Antioxidant capacity and phenolic content of Rumex dentatus L. Grown in Egypt
- 654 Downloads
Rumex dentatus L. (Family: Polygonaceae) is a weedy plant widely distributed in many countries including Egypt. It has been used in the Mediterranean diet as a leafy vegetable and its leaves and roots exhibited various biological activities. In our study, total phenolics, antioxidant capacities assayed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and β-carotene bleaching methods and reducing power were evaluated in different extracts/fractions of leaves and roots of R. dentatus grown in Egypt. In addition, their phenolic compositions were determined by GC-MS and HPLC. The results showed that total phenolic content in the ethyl acetate fractions of leaves and roots were high and measured at 169.5 and 257.4 mg gallic acid equivalent per g extract, respectively. The ethyl acetate fractions of leaves and roots exhibited strong DPPH activity and the DPPH IC50 values were 0.021 and 0.012 mg mL−1 of leaves and roots, respectively. Furthermore, the ethyl acetate fractions of leaves and roots showed high reducing power and antioxidant activity assayed by β-carotene bleaching method. GC-MS and HPLC analyses indicated that these fractions contained a variety of phenolic compounds including p-hydroxybenzoic acid, syringic acid, vanillin, benzoic acid, ferulic acid, and cinnamic acid. Our study verified that the ethyl acetate fractions of leaves and roots of R. dentatus have strong antioxidant activities which are correlated with its high levels of phenolic compounds and therefore, they could be utilized as a natural source of antioxidant in food industry.
Key wordsantioxidant activity GC-MS HPLC phenolic compounds Rumex dentatus L.
Unable to display preview. Download preview PDF.
- Ali M, Arfan M, Ahmad H, Zaman K, Khan F, Amarowicz R. 2011. Comparative antioxidant and antimicrobial activities of phenolic compounds extracted from five Hypericum species. Food Technol. Biotechnol. 49: 205–213Google Scholar
- Fatima N, Zia M, Riaz-ur-Rehman, Rizvil ZF, Ahmad S, Mirza B, Chaudhary MF. 2009. Biological activities of Rumex dentatus L: Evaluation of methanol and hexane extracts. Afr. J. Biotechnol. 8: 6945–6951Google Scholar
- Hameed I, Dastagir G. 2009. Nutritional analyses of Rumex hastatus D. Don, Rumex dentatus Linn and Rumex nepalensis Spreng. Afr. J. Biotechnol. 8: 4131–4133Google Scholar
- Hussain F, Ahmad B, Hameed I, Dastagir G, Sanaullah P, Azam S. 2010. Antibacterial, antifungal and insecticidal activities of some selected medicinal plants of Polygonaceae. Afr. J. Biotechnol. 9: 5032–5036Google Scholar
- Li C, Liu S. 2009. Screening of Chinese plant extracts for antioxidant activity. Mod. Pharm. Res. 2: 31–35Google Scholar
- Liu SY, Sporer F, Wink M, Jourdane J, Henning R, Li YL, Ruppel A. 1997. Anthraquinones in Rheum palmatum and Rumex dentatus (Polygonaceae), and phorbol esters in Jatropha curcas (Euphorbiaceae) with molluscicidal activity against the schistosome vector snails Oncomelania, Biomphalaria and Bulinus. Trop. Med. Int. Health. 2: 179–188PubMedCrossRefGoogle Scholar
- Loganayaki N, Siddhuraju P, Manian S. 2011. Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L. J. Food Sci. Technol. DOI 10.1007/s13197-011-0389-xGoogle Scholar
- Pourmorad F, Hosseinimehr SJ, Shahabimajd N. 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr. J. Biotechnol. 5: 1142–1145Google Scholar
- Shih FF, Diagle KW. 2003. Antioxidant properties of milledrice co-products and their effects on lipid oxidation in ground beef. Food Chem. Toxicol. 68: 2672–2675Google Scholar
- Umer A, Yousaf Z, Khan F, Hussain U, Anjum A, Nayyab Q, Younas A. 2010. Evaluation of allelopathic potential of some selected medicinal species. Afr. J. Biotechnol. 9: 6194–6206Google Scholar