Journal of Food Science and Technology

, Volume 51, Issue 11, pp 3464–3469

Effect of supplementation of drumstick (Moringa oleifera) and amaranth (Amaranthus tricolor) leaves powder on antioxidant profile and oxidative status among postmenopausal women

Original Article

Abstract

Menopause is a gradual three-stage process that concludes with the end of periods and reproductive life. The antioxidant enzyme system get affected in postmenopause due to deficiency of estrogen, which has got antioxidant properties. The objective of the present study was therefore, to analyze the effect of supplementation of drumstick and amaranth leaves powder on blood levels of antioxidant and marker of oxidative stress. Ninety postmenopausal women aged 45–60 years were selected and divided into three groups viz. Group I, II and III having thirty subjects in each group. The subjects of group II and III were supplemented daily with 7 g drumstick leaves powder (DLP) and 9 g amaranth leaves powder (ALP), respectively for a period of 3 months in their diet. The subjects of group I was not given supplementation. Serum retinol, serum ascorbic acid, glutathione peroxidase, superoxide dismutase and malondialdehyde were analyzed before and after supplementation. Fasting blood glucose and haemoglobin level of the subjects were also analyzed. The data revealed that supplementation of DLP and ALP significantly increased serum retinol (8.8 % and 5.0 %), serum ascorbic acid (44.4 % and 5.9 %), glutathione peroxidase (18.0 % and 11.9 %), superoxide dismutase (10.4 % and 10.8) whereas decrease in marker of oxidative stress i.e. malondialdehyde (16.3 % and 9.6 %) in postmenopausal women of group II and group III, respectively. A significant (p ≤ 0.01) decrease was also observed in fasting blood glucose level (13.5 % and 10.4 %) and increase in haemoglobin (17.5 % and 5.3 %) in group II and group III, respectively. The results indicated that these plants possess antioxidant property and have therapeutic potential for the prevention of complications during postmenopause.

Keywords

Amaranth leaves powder Antioxidant Drumstick leaves powder Oxidative stress 

References

  1. Andrews DA, Andrews K (2009) Nutraceutical Moringa composition. US Patent 0098 230Google Scholar
  2. Anilakumar KR, Khanum F, Santhanam K (2006) Amelioration of hexachlorocyclohexane-induced oxidative stress by amaranth leaves in rats. Plant Foods Hum Nutr 61:169–173CrossRefGoogle Scholar
  3. Anonymous (2011) Blood test results - normal ranges. Bloodbook.com. Cited from: http://www.bloodbook.com/ranges.html. Accessed 2 Oct 2012
  4. Arora KS, Gupta N, Singh RA, Nagpal S, Arora D (2009) Role of free radicals in menopausal distress. J Clin Diagn Res 3:1900–1902Google Scholar
  5. Bessey OA, Lowry OH, Brock J, Lopez JA (1946) The determination of vitamin A and carotene in small quantities of blood serum. J Biol Chem 166:177–188Google Scholar
  6. Dacie JV, Lewis SM (1975) Practical haematology. English Language Book Society and Churchill Ltd, London, p 321Google Scholar
  7. Esterbauer H, Chesseman KH, Dianazani MU, Poli G, Slater TF (1982) Separation and characterization of aldehydic products of LPO stimulated by ADP-Fe2+ in rat liver microsomes. Biochem J 208:129–140Google Scholar
  8. Giridhari VVA, Malathi D, Geetha K (2011) Anti diabetic property of drumstick (Moringa oleifera) leaf tablets. Int J Health Nutr 2(1):1–5Google Scholar
  9. Goyal BR, Agrawal B, Goyal RK, Mehta AA (2007) Phyto-pharmacology of Moringa oleifera Lam.o An overview. Nat Prod Radiance 6(4):347–353Google Scholar
  10. Gulati R, Bailey R, Prentice AM, Brabin BJ, Owens S (2009) Haematological effects of multimicronutrients supplementation in non-pregnant Gambian women. Eur J Clin Nutr 63(8):970–977CrossRefGoogle Scholar
  11. Hisa I, Kaji H, Inoue Y, Sugimoto T, Chihara K (2008) Fasting plasma glucose levels are related to bone mineral density in postmenopausal women with primary hyperparathyroidism. Int J Clin Exp Med 1(4):319–326Google Scholar
  12. Kaya H, Ozkaya O, Sezik M, Arslanoglu E, Yilmaztepe A, Ulukaya E (2005) Effect of raloxifene on serum malondialdehyde, erythrocyte superoxide dismutase, and erythrocyte glutathione peroxidase levels in healthy postmenopausal women. Maturitas 50:182–188CrossRefGoogle Scholar
  13. Khandaker L, Ali MB, Oba S (2008) Total polyphenol and antioxidant activity of Red Amaranth (Amaranthus tricolor L.) as affected by different sunlight level. J Japan Soc Hortic Sci 77(4):395–401CrossRefGoogle Scholar
  14. Lako J, Trenerry VC, Wahlqvist M, Wattanapenpaiboon N, Sotheeswaran S, Premier R (2007) Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods. Food Chem 101:1727–1741CrossRefGoogle Scholar
  15. Moilanen J, Aalto AM, Hemminki E, Aro AR, Raitanen J, Luoto R (2010) Prevalence of menopause symptoms and their association with lifestyle among Finnish middle-aged women. Maturitas 67:368–374CrossRefGoogle Scholar
  16. Patel S, Thakur AS, Chandy A, Manigauha A (2010) Moringa oleifera: a review of their medicinal and economical importance to the health and nation. Drug Interv Today 2(7):339–342Google Scholar
  17. Raghuram TC, Pasricha S, Sharma RD (2007) Diet and diabetes. ICMR, HyderabadGoogle Scholar
  18. Raghuramulu N, Nair KM, Kalyanasundaram S (2003) A manual of laboratory techniques. National Institute of Nutrition, HyderabadGoogle Scholar
  19. Ransel (2009) Randox Laboratories Ltd, United Kingdom, pp. 1–4Google Scholar
  20. Ransod (2009) Randox Laboratories Ltd, United Kingdom, pp.1–3Google Scholar
  21. Sachan DS, Hongu N, Johsen M (2005) Decreasing oxidative stress with Choline and Carnitine in women. J Am Coll Nutr 24(3):172–176CrossRefGoogle Scholar
  22. Srivastava V, Singh S, Neelima S, Shaila S (2005) Status of antioxidant enzymes and trace metals in postmenopausal women. J Obstet Gynecol India 55(1):64–66Google Scholar
  23. Trinder P (1969) Practical clinical biochemistry, vol X, 5th edn. William Heinnemann Medical Books Limited, New YorkGoogle Scholar

Copyright information

© Association of Food Scientists & Technologists (India) 2012

Authors and Affiliations

  • Shalini Kushwaha
    • 1
  • Paramjit Chawla
    • 1
  • Anita Kochhar
    • 1
  1. 1.Department of Food and NutritionPunjab Agricultural UniversityLudhianaIndia

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