currah L (ed) International onion trial report. pp 72–73

  • Nanda S, Chand SK, Mandal P, Tripathy P, Joshi RK (2016) Identification of novel source of resistance and differential response of Allium genotypes to purple blotch pathogen, Alternaria porri (Ellis) Ciferri. Plant Pathol J 32:519–527

    Article  PubMed  PubMed Central  Google Scholar 

  • Natural Resource Institute (1990) Allium and its relatives: production and utilization, NRI Catham, UK, pp 1–189

  • Netzer D, Rabinowitch HD, Weintal CH (1985) Greenhouse technique to evaluate onion resistance to pink root. Euphytica 34:385–391

    Article  Google Scholar 

  • Nieuwhof M, De Bruyn JW, Garretsen F (1973) Methods to determine solidity and dry matter content of onions (Allium cepa L.). Euphytica 22:39–47

    Article  Google Scholar 

  • Njau GM, Nyomora AM, Dinssa FF, Chang JC, Malini P, Subramanian S, Srinivasan R (2017) Evaluation of onion (Allium cepa) germplasm entries for resistance to onion thrips, Thrips tabaci (Lindeman) in Tanzania. Int J Trop Insect Sci 37:98–113

    Article  Google Scholar 

  • Ojalvo I, Rokem JS, Navon G, Goldberg I (1987) 31P NMR study of elicitor treated Phaseolus vulgaris cell suspension cultures. Plant Physiol 85:716–719

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pathak CS, Black LL, Cheng SJ, Wang TC, Ko SS (2001) Breeding onions for Stemphylium leaf blight resistance. Acta Hortic (555):77–81. https://doi.org/10.17660/ActaHortic.2001.555.7

  • Pathak DP, Singh A, Despande A, Sridar TT (1986) Source of resistance to purple blotch in onion. Veg Sci 13:300–303

    Google Scholar 

  • Schwimmer S, Guadagni DG (1962) Relation between olfactory threshold concentration and pyruvic acid content of onion juice. J Food Sci 27:94–97

    Article  CAS  Google Scholar 

  • Shahanaz E, Razdan VK, Raina PK (2007) Survival, dispersal and management of foliar blight pathogen of onion. J Mycol Plant Pathol 37:213–214

    Google Scholar 

  • Sharma SR (1986) Effect of fungicidal on purple blotch and bulb yield of onion. Indian Phytopath 39:78–82

    CAS  Google Scholar 

  • Simon PW (1995) Genetic analysis of pungency and soluble solids in long-storage onions. Euphytica 82:1–8

    Article  CAS  Google Scholar 

  • Sinclair PJ, Blakeney AB, Barlow EWR (1995) Relationships between bulb dry matter content, soluble solids concentration and non-structural carbohydrate composition in the onion (Allium cepa). J Sci Food Agr 69:203–209

    Article  CAS  Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic 16:144–158

    CAS  Google Scholar 

  • Solanki P, Jain PK, Prajapati S, Raghuwanshi N, Khandait RN, Patel S (2015) Genetic analysis and character association in different genotypes of onion (Allium cepa L.). International Journal of Agriculture, Environment and Biotechnology 8:783–793

    Article  Google Scholar 

  • Suheri H, Price TV (2000) Infection of onion leaves by Alternaria porri and Stemphylium vesicarium and disease development in controlled environments. Plant Pathol 49:375–382

    Article  Google Scholar 

  • Tripathy P, Priyadarshini A, Das SK, Sahoo BB, Dash DK (2013) Evaluation of onion (Allium cepa L.) genotypes for tolerance to thrips (Thrips tabaci L.) and purple blotch [Alternaria Porri (Ellis) Ciferri]. International Journal of Bio-Resource and Stress Management 4:561–564

    Google Scholar 

  • Vågen IM, Slimestad R (2008) Amount of characteristic compounds in 15 cultivars of onion (Allium cepa L.) in controlled field trials. J Sci Food Agric 88:404–411

    Article  CAS  Google Scholar 

  • Wall MM, Corgan JN (1992) Relationship between pyruvate analysis and flavor perception for onion pungency determination. HortScience 27:1029–1030

    Article  CAS  Google Scholar 

  • Wheeler BEJ (1969) An introduction to plant diseases. John Wiley and Sons, London

    Google Scholar 

  • White TJ, Bruns T, Lee SJ, Taylor JL (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications. 18, pp 315–322

    Google Scholar 

  • Yoo KS, Pike L, Crosby K, Jones R, Leskovar D (2006) Differences in onion pungency due to cultivars, growth environment, and bulb sizes. Sci Hortic 110:144–149

    Article  Google Scholar 

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Authors and Affiliations

  1. Division of Vegetable Science, ICAR-Indian Agricultural Research Institute (ICAR-IARI), Pusa Campus, New Delhi, 110 012, India

    Ravindra Dangi, Sabina Islam & Anil Khar

  2. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute (ICAR-IARI), Pusa Campus, New Delhi, 110 012, India

    Parimal Sinha, Laxman Singh Rajput & Deeba Kamil

  3. ICAR-Indian Agricultural Research Institute (ICAR-IARI) Regional Station, Karnal, Haryana, 132 001, India

    Anuja Gupta

  4. Agricultural Knowledge Management Unit, ICAR-Indian Agricultural Research Institute (ICAR-IARI), Pusa Campus, New Delhi, 110 012, India

    Amrender Kumar

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  1. Ravindra Dangi
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Correspondence to Anil Khar.

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Dangi, R., Sinha, P., Islam, S. et al. Screening of onion accessions for Stemphylium blight resistance under artificially inoculated field experiments. Australasian Plant Pathol. 48, 375–384 (2019). https://doi.org/10.1007/s13313-019-00639-x

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