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In silico identification and expression analysis of superoxide dismutase (SOD) gene family in Medicago truncatula

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Abstract

Superoxide dismutase (SOD) proteins are crucial antioxidant enzymes that play critical roles in plant growth, development, and response to various abiotic stresses. The SOD gene family has been characterized in various plant species, but not in Medicago truncatula yet. Here, a total of 7 MtSOD genes were first identified from the whole genome of M. truncatula, including 1 MnSOD, 2 FeSODs, and 4 Cu/ZnSODs, which are unevenly distributed in five out of the eight chromosomes. Phylogenetic analysis showed that SOD proteins from M. truncatula and other plant species could be classified into two main categories (Cu/ZnSODs and Fe-MnSODs), which could be further divided into eight subgroups, and members within the same subgroup tended to share the same subcellular localization. In addition, MtSOD genes together with AtSODs and OsSODs within the same subgroup also displayed similar motif compositions and exon–intron structures. Most MtSOD genes were ubiquitously expressed in various tissues, particularly in leaves, seeds and root nodules at different developmental stages. Moreover, microarray analysis and high-throughput sequencing showed that most MtSOD genes were differentially expressed under salt, drought, and cold treatments, indicating their pivotal roles in stress response of M. truncatula. These findings provide useful information for the functional characterization of SOD family genes for growth, development, and stress response of M. truncatula.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31560076 and 31760074).

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Author notes

  1. Jianbo Song and Liming Zeng contributed equally to this work.

Authors and Affiliations

  1. Nanchang Economic and Technological Development District, College of Science, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China

    Jianbo Song, Liming Zeng, Rongrong Chen, Yihua Wang & Yong Zhou

  2. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China

    Yong Zhou

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  1. Jianbo Song
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  2. Liming Zeng
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  3. Rongrong Chen
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  4. Yihua Wang
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Correspondence to Yong Zhou.

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Song, J., Zeng, L., Chen, R. et al. In silico identification and expression analysis of superoxide dismutase (SOD) gene family in Medicago truncatula. 3 Biotech 8, 348 (2018). https://doi.org/10.1007/s13205-018-1373-1

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